Restriction map for
pF1KB7812
(Linear) (Six-base) MAPSORT of: KIBB7812.seq Check: 4243 from: 1 to: 1785
KIBB7812.orf
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
AccI GT'mk_AC
Cuts at: 0 321 1785
Size: 321 1464
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 1451 1785
Size: 64 1387 334
Fragments arranged by size:
1387 334 64
AflIII A'CryG_T
Cuts at: 0 1615 1785
Size: 1615 170
ApoI r'AATT_y
Cuts at: 0 34 78 330 469 477 581 597 665
Size: 34 44 252 139 8 104 16 68
Cuts at: 665 749 917 1337 1589 1673 1785
Size: 84 168 420 252 84 112
Fragments arranged by size:
420 252 252 168 139 112 104 84
84 68 44 34 16 8
AseI AT'TA_AT
Cuts at: 0 340 1785
Size: 340 1445
AvaI C'yCGr_G
Cuts at: 0 248 1785
Size: 248 1537
BbsI GAAGACnn'nnnn_
Cuts at: 0 114 1785
Size: 114 1671
BclI T'GATC_A
Cuts at: 0 155 1785
Size: 155 1630
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 207 779 863 947 1031 1115 1199
Size: 187 20 572 84 84 84 84 84
Cuts at: 1199 1283 1367 1535 1619 1703 1785
Size: 84 84 168 84 84 82
Fragments arranged by size:
572 187 168 84 84 84 84 84
84 84 84 84 82 20
BsaI GGTCTCn'nnnn_
Cuts at: 0 245 1785
Size: 245 1540
BsaWI w'CCGG_w
Cuts at: 0 1765 1785
Size: 1765 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 1046 1785
Size: 53 993 739
Fragments arranged by size:
993 739 53
BsrDI GCAATG_nn'
Cuts at: 0 61 1785
Size: 61 1724
BtsI GCAGTG_nn'
Cuts at: 0 65 1785
Size: 65 1720
DraI TTT'AAA
Cuts at: 0 1221 1473 1725 1785
Size: 1221 252 252 60
Fragments arranged by size:
1221 252 252 60
EaeI y'GGCC_r
Cuts at: 0 26 215 259 1785
Size: 26 189 44 1526
Fragments arranged by size:
1526 189 44 26
EarI CTCTTCn'nnn_
Cuts at: 0 190 1233 1427 1785
Size: 190 1043 194 358
Fragments arranged by size:
1043 358 194 190
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 187 207 779 863 947 1031 1115
Size: 64 123 20 572 84 84 84 84
Cuts at: 1115 1199 1283 1367 1451 1535 1619 1703 1785
Size: 84 84 84 84 84 84 84 82
Fragments arranged by size:
572 123 84 84 84 84 84 84
84 84 84 84 84 82 64 20
EcoRI G'AATT_C
Cuts at: 0 34 581 665 1673 1785
Size: 34 547 84 1008 112
Fragments arranged by size:
1008 547 112 84 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 147 179 191 223 1785
Size: 147 32 12 32 1562
Fragments arranged by size:
1562 147 32 32 12
HindIII A'AGCT_T
Cuts at: 0 878 1214 1298 1382 1550 1718 1785
Size: 878 336 84 84 168 168 67
Fragments arranged by size:
878 336 168 168 84 84 67
MscI TGG'CCA
Cuts at: 0 28 217 1785
Size: 28 189 1568
Fragments arranged by size:
1568 189 28
MslI CAynn'nnrTG
Cuts at: 0 229 1530 1785
Size: 229 1301 255
Fragments arranged by size:
1301 255 229
MspA1I CmG'CkG
Cuts at: 0 75 1785
Size: 75 1710
NspI r_CATG'y
Cuts at: 0 1619 1785
Size: 1619 166
PciI A'CATG_T
Cuts at: 0 1615 1785
Size: 1615 170
SexAI A'CCwGG_T
Cuts at: 0 116 1785
Size: 116 1669
SfcI C'TryA_G
Cuts at: 0 108 1785
Size: 108 1677
SspI AAT'ATT
Cuts at: 0 434 1785
Size: 434 1351
StuI AGG'CCT
Cuts at: 0 184 1785
Size: 184 1601
XmnI GAAnn'nnTTC
Cuts at: 0 517 1785
Size: 517 1268
Enzymes that do cut:
AccI AcuI AflIII ApoI AseI AvaI BbsI BclI
BpmI BsaI BsaWI BsgI BsrDI BtsI DraI EaeI
EarI Eco57MI EcoRI Hin4I HindIII MscI MslI MspA1I
NspI PciI SexAI SfcI SspI StuI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AsiSI
AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI
BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI
BseRI BseYI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspI FspAI HaeII
HincII HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI