Restriction map for
pF1KB7789
(Linear) (Six-base) MAPSORT of: KIBB7789.seq Check: 9452 from: 1 to: 1374
KIBB7789.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AccI GT'mk_AC
Cuts at: 0 311 1374
Size: 311 1063
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 239 1374
Size: 26 213 1135
Fragments arranged by size:
1135 213 26
AleI CACnn'nnGTG
Cuts at: 0 523 1374
Size: 523 851
AlwNI CAG_nnn'CTG
Cuts at: 0 786 1091 1374
Size: 786 305 283
Fragments arranged by size:
786 305 283
ApaI G_GGCC'C
Cuts at: 0 74 888 1374
Size: 74 814 486
Fragments arranged by size:
814 486 74
ApoI r'AATT_y
Cuts at: 0 262 1374
Size: 262 1112
AvaI C'yCGr_G
Cuts at: 0 67 73 111 233 706 1374
Size: 67 6 38 122 473 668
Fragments arranged by size:
668 473 122 67 38 6
BanI G'GyrC_C
Cuts at: 0 1335 1374
Size: 1335 39
BanII G_rGCy'C
Cuts at: 0 68 74 389 888 1016 1374
Size: 68 6 315 499 128 358
Fragments arranged by size:
499 358 315 128 68 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 466 602 1374
Size: 466 136 772
Fragments arranged by size:
772 466 136
BbvCI CC'TCA_GC
Cuts at: 0 92 1153 1374
Size: 92 1061 221
Fragments arranged by size:
1061 221 92
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 550 584 1374
Size: 550 34 790
Fragments arranged by size:
790 550 34
BglII A'GATC_T
Cuts at: 0 716 1374
Size: 716 658
BlpI GC'TnA_GC
Cuts at: 0 1075 1374
Size: 1075 299
Bme1580I G_kGCm'C
Cuts at: 0 74 888 1135 1287 1338 1374
Size: 74 814 247 152 51 36
Fragments arranged by size:
814 247 152 74 51 36
BmgBI CAC'GTC
Cuts at: 0 837 1374
Size: 837 537
BmrI ACTGGGnnnn_n'
Cuts at: 0 1281 1374
Size: 1281 93
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1045 1129 1374
Size: 1045 84 245
Fragments arranged by size:
1045 245 84
Bpu10I CC'TnA_GC
Cuts at: 0 92 379 401 1153 1374
Size: 92 287 22 752 221
Fragments arranged by size:
752 287 221 92 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 625 1312 1374
Size: 625 687 62
Fragments arranged by size:
687 625 62
BsaAI yAC'GTr
Cuts at: 0 279 1239 1374
Size: 279 960 135
Fragments arranged by size:
960 279 135
BsaBI GATnn'nnATC
Cuts at: 0 547 1374
Size: 547 827
BsaWI w'CCGG_w
Cuts at: 0 195 1374
Size: 195 1179
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 904 934 1374
Size: 904 30 440
Fragments arranged by size:
904 440 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1282 1374
Size: 1282 92
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 1374
Size: 475 899
BsiEI CG_ry'CG
Cuts at: 0 89 196 1374
Size: 89 107 1178
Fragments arranged by size:
1178 107 89
BsiHKAI G_wGCw'C
Cuts at: 0 389 1016 1033 1274 1374
Size: 389 627 17 241 100
Fragments arranged by size:
627 389 241 100 17
BsmI GAATG_Cn'
Cuts at: 0 87 210 1374
Size: 87 123 1164
Fragments arranged by size:
1164 123 87
BsmBI CGTCTCn'nnnn_
Cuts at: 0 126 1374
Size: 126 1248
Bsp1286I G_dGCh'C
Cuts at: 0 68 74 389 888 1016 1033 1135 1274
Size: 68 6 315 499 128 17 102 139
Cuts at: 1274 1287 1338 1374
Size: 13 51 36
Fragments arranged by size:
499 315 139 128 102 68 51 36
17 13 6
BsrFI r'CCGG_y
Cuts at: 0 188 290 320 875 1374
Size: 188 102 30 555 499
Fragments arranged by size:
555 499 188 102 30
BsrGI T'GTAC_A
Cuts at: 0 270 354 1374
Size: 270 84 1020
Fragments arranged by size:
1020 270 84
BssHII G'CGCG_C
Cuts at: 0 139 1374
Size: 139 1235
BstXI CCAn_nnnn'nTGG
Cuts at: 0 895 1263 1311 1374
Size: 895 368 48 63
Fragments arranged by size:
895 368 63 48
BstYI r'GATC_y
Cuts at: 0 542 716 1112 1374
Size: 542 174 396 262
Fragments arranged by size:
542 396 262 174
BtgI C'CryG_G
Cuts at: 0 975 1374
Size: 975 399
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 981 1374
Size: 981 393
DraIII CAC_nnn'GTG
Cuts at: 0 279 1374
Size: 279 1095
EaeI y'GGCC_r
Cuts at: 0 86 797 1374
Size: 86 711 577
Fragments arranged by size:
711 577 86
EagI C'GGCC_G
Cuts at: 0 86 1374
Size: 86 1288
EarI CTCTTCn'nnn_
Cuts at: 0 655 1374
Size: 655 719
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 116 1374
Size: 116 1258
EcoICRI GAG'CTC
Cuts at: 0 387 1014 1374
Size: 387 627 360
Fragments arranged by size:
627 387 360
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 239 1045 1129 1374
Size: 26 213 806 84 245
Fragments arranged by size:
806 245 213 84 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 865 1374
Size: 865 509
EcoO109I rG'GnC_Cy
Cuts at: 0 884 1149 1374
Size: 884 265 225
Fragments arranged by size:
884 265 225
EcoRI G'AATT_C
Cuts at: 0 262 1374
Size: 262 1112
HaeII r_GCGC'y
Cuts at: 0 331 1374
Size: 331 1043
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 216 248 762 794 904 936 1374
Size: 216 32 514 32 110 32 438
Fragments arranged by size:
514 438 216 110 32 32 32
HindIII A'AGCT_T
Cuts at: 0 802 1374
Size: 802 572
MscI TGG'CCA
Cuts at: 0 799 1374
Size: 799 575
MslI CAynn'nnrTG
Cuts at: 0 523 739 1309 1374
Size: 523 216 570 65
Fragments arranged by size:
570 523 216 65
MspA1I CmG'CkG
Cuts at: 0 91 977 1056 1374
Size: 91 886 79 318
Fragments arranged by size:
886 318 91 79
NaeI GCC'GGC
Cuts at: 0 190 1374
Size: 190 1184
NgoMIV G'CCGG_C
Cuts at: 0 188 1374
Size: 188 1186
NotI GC'GGCC_GC
Cuts at: 0 86 1374
Size: 86 1288
PflMI CCAn_nnn'nTGG
Cuts at: 0 620 1374
Size: 620 754
PfoI T'CCnGG_A
Cuts at: 0 809 1374
Size: 809 565
PmlI CAC'GTG
Cuts at: 0 1239 1374
Size: 1239 135
PpuMI rG'GwC_Cy
Cuts at: 0 1149 1374
Size: 1149 225
PspOMI G'GGCC_C
Cuts at: 0 70 884 1374
Size: 70 814 490
Fragments arranged by size:
814 490 70
SacI G_AGCT'C
Cuts at: 0 389 1016 1374
Size: 389 627 358
Fragments arranged by size:
627 389 358
SacII CC_GC'GG
Cuts at: 0 978 1374
Size: 978 396
SapI GCTCTTCn'nnn_
Cuts at: 0 655 1374
Size: 655 719
ScaI AGT'ACT
Cuts at: 0 634 1222 1374
Size: 634 588 152
Fragments arranged by size:
634 588 152
SmaI CCC'GGG
Cuts at: 0 69 75 113 708 1374
Size: 69 6 38 595 666
Fragments arranged by size:
666 595 69 38 6
SmlI C'TyrA_G
Cuts at: 0 233 640 1327 1374
Size: 233 407 687 47
Fragments arranged by size:
687 407 233 47
SrfI GCCC'GGGC
Cuts at: 0 69 1374
Size: 69 1305
StyI C'CwwG_G
Cuts at: 0 494 521 908 1374
Size: 494 27 387 466
Fragments arranged by size:
494 466 387 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1089 1189 1269 1317 1374
Size: 1089 100 80 48 57
Fragments arranged by size:
1089 100 80 57 48
TatI w'GTAC_w
Cuts at: 0 270 354 632 644 827 1220 1374
Size: 270 84 278 12 183 393 154
Fragments arranged by size:
393 278 270 183 154 84 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 923 955 1374
Size: 923 32 419
Fragments arranged by size:
923 419 32
Tth111I GACn'n_nGTC
Cuts at: 0 1318 1374
Size: 1318 56
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 492 807 1374
Size: 492 315 567
Fragments arranged by size:
567 492 315
XhoI C'TCGA_G
Cuts at: 0 233 1374
Size: 233 1141
XmaI C'CCGG_G
Cuts at: 0 67 73 111 706 1374
Size: 67 6 38 595 668
Fragments arranged by size:
668 595 67 38 6
Enzymes that do cut:
AccI AcuI AleI AlwNI ApaI ApoI AvaI BanI
BanII BbsI BbvCI BcgI BglII BlpI Bme1580I BmgBI
BmrI BpmI Bpu10I BpuEI BsaAI BsaBI BsaWI BsaXI
BseRI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BsrFI
BsrGI BssHII BstXI BstYI BtgI BtgZI DraIII EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
HaeII Hin4I HindIII MscI MslI MspA1I NaeI NgoMIV
NotI PflMI PfoI PmlI PpuMI PspOMI SacI SacII
SapI ScaI SmaI SmlI SrfI StyI TaqII TatI
TstI Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BciVI BclI BfrBI BglI BmtI
BplI BsaI BsaHI BseYI BsiWI BspEI BspHI BspMI
BsrBI BsrDI BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
BtsI ClaI CspCI DraI DrdI EcoRV FalI FseI
FspI FspAI HincII HpaI KasI KpnI MfeI MluI
MmeI NarI NcoI NdeI NheI NruI NsiI NspI
PacI PasI PciI PmeI PpiI PshAI PsiI PspXI
PsrI PstI PvuI PvuII RsrII SalI SanDI SbfI
SexAI SfcI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SspI StuI SwaI XbaI XmnI ZraI