Restriction map for
pF1KB7788
(Linear) (Six-base) MAPSORT of: KIBB7788.seq Check: 2772 from: 1 to: 1542
KIBB7788.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AccI GT'mk_AC
Cuts at: 0 1002 1542
Size: 1002 540
AclI AA'CG_TT
Cuts at: 0 1485 1542
Size: 1485 57
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 740 853 1493 1542
Size: 169 571 113 640 49
Fragments arranged by size:
640 571 169 113 49
AleI CACnn'nnGTG
Cuts at: 0 660 1542
Size: 660 882
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 476 508 1542
Size: 476 32 1034
Fragments arranged by size:
1034 476 32
AlwNI CAG_nnn'CTG
Cuts at: 0 559 1273 1542
Size: 559 714 269
Fragments arranged by size:
714 559 269
ApoI r'AATT_y
Cuts at: 0 117 1542
Size: 117 1425
AseI AT'TA_AT
Cuts at: 0 1028 1542
Size: 1028 514
BbsI GAAGACnn'nnnn_
Cuts at: 0 22 1542
Size: 22 1520
BciVI GTATCCnnnnn_n'
Cuts at: 0 1376 1542
Size: 1376 166
BglII A'GATC_T
Cuts at: 0 741 1177 1542
Size: 741 436 365
Fragments arranged by size:
741 436 365
Bpu10I CC'TnA_GC
Cuts at: 0 1266 1542
Size: 1266 276
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 32 62 1542
Size: 32 30 1480
Fragments arranged by size:
1480 32 30
BsiHKAI G_wGCw'C
Cuts at: 0 385 1542
Size: 385 1157
BsmI GAATG_Cn'
Cuts at: 0 755 965 1320 1542
Size: 755 210 355 222
Fragments arranged by size:
755 355 222 210
Bsp1286I G_dGCh'C
Cuts at: 0 385 1542
Size: 385 1157
BsrGI T'GTAC_A
Cuts at: 0 408 1445 1542
Size: 408 1037 97
Fragments arranged by size:
1037 408 97
BstAPI GCAn_nnn'nTGC
Cuts at: 0 569 1542
Size: 569 973
BstBI TT'CG_AA
Cuts at: 0 475 1542
Size: 475 1067
BstYI r'GATC_y
Cuts at: 0 741 1177 1542
Size: 741 436 365
Fragments arranged by size:
741 436 365
Bsu36I CC'TnA_GG
Cuts at: 0 75 1542
Size: 75 1467
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1007 1542
Size: 1007 535
BtsI GCAGTG_nn'
Cuts at: 0 112 385 1542
Size: 112 273 1157
Fragments arranged by size:
1157 273 112
DraI TTT'AAA
Cuts at: 0 1062 1490 1542
Size: 1062 428 52
Fragments arranged by size:
1062 428 52
DrdI GACnn_nn'nnGTC
Cuts at: 0 1160 1542
Size: 1160 382
EarI CTCTTCn'nnn_
Cuts at: 0 472 474 1230 1427 1501 1542
Size: 472 2 756 197 74 41
Fragments arranged by size:
756 472 197 74 41 2
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 740 853 1493 1542
Size: 169 571 113 640 49
Fragments arranged by size:
640 571 169 113 49
EcoO109I rG'GnC_Cy
Cuts at: 0 1153 1542
Size: 1153 389
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 284 1542
Size: 284 1258
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 541 573 831 863 1542
Size: 541 32 258 32 679
Fragments arranged by size:
679 541 258 32 32
HincII GTy'rAC
Cuts at: 0 839 1542
Size: 839 703
HindIII A'AGCT_T
Cuts at: 0 942 1542
Size: 942 600
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1483 1542
Size: 1483 59
MslI CAynn'nnrTG
Cuts at: 0 443 660 1019 1081 1393 1542
Size: 443 217 359 62 312 149
Fragments arranged by size:
443 359 312 217 149 62
MspA1I CmG'CkG
Cuts at: 0 228 1542
Size: 228 1314
NruI TCG'CGA
Cuts at: 0 352 1542
Size: 352 1190
NspI r_CATG'y
Cuts at: 0 222 576 1542
Size: 222 354 966
Fragments arranged by size:
966 354 222
PfoI T'CCnGG_A
Cuts at: 0 1008 1542
Size: 1008 534
PpuMI rG'GwC_Cy
Cuts at: 0 1153 1542
Size: 1153 389
PstI C_TGCA'G
Cuts at: 0 107 1098 1542
Size: 107 991 444
Fragments arranged by size:
991 444 107
PvuII CAG'CTG
Cuts at: 0 228 1542
Size: 228 1314
SexAI A'CCwGG_T
Cuts at: 0 1155 1542
Size: 1155 387
SfcI C'TryA_G
Cuts at: 0 103 1094 1542
Size: 103 991 448
Fragments arranged by size:
991 448 103
StyI C'CwwG_G
Cuts at: 0 1401 1542
Size: 1401 141
TatI w'GTAC_w
Cuts at: 0 39 408 828 1445 1542
Size: 39 369 420 617 97
Fragments arranged by size:
617 420 369 97 39
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1263 1295 1542
Size: 1263 32 247
Fragments arranged by size:
1263 247 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 266 1542
Size: 266 1276
XmnI GAAnn'nnTTC
Cuts at: 0 743 1318 1521 1542
Size: 743 575 203 21
Fragments arranged by size:
743 575 203 21
Enzymes that do cut:
AccI AclI AcuI AleI AloI AlwNI ApoI AseI
BbsI BciVI BglII Bpu10I BsaXI BsiHKAI BsmI Bsp1286I
BsrGI BstAPI BstBI BstYI Bsu36I BtgZI BtsI DraI
DrdI EarI Eco57MI EcoO109I FalI Hin4I HincII HindIII
MmeI MslI MspA1I NruI NspI PfoI PpuMI PstI
PvuII SexAI SfcI StyI TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AflIII AgeI AhdI
AlfI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI
BamHI BanI BanII BbeI BbvCI BcgI BclI BfrBI
BglI BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstEII BstXI BstZ17I BtgI
ClaI CspCI DraIII EaeI EagI EciI EcoICRI EcoNI
EcoRI EcoRV FseI FspI FspAI HaeII HpaI KasI
KpnI MfeI MluI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI PacI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII Tth111I
XbaI XhoI XmaI ZraI