Restriction map for
pF1KB7785
(Linear) (Six-base) MAPSORT of: KIBB7785.seq Check: 3854 from: 1 to: 1371
KIBB7785.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AccI GT'mk_AC
Cuts at: 0 513 1371
Size: 513 858
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 1164 1248 1371
Size: 52 1112 84 123
Fragments arranged by size:
1112 123 84 52
ApoI r'AATT_y
Cuts at: 0 317 794 1046 1332 1371
Size: 317 477 252 286 39
Fragments arranged by size:
477 317 286 252 39
BanII G_rGCy'C
Cuts at: 0 1276 1371
Size: 1276 95
BclI T'GATC_A
Cuts at: 0 305 1371
Size: 305 1066
BfrBI ATG'CAT
Cuts at: 0 568 908 1108 1371
Size: 568 340 200 263
Fragments arranged by size:
568 340 263 200
BglII A'GATC_T
Cuts at: 0 219 1371
Size: 219 1152
BmrI ACTGGGnnnn_n'
Cuts at: 0 142 291 728 1371
Size: 142 149 437 643
Fragments arranged by size:
643 437 149 142
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1081 1371
Size: 1081 290
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 572 908 1076 1160 1244 1290 1371
Size: 572 336 168 84 84 46 81
Fragments arranged by size:
572 336 168 84 84 81 46
BsaI GGTCTCn'nnnn_
Cuts at: 0 132 1371
Size: 132 1239
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 67 1371
Size: 67 1304
BseYI C'CCAG_C
Cuts at: 0 195 1371
Size: 195 1176
BsiHKAI G_wGCw'C
Cuts at: 0 1276 1371
Size: 1276 95
BsmI GAATG_Cn'
Cuts at: 0 910 1371
Size: 910 461
Bsp1286I G_dGCh'C
Cuts at: 0 1276 1371
Size: 1276 95
BsrGI T'GTAC_A
Cuts at: 0 89 1371
Size: 89 1282
BstXI CCAn_nnnn'nTGG
Cuts at: 0 496 1371
Size: 496 875
BstYI r'GATC_y
Cuts at: 0 219 1371
Size: 219 1152
BtsI GCAGTG_nn'
Cuts at: 0 855 1371
Size: 855 516
DraI TTT'AAA
Cuts at: 0 351 1371
Size: 351 1020
EaeI y'GGCC_r
Cuts at: 0 32 117 1371
Size: 32 85 1254
Fragments arranged by size:
1254 85 32
EarI CTCTTCn'nnn_
Cuts at: 0 1123 1371
Size: 1123 248
EcoICRI GAG'CTC
Cuts at: 0 1274 1371
Size: 1274 97
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 572 908 1076 1160 1164 1244 1248
Size: 52 520 336 168 84 4 80 4
Cuts at: 1248 1290 1371
Size: 42 81
Fragments arranged by size:
520 336 168 84 81 80 52 42
4 4
EcoO109I rG'GnC_Cy
Cuts at: 0 331 476 1371
Size: 331 145 895
Fragments arranged by size:
895 331 145
EcoRI G'AATT_C
Cuts at: 0 317 794 1046 1371
Size: 317 477 252 325
Fragments arranged by size:
477 325 317 252
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 277 309 1290 1322 1371
Size: 277 32 981 32 49
Fragments arranged by size:
981 277 49 32 32
HincII GTy'rAC
Cuts at: 0 514 1371
Size: 514 857
HindIII A'AGCT_T
Cuts at: 0 1007 1371
Size: 1007 364
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 86 797 1371
Size: 86 711 574
Fragments arranged by size:
711 574 86
MscI TGG'CCA
Cuts at: 0 34 119 1371
Size: 34 85 1252
Fragments arranged by size:
1252 85 34
MslI CAynn'nnrTG
Cuts at: 0 494 567 1371
Size: 494 73 804
Fragments arranged by size:
804 494 73
MspA1I CmG'CkG
Cuts at: 0 1268 1371
Size: 1268 103
NdeI CA'TA_TG
Cuts at: 0 452 564 1371
Size: 452 112 807
Fragments arranged by size:
807 452 112
NsiI A_TGCA'T
Cuts at: 0 570 910 1110 1371
Size: 570 340 200 261
Fragments arranged by size:
570 340 261 200
NspI r_CATG'y
Cuts at: 0 572 1108 1371
Size: 572 536 263
Fragments arranged by size:
572 536 263
PvuII CAG'CTG
Cuts at: 0 1268 1371
Size: 1268 103
SacI G_AGCT'C
Cuts at: 0 1276 1371
Size: 1276 95
SalI G'TCGA_C
Cuts at: 0 512 1371
Size: 512 859
ScaI AGT'ACT
Cuts at: 0 465 1371
Size: 465 906
SphI G_CATG'C
Cuts at: 0 1108 1371
Size: 1108 263
StuI AGG'CCT
Cuts at: 0 757 1371
Size: 757 614
TatI w'GTAC_w
Cuts at: 0 89 463 739 1371
Size: 89 374 276 632
Fragments arranged by size:
632 374 276 89
XmnI GAAnn'nnTTC
Cuts at: 0 317 863 1009 1371
Size: 317 546 146 362
Fragments arranged by size:
546 362 317 146
Enzymes that do cut:
AccI AcuI ApoI BanII BclI BfrBI BglII BmrI
BplI BpmI BsaI BseRI BseYI BsiHKAI BsmI Bsp1286I
BsrGI BstXI BstYI BtsI DraI EaeI EarI EcoICRI
Eco57MI EcoO109I EcoRI Hin4I HincII HindIII MmeI MscI
MslI MspA1I NdeI NsiI NspI PvuII SacI SalI
ScaI SphI StuI TatI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI
BbsI BbvCI BcgI BciVI BglI BlpI Bme1580I BmgBI
BmtI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI
BsgI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstZ17I
Bsu36I BtgI BtgZI ClaI CspCI DraIII DrdI EagI
EciI EcoNI EcoRV FalI FseI FspI FspAI HaeII
HpaI KasI KpnI MfeI MluI NaeI NarI NcoI
NgoMIV NheI NotI NruI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacII SanDI SapI
SbfI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SrfI SspI StyI SwaI TaqII
TstI Tth111I XbaI XcmI XhoI XmaI ZraI