Restriction map for
pF1KB7775
(Linear) (Six-base) MAPSORT of: KIBB7775.seq Check: 158 from: 1 to: 1359
KIBB7775.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
Acc65I G'GTAC_C
Cuts at: 0 557 1359
Size: 557 802
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 1207 1359
Size: 215 992 152
Fragments arranged by size:
992 215 152
ApoI r'AATT_y
Cuts at: 0 151 461 716 1359
Size: 151 310 255 643
Fragments arranged by size:
643 310 255 151
BamHI G'GATC_C
Cuts at: 0 1203 1359
Size: 1203 156
BanI G'GyrC_C
Cuts at: 0 26 557 1359
Size: 26 531 802
Fragments arranged by size:
802 531 26
BbeI G_GCGC'C
Cuts at: 0 30 1359
Size: 30 1329
BbsI GAAGACnn'nnnn_
Cuts at: 0 918 1359
Size: 918 441
BciVI GTATCCnnnnn_n'
Cuts at: 0 1069 1359
Size: 1069 290
BclI T'GATC_A
Cuts at: 0 350 1359
Size: 350 1009
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 1359
Size: 410 949
Bpu10I CC'TnA_GC
Cuts at: 0 763 1359
Size: 763 596
BsaBI GATnn'nnATC
Cuts at: 0 860 1359
Size: 860 499
BsaHI Gr'CG_yC
Cuts at: 0 27 37 1359
Size: 27 10 1322
Fragments arranged by size:
1322 27 10
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1241 1271 1359
Size: 1241 30 88
Fragments arranged by size:
1241 88 30
BseYI C'CCAG_C
Cuts at: 0 547 1359
Size: 547 812
BspHI T'CATG_A
Cuts at: 0 491 1359
Size: 491 868
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1035 1359
Size: 1035 324
BsrGI T'GTAC_A
Cuts at: 0 658 1359
Size: 658 701
BstYI r'GATC_y
Cuts at: 0 690 1146 1203 1359
Size: 690 456 57 156
Fragments arranged by size:
690 456 156 57
BtgI C'CryG_G
Cuts at: 0 1137 1359
Size: 1137 222
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 361 1359
Size: 361 998
ClaI AT'CG_AT
Cuts at: 0 1274 1359
Size: 1274 85
EaeI y'GGCC_r
Cuts at: 0 837 1359
Size: 837 522
EarI CTCTTCn'nnn_
Cuts at: 0 1226 1359
Size: 1226 133
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 410 1207 1359
Size: 215 195 797 152
Fragments arranged by size:
797 215 195 152
EcoRI G'AATT_C
Cuts at: 0 151 1359
Size: 151 1208
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 34 523 696 1005 1359
Size: 34 489 173 309 354
Fragments arranged by size:
489 354 309 173 34
HaeII r_GCGC'y
Cuts at: 0 30 1359
Size: 30 1329
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 763 795 1359
Size: 763 32 564
Fragments arranged by size:
763 564 32
KasI G'GCGC_C
Cuts at: 0 26 1359
Size: 26 1333
KpnI G_GTAC'C
Cuts at: 0 561 1359
Size: 561 798
MscI TGG'CCA
Cuts at: 0 839 1359
Size: 839 520
NarI GG'CG_CC
Cuts at: 0 27 1359
Size: 27 1332
NcoI C'CATG_G
Cuts at: 0 1137 1359
Size: 1137 222
NdeI CA'TA_TG
Cuts at: 0 453 1359
Size: 453 906
NspI r_CATG'y
Cuts at: 0 737 876 1359
Size: 737 139 483
Fragments arranged by size:
737 483 139
PfoI T'CCnGG_A
Cuts at: 0 944 1359
Size: 944 415
PshAI GACnn'nnGTC
Cuts at: 0 922 1359
Size: 922 437
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1060 1092 1359
Size: 1060 32 267
Fragments arranged by size:
1060 267 32
PstI C_TGCA'G
Cuts at: 0 1032 1344 1359
Size: 1032 312 15
Fragments arranged by size:
1032 312 15
ScaI AGT'ACT
Cuts at: 0 844 1359
Size: 844 515
SfcI C'TryA_G
Cuts at: 0 211 1028 1340 1359
Size: 211 817 312 19
Fragments arranged by size:
817 312 211 19
SfoI GGC'GCC
Cuts at: 0 28 1359
Size: 28 1331
SphI G_CATG'C
Cuts at: 0 737 876 1359
Size: 737 139 483
Fragments arranged by size:
737 483 139
SspI AAT'ATT
Cuts at: 0 112 1359
Size: 112 1247
StyI C'CwwG_G
Cuts at: 0 782 1137 1359
Size: 782 355 222
Fragments arranged by size:
782 355 222
TatI w'GTAC_w
Cuts at: 0 658 842 1359
Size: 658 184 517
Fragments arranged by size:
658 517 184
Tth111I GACn'n_nGTC
Cuts at: 0 997 1359
Size: 997 362
XmnI GAAnn'nnTTC
Cuts at: 0 17 462 676 1285 1359
Size: 17 445 214 609 74
Fragments arranged by size:
609 445 214 74 17
Enzymes that do cut:
Acc65I AcuI ApoI BamHI BanI BbeI BbsI BciVI
BclI BpmI Bpu10I BsaBI BsaHI BsaXI BseYI BspHI
BspMI BsrGI BstYI BtgI BtgZI ClaI EaeI EarI
Eco57MI EcoRI FalI HaeII Hin4I KasI KpnI MscI
NarI NcoI NdeI NspI PfoI PshAI PsrI PstI
ScaI SfcI SfoI SphI SspI StyI TatI Tth111I
XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BanII BbvCI BcgI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpuEI BsaI BsaAI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtsI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoO109I EcoRV FseI FspI FspAI HincII
HindIII HpaI MfeI MluI MmeI MslI MspA1I NaeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SrfI StuI SwaI TaqII TstI XbaI XcmI
XhoI XmaI ZraI