Restriction map for
pF1KB7771
(Linear) (Six-base) MAPSORT of: KIBB7771.seq Check: 4230 from: 1 to: 1350
KIBB7771.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 803 941 1350
Size: 803 138 409
Fragments arranged by size:
803 409 138
AflIII A'CryG_T
Cuts at: 0 186 1350
Size: 186 1164
AhdI GACnn_n'nnGTC
Cuts at: 0 1071 1350
Size: 1071 279
ApoI r'AATT_y
Cuts at: 0 283 631 1350
Size: 283 348 719
Fragments arranged by size:
719 348 283
BanII G_rGCy'C
Cuts at: 0 306 1350
Size: 306 1044
Bme1580I G_kGCm'C
Cuts at: 0 1221 1350
Size: 1221 129
BmrI ACTGGGnnnn_n'
Cuts at: 0 565 1350
Size: 565 785
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 712 1350
Size: 712 638
Bpu10I CC'TnA_GC
Cuts at: 0 480 1350
Size: 480 870
BsaI GGTCTCn'nnnn_
Cuts at: 0 265 1350
Size: 265 1085
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1164 1194 1350
Size: 1164 30 156
Fragments arranged by size:
1164 156 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 780 783 786 789 1304 1350
Size: 780 3 3 3 515 46
Fragments arranged by size:
780 515 46 3 3 3
BsiHKAI G_wGCw'C
Cuts at: 0 1313 1350
Size: 1313 37
Bsp1286I G_dGCh'C
Cuts at: 0 306 1221 1313 1350
Size: 306 915 92 37
Fragments arranged by size:
915 306 92 37
BspHI T'CATG_A
Cuts at: 0 954 1350
Size: 954 396
BsrGI T'GTAC_A
Cuts at: 0 1301 1350
Size: 1301 49
BssSI C'ACGA_G
Cuts at: 0 308 1021 1350
Size: 308 713 329
Fragments arranged by size:
713 329 308
BstBI TT'CG_AA
Cuts at: 0 369 1350
Size: 369 981
BstYI r'GATC_y
Cuts at: 0 618 1350
Size: 618 732
BtgI C'CryG_G
Cuts at: 0 1140 1269 1350
Size: 1140 129 81
Fragments arranged by size:
1140 129 81
ClaI AT'CG_AT
Cuts at: 0 490 1350
Size: 490 860
DraI TTT'AAA
Cuts at: 0 638 1350
Size: 638 712
EarI CTCTTCn'nnn_
Cuts at: 0 733 777 807 917 937 1096 1232 1350
Size: 733 44 30 110 20 159 136 118
Fragments arranged by size:
733 159 136 118 110 44 30 20
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 712 803 941 1350
Size: 712 91 138 409
Fragments arranged by size:
712 409 138 91
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 54 1350
Size: 54 1296
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1057 1089 1164 1196 1350
Size: 1057 32 75 32 154
Fragments arranged by size:
1057 154 75 32 32
HincII GTy'rAC
Cuts at: 0 411 1350
Size: 411 939
HindIII A'AGCT_T
Cuts at: 0 949 1350
Size: 949 401
NcoI C'CATG_G
Cuts at: 0 1269 1350
Size: 1269 81
NdeI CA'TA_TG
Cuts at: 0 99 156 1350
Size: 99 57 1194
Fragments arranged by size:
1194 99 57
SapI GCTCTTCn'nnn_
Cuts at: 0 733 1232 1350
Size: 733 499 118
Fragments arranged by size:
733 499 118
StyI C'CwwG_G
Cuts at: 0 995 1269 1350
Size: 995 274 81
Fragments arranged by size:
995 274 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 289 1328 1350
Size: 289 1039 22
Fragments arranged by size:
1039 289 22
TatI w'GTAC_w
Cuts at: 0 552 1301 1350
Size: 552 749 49
Fragments arranged by size:
749 552 49
XmnI GAAnn'nnTTC
Cuts at: 0 10 1350
Size: 10 1340
Enzymes that do cut:
AcuI AflIII AhdI ApoI BanII Bme1580I BmrI BpmI
Bpu10I BsaI BsaXI BseRI BsiHKAI Bsp1286I BspHI BsrGI
BssSI BstBI BstYI BtgI ClaI DraI EarI Eco57MI
FalI Hin4I HincII HindIII NcoI NdeI SapI StyI
TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
BmgBI BmtI BplI BpuEI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspMI
BsrBI BsrDI BsrFI BssHII BstAPI BstEII BstXI BstZ17I
Bsu36I BtgZI BtsI CspCI DraIII DrdI EaeI EagI
EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI FspI
FspAI HaeII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TstI Tth111I XbaI XcmI XhoI XmaI
ZraI