Restriction map for
pF1KB7770
(Linear) (Six-base) MAPSORT of: KIBB7770.seq Check: 7878 from: 1 to: 1431
KIBB7770.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1350 1431
Size: 1350 81
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1282 1431
Size: 1282 149
AflIII A'CryG_T
Cuts at: 0 1344 1431
Size: 1344 87
AhdI GACnn_n'nnGTC
Cuts at: 0 1333 1431
Size: 1333 98
AlwNI CAG_nnn'CTG
Cuts at: 0 1356 1431
Size: 1356 75
ApaI G_GGCC'C
Cuts at: 0 639 1431
Size: 639 792
ApoI r'AATT_y
Cuts at: 0 756 1431
Size: 756 675
BamHI G'GATC_C
Cuts at: 0 458 1431
Size: 458 973
BanII G_rGCy'C
Cuts at: 0 639 1097 1256 1431
Size: 639 458 159 175
Fragments arranged by size:
639 458 175 159
BbsI GAAGACnn'nnnn_
Cuts at: 0 83 158 277 1226 1378 1384 1431
Size: 83 75 119 949 152 6 47
Fragments arranged by size:
949 152 119 83 75 47 6
BclI T'GATC_A
Cuts at: 0 1364 1431
Size: 1364 67
BglI GCCn_nnn'nGGC
Cuts at: 0 30 1431
Size: 30 1401
BglII A'GATC_T
Cuts at: 0 587 1412 1431
Size: 587 825 19
Fragments arranged by size:
825 587 19
Bme1580I G_kGCm'C
Cuts at: 0 639 1431
Size: 639 792
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 111 576 1240 1270 1291 1431
Size: 111 465 664 30 21 140
Fragments arranged by size:
664 465 140 111 30 21
BsaI GGTCTCn'nnnn_
Cuts at: 0 1178 1431
Size: 1178 253
BsaAI yAC'GTr
Cuts at: 0 1347 1431
Size: 1347 84
BsaWI w'CCGG_w
Cuts at: 0 404 1431
Size: 404 1027
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 73 103 562 592 1084 1114 1431
Size: 73 30 459 30 492 30 317
Fragments arranged by size:
492 459 317 73 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 940 1431
Size: 940 491
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 898 1369 1431
Size: 898 471 62
Fragments arranged by size:
898 471 62
BsiHKAI G_wGCw'C
Cuts at: 0 1256 1431
Size: 1256 175
BsmI GAATG_Cn'
Cuts at: 0 905 1431
Size: 905 526
Bsp1286I G_dGCh'C
Cuts at: 0 639 1097 1256 1431
Size: 639 458 159 175
Fragments arranged by size:
639 458 175 159
BspEI T'CCGG_A
Cuts at: 0 404 1431
Size: 404 1027
BspHI T'CATG_A
Cuts at: 0 294 1040 1431
Size: 294 746 391
Fragments arranged by size:
746 391 294
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1244 1350 1431
Size: 1244 106 81
Fragments arranged by size:
1244 106 81
BsrBI CCG'CTC
Cuts at: 0 1401 1431
Size: 1401 30
BssSI C'ACGA_G
Cuts at: 0 967 1431
Size: 967 464
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1356 1431
Size: 1356 75
BstYI r'GATC_y
Cuts at: 0 60 174 458 587 1412 1431
Size: 60 114 284 129 825 19
Fragments arranged by size:
825 284 129 114 60 19
BtgI C'CryG_G
Cuts at: 0 5 56 866 1431
Size: 5 51 810 565
Fragments arranged by size:
810 565 51 5
BtsI GCAGTG_nn'
Cuts at: 0 521 1431
Size: 521 910
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 24 59 218 253 1431
Size: 24 35 159 35 1178
Fragments arranged by size:
1178 159 35 35 24
EaeI y'GGCC_r
Cuts at: 0 830 1431
Size: 830 601
EarI CTCTTCn'nnn_
Cuts at: 0 293 1431
Size: 293 1138
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 564 598 1431
Size: 564 34 833
Fragments arranged by size:
833 564 34
EcoICRI GAG'CTC
Cuts at: 0 1254 1431
Size: 1254 177
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 111 576 1240 1270 1282 1291 1431
Size: 111 465 664 30 12 9 140
Fragments arranged by size:
664 465 140 111 30 12 9
EcoO109I rG'GnC_Cy
Cuts at: 0 636 1431
Size: 636 795
EcoRI G'AATT_C
Cuts at: 0 756 1431
Size: 756 675
EcoRV GAT'ATC
Cuts at: 0 99 600 1431
Size: 99 501 831
Fragments arranged by size:
831 501 99
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 417 1431
Size: 417 1014
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 232 264 1319 1351 1431
Size: 232 32 1055 32 80
Fragments arranged by size:
1055 232 80 32 32
HincII GTy'rAC
Cuts at: 0 12 120 1431
Size: 12 108 1311
Fragments arranged by size:
1311 108 12
HindIII A'AGCT_T
Cuts at: 0 350 424 1431
Size: 350 74 1007
Fragments arranged by size:
1007 350 74
MfeI C'AATT_G
Cuts at: 0 323 1431
Size: 323 1108
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 332 436 1431
Size: 332 104 995
Fragments arranged by size:
995 332 104
MscI TGG'CCA
Cuts at: 0 832 1431
Size: 832 599
MslI CAynn'nnrTG
Cuts at: 0 19 380 653 1431
Size: 19 361 273 778
Fragments arranged by size:
778 361 273 19
MspA1I CmG'CkG
Cuts at: 0 609 625 681 1260 1290 1431
Size: 609 16 56 579 30 141
Fragments arranged by size:
609 579 141 56 30 16
NcoI C'CATG_G
Cuts at: 0 5 1431
Size: 5 1426
PmlI CAC'GTG
Cuts at: 0 1347 1431
Size: 1347 84
PshAI GACnn'nnGTC
Cuts at: 0 1388 1431
Size: 1388 43
PspOMI G'GGCC_C
Cuts at: 0 635 1431
Size: 635 796
PstI C_TGCA'G
Cuts at: 0 812 1361 1409 1431
Size: 812 549 48 22
Fragments arranged by size:
812 549 48 22
PvuII CAG'CTG
Cuts at: 0 609 1260 1290 1431
Size: 609 651 30 141
Fragments arranged by size:
651 609 141 30
SacI G_AGCT'C
Cuts at: 0 1256 1431
Size: 1256 175
SapI GCTCTTCn'nnn_
Cuts at: 0 293 1431
Size: 293 1138
SbfI CC_TGCA'GG
Cuts at: 0 812 1409 1431
Size: 812 597 22
Fragments arranged by size:
812 597 22
ScaI AGT'ACT
Cuts at: 0 490 1431
Size: 490 941
SfcI C'TryA_G
Cuts at: 0 808 1357 1405 1431
Size: 808 549 48 26
Fragments arranged by size:
808 549 48 26
SspI AAT'ATT
Cuts at: 0 1157 1431
Size: 1157 274
StyI C'CwwG_G
Cuts at: 0 5 1032 1431
Size: 5 1027 399
Fragments arranged by size:
1027 399 5
TatI w'GTAC_w
Cuts at: 0 488 1431
Size: 488 943
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 73 105 1264 1296 1431
Size: 73 32 1159 32 135
Fragments arranged by size:
1159 135 73 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 315 1431
Size: 315 1116
XmnI GAAnn'nnTTC
Cuts at: 0 155 1223 1431
Size: 155 1068 208
Fragments arranged by size:
1068 208 155
Enzymes that do cut:
AarI AcuI AflIII AhdI AlwNI ApaI ApoI BamHI
BanII BbsI BclI BglI BglII Bme1580I BpmI BsaI
BsaAI BsaWI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BssSI BstAPI BstYI BtgI
BtsI CspCI EaeI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI Hin4I HincII HindIII MfeI MmeI
MscI MslI MspA1I NcoI PmlI PshAI PspOMI PstI
PvuII SacI SapI SbfI ScaI SfcI SspI StyI
TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AleI
AlfI AloI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BanI BbeI BbvCI BcgI BciVI BfrBI BlpI
BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaBI BsaHI
BseYI BsiEI BsiWI BsmBI BsrDI BsrFI BsrGI BssHII
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI DraI
DraIII DrdI EagI EcoNI FseI FspI FspAI HaeII
HpaI KasI KpnI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PsiI PspXI PsrI
PvuI RsrII SacII SalI SanDI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
StuI SwaI TaqII Tth111I XbaI XhoI XmaI ZraI