Restriction map for
pF1KB7759
(Linear) (Six-base) MAPSORT of: KIBB7759.seq Check: 2980 from: 1 to: 1341
KIBB7759.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 547 556 889 1341
Size: 203 344 9 333 452
Fragments arranged by size:
452 344 333 203 9
AfeI AGC'GCT
Cuts at: 0 757 1341
Size: 757 584
AleI CACnn'nnGTG
Cuts at: 0 1153 1341
Size: 1153 188
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 776 1292 1341
Size: 776 516 49
Fragments arranged by size:
776 516 49
AlwNI CAG_nnn'CTG
Cuts at: 0 110 1341
Size: 110 1231
ApaI G_GGCC'C
Cuts at: 0 21 1341
Size: 21 1320
ApaLI G'TGCA_C
Cuts at: 0 1333 1341
Size: 1333 8
ApoI r'AATT_y
Cuts at: 0 1000 1341
Size: 1000 341
AvaI C'yCGr_G
Cuts at: 0 315 1341
Size: 315 1026
BanI G'GyrC_C
Cuts at: 0 720 1341
Size: 720 621
BanII G_rGCy'C
Cuts at: 0 21 188 215 1341
Size: 21 167 27 1126
Fragments arranged by size:
1126 167 27 21
BbsI GAAGACnn'nnnn_
Cuts at: 0 877 977 1341
Size: 877 100 364
Fragments arranged by size:
877 364 100
BglI GCCn_nnn'nGGC
Cuts at: 0 407 1341
Size: 407 934
BglII A'GATC_T
Cuts at: 0 1322 1341
Size: 1322 19
BlpI GC'TnA_GC
Cuts at: 0 616 841 1341
Size: 616 225 500
Fragments arranged by size:
616 500 225
Bme1580I G_kGCm'C
Cuts at: 0 21 1337 1341
Size: 21 1316 4
Fragments arranged by size:
1316 21 4
BmrI ACTGGGnnnn_n'
Cuts at: 0 640 1341
Size: 640 701
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 919 1011 1111 1341
Size: 193 726 92 100 230
Fragments arranged by size:
726 230 193 100 92
Bpu10I CC'TnA_GC
Cuts at: 0 1249 1341
Size: 1249 92
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 931 1341
Size: 931 410
BsaWI w'CCGG_w
Cuts at: 0 1241 1341
Size: 1241 100
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 664 694 918 948 1341
Size: 664 30 224 30 393
Fragments arranged by size:
664 393 224 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 508 562 1341
Size: 508 54 779
Fragments arranged by size:
779 508 54
BseYI C'CCAG_C
Cuts at: 0 12 148 276 611 659 1341
Size: 12 136 128 335 48 682
Fragments arranged by size:
682 335 136 128 48 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 814 1135 1341
Size: 814 321 206
Fragments arranged by size:
814 321 206
BsiEI CG_ry'CG
Cuts at: 0 884 1341
Size: 884 457
BsiHKAI G_wGCw'C
Cuts at: 0 1337 1341
Size: 1337 4
BsmBI CGTCTCn'nnnn_
Cuts at: 0 792 1182 1341
Size: 792 390 159
Fragments arranged by size:
792 390 159
Bsp1286I G_dGCh'C
Cuts at: 0 21 188 215 1337 1341
Size: 21 167 27 1122 4
Fragments arranged by size:
1122 167 27 21 4
BspEI T'CCGG_A
Cuts at: 0 1241 1341
Size: 1241 100
BsrDI GCAATG_nn'
Cuts at: 0 1076 1341
Size: 1076 265
BsrFI r'CCGG_y
Cuts at: 0 674 692 1103 1341
Size: 674 18 411 238
Fragments arranged by size:
674 411 238 18
BstAPI GCAn_nnn'nTGC
Cuts at: 0 79 110 1341
Size: 79 31 1231
Fragments arranged by size:
1231 79 31
BstXI CCAn_nnnn'nTGG
Cuts at: 0 148 276 1341
Size: 148 128 1065
Fragments arranged by size:
1065 148 128
BstYI r'GATC_y
Cuts at: 0 233 518 726 1322 1341
Size: 233 285 208 596 19
Fragments arranged by size:
596 285 233 208 19
BtgI C'CryG_G
Cuts at: 0 422 716 1213 1341
Size: 422 294 497 128
Fragments arranged by size:
497 422 294 128
BtsI GCAGTG_nn'
Cuts at: 0 414 1279 1341
Size: 414 865 62
Fragments arranged by size:
865 414 62
EaeI y'GGCC_r
Cuts at: 0 411 779 1341
Size: 411 368 562
Fragments arranged by size:
562 411 368
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 355 511 1341
Size: 355 156 830
Fragments arranged by size:
830 355 156
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 203 547 556 889 919 1011 1111
Size: 193 10 344 9 333 30 92 100
Cuts at: 1111 1341
Size: 230
Fragments arranged by size:
344 333 230 193 100 92 30 10
9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 121 1341
Size: 121 1220
EcoO109I rG'GnC_Cy
Cuts at: 0 17 18 1341
Size: 17 1 1323
Fragments arranged by size:
1323 17 1
EcoRI G'AATT_C
Cuts at: 0 1000 1341
Size: 1000 341
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 378 1341
Size: 378 963
HaeII r_GCGC'y
Cuts at: 0 681 759 1341
Size: 681 78 582
Fragments arranged by size:
681 582 78
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 298 330 1341
Size: 298 32 1011
Fragments arranged by size:
1011 298 32
HincII GTy'rAC
Cuts at: 0 807 1341
Size: 807 534
MscI TGG'CCA
Cuts at: 0 781 1341
Size: 781 560
MslI CAynn'nnrTG
Cuts at: 0 329 433 1151 1153 1193 1341
Size: 329 104 718 2 40 148
Fragments arranged by size:
718 329 148 104 40 2
MspA1I CmG'CkG
Cuts at: 0 110 1311 1341
Size: 110 1201 30
Fragments arranged by size:
1201 110 30
NcoI C'CATG_G
Cuts at: 0 422 716 1341
Size: 422 294 625
Fragments arranged by size:
625 422 294
NdeI CA'TA_TG
Cuts at: 0 1152 1341
Size: 1152 189
NspI r_CATG'y
Cuts at: 0 438 489 1171 1341
Size: 438 51 682 170
Fragments arranged by size:
682 438 170 51
PasI CC'CwG_GG
Cuts at: 0 96 581 922 1341
Size: 96 485 341 419
Fragments arranged by size:
485 419 341 96
PsiI TTA'TAA
Cuts at: 0 962 1341
Size: 962 379
PspOMI G'GGCC_C
Cuts at: 0 17 1341
Size: 17 1324
PstI C_TGCA'G
Cuts at: 0 162 183 839 1101 1145 1292 1341
Size: 162 21 656 262 44 147 49
Fragments arranged by size:
656 262 162 147 49 44 21
PvuI CG_AT'CG
Cuts at: 0 884 1341
Size: 884 457
PvuII CAG'CTG
Cuts at: 0 110 1341
Size: 110 1231
SfcI C'TryA_G
Cuts at: 0 158 179 835 1097 1141 1288 1341
Size: 158 21 656 262 44 147 53
Fragments arranged by size:
656 262 158 147 53 44 21
SmlI C'TyrA_G
Cuts at: 0 946 1341
Size: 946 395
SphI G_CATG'C
Cuts at: 0 489 1171 1341
Size: 489 682 170
Fragments arranged by size:
682 489 170
StuI AGG'CCT
Cuts at: 0 313 1341
Size: 313 1028
StyI C'CwwG_G
Cuts at: 0 422 709 716 1341
Size: 422 287 7 625
Fragments arranged by size:
625 422 287 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1214 1341
Size: 1214 127
Enzymes that do cut:
AcuI AfeI AleI AlfI AlwNI ApaI ApaLI ApoI
AvaI BanI BanII BbsI BglI BglII BlpI Bme1580I
BmrI BpmI Bpu10I BpuEI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BsrDI BsrFI
BstAPI BstXI BstYI BtgI BtsI EaeI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII MscI
MslI MspA1I NcoI NdeI NspI PasI PsiI PspOMI
PstI PvuI PvuII SfcI SmlI SphI StuI StyI
TaqII
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AloI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbvCI BcgI BciVI BclI BfrBI BmgBI BmtI
BplI BsaI BsaAI BsaBI BsaHI BsiWI BsmI BspHI
BspMI BsrBI BsrGI BssHII BssSI BstBI BstEII BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI
EarI EcoICRI EcoRV FseI FspI FspAI HindIII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NgoMIV
NheI NotI NruI NsiI PacI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PspXI PsrI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI SwaI TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI