Restriction map for
pF1KB7754
(Linear) (Six-base) MAPSORT of: KIBB7754.seq Check: 1247 from: 1 to: 1488
KIBB7754.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AatII G_ACGT'C
Cuts at: 0 1247 1488
Size: 1247 241
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 123 464 694 1384 1413 1488
Size: 123 341 230 690 29 75
Fragments arranged by size:
690 341 230 123 75 29
AfeI AGC'GCT
Cuts at: 0 1174 1488
Size: 1174 314
AflIII A'CryG_T
Cuts at: 0 479 1076 1096 1235 1237 1488
Size: 479 597 20 139 2 251
Fragments arranged by size:
597 479 251 139 20 2
AhdI GACnn_n'nnGTC
Cuts at: 0 636 1488
Size: 636 852
AleI CACnn'nnGTG
Cuts at: 0 952 1488
Size: 952 536
AlwNI CAG_nnn'CTG
Cuts at: 0 183 249 555 1012 1488
Size: 183 66 306 457 476
Fragments arranged by size:
476 457 306 183 66
ApoI r'AATT_y
Cuts at: 0 990 1488
Size: 990 498
AvaI C'yCGr_G
Cuts at: 0 972 1488
Size: 972 516
BanII G_rGCy'C
Cuts at: 0 299 1021 1488
Size: 299 722 467
Fragments arranged by size:
722 467 299
BbsI GAAGACnn'nnnn_
Cuts at: 0 375 1488
Size: 375 1113
BbvCI CC'TCA_GC
Cuts at: 0 217 719 1488
Size: 217 502 769
Fragments arranged by size:
769 502 217
BglII A'GATC_T
Cuts at: 0 741 1488
Size: 741 747
BmgBI CAC'GTC
Cuts at: 0 111 480 1077 1488
Size: 111 369 597 411
Fragments arranged by size:
597 411 369 111
BmrI ACTGGGnnnn_n'
Cuts at: 0 1015 1488
Size: 1015 473
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 364 438 1488
Size: 283 81 74 1050
Fragments arranged by size:
1050 283 81 74
Bpu10I CC'TnA_GC
Cuts at: 0 217 719 1488
Size: 217 502 769
Fragments arranged by size:
769 502 217
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 920 1488
Size: 920 568
BsaI GGTCTCn'nnnn_
Cuts at: 0 639 1488
Size: 639 849
BsaAI yAC'GTr
Cuts at: 0 132 1097 1238 1488
Size: 132 965 141 250
Fragments arranged by size:
965 250 141 132
BsaHI Gr'CG_yC
Cuts at: 0 1244 1452 1488
Size: 1244 208 36
Fragments arranged by size:
1244 208 36
BsaWI w'CCGG_w
Cuts at: 0 1387 1488
Size: 1387 101
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 489 878 903 1488
Size: 489 389 25 585
Fragments arranged by size:
585 489 389 25
BseYI C'CCAG_C
Cuts at: 0 982 1488
Size: 982 506
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 439 1488
Size: 346 93 1049
Fragments arranged by size:
1049 346 93
BsiHKAI G_wGCw'C
Cuts at: 0 299 1373 1488
Size: 299 1074 115
Fragments arranged by size:
1074 299 115
BsmBI CGTCTCn'nnnn_
Cuts at: 0 860 1488
Size: 860 628
Bsp1286I G_dGCh'C
Cuts at: 0 299 1021 1373 1488
Size: 299 722 352 115
Fragments arranged by size:
722 352 299 115
BspEI T'CCGG_A
Cuts at: 0 1387 1488
Size: 1387 101
BspHI T'CATG_A
Cuts at: 0 275 311 1488
Size: 275 36 1177
Fragments arranged by size:
1177 275 36
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 359 1488
Size: 359 1129
BstAPI GCAn_nnn'nTGC
Cuts at: 0 555 1488
Size: 555 933
BstBI TT'CG_AA
Cuts at: 0 1319 1488
Size: 1319 169
BstEII G'GTnAC_C
Cuts at: 0 390 628 1488
Size: 390 238 860
Fragments arranged by size:
860 390 238
BstXI CCAn_nnnn'nTGG
Cuts at: 0 744 954 1488
Size: 744 210 534
Fragments arranged by size:
744 534 210
BstYI r'GATC_y
Cuts at: 0 245 551 669 741 1488
Size: 245 306 118 72 747
Fragments arranged by size:
747 306 245 118 72
BtgI C'CryG_G
Cuts at: 0 67 399 1488
Size: 67 332 1089
Fragments arranged by size:
1089 332 67
BtsI GCAGTG_nn'
Cuts at: 0 1054 1488
Size: 1054 434
DraIII CAC_nnn'GTG
Cuts at: 0 111 1488
Size: 111 1377
EaeI y'GGCC_r
Cuts at: 0 1038 1488
Size: 1038 450
EarI CTCTTCn'nnn_
Cuts at: 0 683 923 1081 1488
Size: 683 240 158 407
Fragments arranged by size:
683 407 240 158
EcoICRI GAG'CTC
Cuts at: 0 297 1488
Size: 297 1191
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 123 283 364 438 464 694 1384 1413
Size: 123 160 81 74 26 230 690 29
Cuts at: 1413 1488
Size: 75
Fragments arranged by size:
690 230 160 123 81 75 74 29
26
EcoO109I rG'GnC_Cy
Cuts at: 0 348 564 768 1009 1229 1488
Size: 348 216 204 241 220 259
Fragments arranged by size:
348 259 241 220 216 204
EcoRI G'AATT_C
Cuts at: 0 990 1488
Size: 990 498
EcoRV GAT'ATC
Cuts at: 0 432 1488
Size: 432 1056
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 675 1293 1488
Size: 675 618 195
Fragments arranged by size:
675 618 195
HaeII r_GCGC'y
Cuts at: 0 1176 1488
Size: 1176 312
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 622 654 898 930 1488
Size: 622 32 244 32 558
Fragments arranged by size:
622 558 244 32 32
HindIII A'AGCT_T
Cuts at: 0 839 1488
Size: 839 649
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 146 1488
Size: 38 108 1342
Fragments arranged by size:
1342 108 38
MscI TGG'CCA
Cuts at: 0 1040 1488
Size: 1040 448
MslI CAynn'nnrTG
Cuts at: 0 21 259 952 1034 1488
Size: 21 238 693 82 454
Fragments arranged by size:
693 454 238 82 21
MspA1I CmG'CkG
Cuts at: 0 152 854 1488
Size: 152 702 634
Fragments arranged by size:
702 634 152
NspI r_CATG'y
Cuts at: 0 258 1073 1488
Size: 258 815 415
Fragments arranged by size:
815 415 258
PasI CC'CwG_GG
Cuts at: 0 560 1488
Size: 560 928
PflMI CCAn_nnn'nTGG
Cuts at: 0 380 1488
Size: 380 1108
PfoI T'CCnGG_A
Cuts at: 0 248 577 1488
Size: 248 329 911
Fragments arranged by size:
911 329 248
PmlI CAC'GTG
Cuts at: 0 132 1238 1488
Size: 132 1106 250
Fragments arranged by size:
1106 250 132
PpuMI rG'GwC_Cy
Cuts at: 0 348 564 768 1488
Size: 348 216 204 720
Fragments arranged by size:
720 348 216 204
PstI C_TGCA'G
Cuts at: 0 551 1488
Size: 551 937
PvuII CAG'CTG
Cuts at: 0 152 1488
Size: 152 1336
SacI G_AGCT'C
Cuts at: 0 299 1488
Size: 299 1189
SanDI GG'GwC_CC
Cuts at: 0 564 1488
Size: 564 924
SapI GCTCTTCn'nnn_
Cuts at: 0 923 1488
Size: 923 565
SfcI C'TryA_G
Cuts at: 0 547 1350 1488
Size: 547 803 138
Fragments arranged by size:
803 547 138
SmaI CCC'GGG
Cuts at: 0 974 1488
Size: 974 514
SmlI C'TyrA_G
Cuts at: 0 935 1488
Size: 935 553
StyI C'CwwG_G
Cuts at: 0 659 1049 1488
Size: 659 390 439
Fragments arranged by size:
659 439 390
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1279 1488
Size: 1279 209
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 657 1488
Size: 657 831
XmaI C'CCGG_G
Cuts at: 0 972 1488
Size: 972 516
XmnI GAAnn'nnTTC
Cuts at: 0 1174 1258 1315 1342 1488
Size: 1174 84 57 27 146
Fragments arranged by size:
1174 146 84 57 27
ZraI GAC'GTC
Cuts at: 0 1245 1488
Size: 1245 243
Enzymes that do cut:
AatII AcuI AfeI AflIII AhdI AleI AlwNI ApoI
AvaI BanII BbsI BbvCI BglII BmgBI BmrI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BstAPI
BstBI BstEII BstXI BstYI BtgI BtsI DraIII EaeI
EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI HaeII
Hin4I HindIII MmeI MscI MslI MspA1I NspI PasI
PflMI PfoI PmlI PpuMI PstI PvuII SacI SanDI
SapI SfcI SmaI SmlI StyI TaqII XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflII AgeI AlfI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BanI BbeI BcgI BciVI BclI BfrBI BglI BlpI
Bme1580I BmtI BplI BsaBI BsaXI BsiEI BsiWI BsmI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DrdI EagI EciI EcoNI
FseI FspI FspAI HincII HpaI KasI KpnI MfeI
MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PmeI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI TstI Tth111I
XbaI XhoI