Restriction map for
pF1KB7748
(Linear) (Six-base) MAPSORT of: KIBB7748.seq Check: 291 from: 1 to: 1464
KIBB7748.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 24 1130 1290 1464
Size: 24 1106 160 174
Fragments arranged by size:
1106 174 160 24
AfeI AGC'GCT
Cuts at: 0 1419 1464
Size: 1419 45
AflIII A'CryG_T
Cuts at: 0 761 1464
Size: 761 703
AgeI A'CCGG_T
Cuts at: 0 610 1464
Size: 610 854
AhdI GACnn_n'nnGTC
Cuts at: 0 1143 1464
Size: 1143 321
AleI CACnn'nnGTG
Cuts at: 0 289 485 1464
Size: 289 196 979
Fragments arranged by size:
979 289 196
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1179 1211 1464
Size: 1179 32 253
Fragments arranged by size:
1179 253 32
AlwNI CAG_nnn'CTG
Cuts at: 0 47 333 1464
Size: 47 286 1131
Fragments arranged by size:
1131 286 47
ApaLI G'TGCA_C
Cuts at: 0 631 1464
Size: 631 833
ApoI r'AATT_y
Cuts at: 0 523 553 735 1464
Size: 523 30 182 729
Fragments arranged by size:
729 523 182 30
AvaI C'yCGr_G
Cuts at: 0 425 453 1432 1464
Size: 425 28 979 32
Fragments arranged by size:
979 425 32 28
BanI G'GyrC_C
Cuts at: 0 297 433 1237 1375 1464
Size: 297 136 804 138 89
Fragments arranged by size:
804 297 138 136 89
BanII G_rGCy'C
Cuts at: 0 232 1065 1099 1464
Size: 232 833 34 365
Fragments arranged by size:
833 365 232 34
BbeI G_GCGC'C
Cuts at: 0 301 437 1241 1379 1464
Size: 301 136 804 138 85
Fragments arranged by size:
804 301 138 136 85
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 182 216 682 716 1464
Size: 182 34 466 34 748
Fragments arranged by size:
748 466 182 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 16 83 1367 1464
Size: 16 67 1284 97
Fragments arranged by size:
1284 97 67 16
BglII A'GATC_T
Cuts at: 0 591 1464
Size: 591 873
Bme1580I G_kGCm'C
Cuts at: 0 635 1464
Size: 635 829
BmrI ACTGGGnnnn_n'
Cuts at: 0 283 1464
Size: 283 1181
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 492 892 1464
Size: 492 400 572
Fragments arranged by size:
572 492 400
BsaAI yAC'GTr
Cuts at: 0 900 1464
Size: 900 564
BsaHI Gr'CG_yC
Cuts at: 0 298 434 1238 1376 1464
Size: 298 136 804 138 88
Fragments arranged by size:
804 298 138 136 88
BsaWI w'CCGG_w
Cuts at: 0 610 1464
Size: 610 854
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 865 895 1179 1209 1464
Size: 865 30 284 30 255
Fragments arranged by size:
865 284 255 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 57 1107 1464
Size: 57 1050 357
Fragments arranged by size:
1050 357 57
BseYI C'CCAG_C
Cuts at: 0 936 1099 1162 1261 1464
Size: 936 163 63 99 203
Fragments arranged by size:
936 203 163 99 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 1186 1464
Size: 133 1053 278
Fragments arranged by size:
1053 278 133
BsiEI CG_ry'CG
Cuts at: 0 56 74 206 659 1464
Size: 56 18 132 453 805
Fragments arranged by size:
805 453 132 56 18
BsiHKAI G_wGCw'C
Cuts at: 0 635 788 1464
Size: 635 153 676
Fragments arranged by size:
676 635 153
Bsp1286I G_dGCh'C
Cuts at: 0 232 635 788 1065 1099 1464
Size: 232 403 153 277 34 365
Fragments arranged by size:
403 365 277 232 153 34
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 863 1464
Size: 863 601
BsrBI CCG'CTC
Cuts at: 0 342 1176 1464
Size: 342 834 288
Fragments arranged by size:
834 342 288
BsrFI r'CCGG_y
Cuts at: 0 73 154 610 658 977 1464
Size: 73 81 456 48 319 487
Fragments arranged by size:
487 456 319 81 73 48
BssHII G'CGCG_C
Cuts at: 0 837 1448 1464
Size: 837 611 16
Fragments arranged by size:
837 611 16
BstXI CCAn_nnnn'nTGG
Cuts at: 0 291 487 1464
Size: 291 196 977
Fragments arranged by size:
977 291 196
BstYI r'GATC_y
Cuts at: 0 165 268 591 681 1464
Size: 165 103 323 90 783
Fragments arranged by size:
783 323 165 103 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 120 946 1464
Size: 120 826 518
Fragments arranged by size:
826 518 120
BtsI GCAGTG_nn'
Cuts at: 0 1263 1464
Size: 1263 201
DrdI GACnn_nn'nnGTC
Cuts at: 0 565 1464
Size: 565 899
EaeI y'GGCC_r
Cuts at: 0 53 71 203 263 656 660 1464
Size: 53 18 132 60 393 4 804
Fragments arranged by size:
804 393 132 60 53 18 4
EagI C'GGCC_G
Cuts at: 0 53 71 203 656 1464
Size: 53 18 132 453 808
Fragments arranged by size:
808 453 132 53 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 404 410 996 1464
Size: 404 6 586 468
Fragments arranged by size:
586 468 404 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 24 492 892 1130 1290 1464
Size: 24 468 400 238 160 174
Fragments arranged by size:
468 400 238 174 160 24
EcoO109I rG'GnC_Cy
Cuts at: 0 186 197 772 1224 1464
Size: 186 11 575 452 240
Fragments arranged by size:
575 452 240 186 11
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 144 1464
Size: 144 1320
FseI GG_CCGG'CC
Cuts at: 0 77 662 1464
Size: 77 585 802
Fragments arranged by size:
802 585 77
HaeII r_GCGC'y
Cuts at: 0 301 437 1241 1316 1379 1421 1464
Size: 301 136 804 75 63 42 43
Fragments arranged by size:
804 301 136 75 63 43 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1036 1068 1129 1161 1464
Size: 1036 32 61 32 303
Fragments arranged by size:
1036 303 61 32 32
KasI G'GCGC_C
Cuts at: 0 297 433 1237 1375 1464
Size: 297 136 804 138 89
Fragments arranged by size:
804 297 138 136 89
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 875 1464
Size: 59 816 589
Fragments arranged by size:
816 589 59
MslI CAynn'nnrTG
Cuts at: 0 289 485 1464
Size: 289 196 979
Fragments arranged by size:
979 289 196
MspA1I CmG'CkG
Cuts at: 0 330 924 1280 1464
Size: 330 594 356 184
Fragments arranged by size:
594 356 330 184
NaeI GCC'GGC
Cuts at: 0 75 156 660 1464
Size: 75 81 504 804
Fragments arranged by size:
804 504 81 75
NarI GG'CG_CC
Cuts at: 0 298 434 1238 1376 1464
Size: 298 136 804 138 88
Fragments arranged by size:
804 298 138 136 88
NgoMIV G'CCGG_C
Cuts at: 0 73 154 658 1464
Size: 73 81 504 806
Fragments arranged by size:
806 504 81 73
PflMI CCAn_nnn'nTGG
Cuts at: 0 1057 1464
Size: 1057 407
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1179 1211 1464
Size: 1179 32 253
Fragments arranged by size:
1179 253 32
PpuMI rG'GwC_Cy
Cuts at: 0 772 1464
Size: 772 692
PstI C_TGCA'G
Cuts at: 0 443 552 650 860 1464
Size: 443 109 98 210 604
Fragments arranged by size:
604 443 210 109 98
PvuII CAG'CTG
Cuts at: 0 924 1464
Size: 924 540
RsrII CG'GwC_CG
Cuts at: 0 912 1464
Size: 912 552
SfcI C'TryA_G
Cuts at: 0 439 548 646 856 1464
Size: 439 109 98 210 608
Fragments arranged by size:
608 439 210 109 98
SfoI GGC'GCC
Cuts at: 0 299 435 1239 1377 1464
Size: 299 136 804 138 87
Fragments arranged by size:
804 299 138 136 87
SgrAI Cr'CCGG_yG
Cuts at: 0 154 1464
Size: 154 1310
SmaI CCC'GGG
Cuts at: 0 427 1464
Size: 427 1037
StuI AGG'CCT
Cuts at: 0 644 869 1464
Size: 644 225 595
Fragments arranged by size:
644 595 225
StyI C'CwwG_G
Cuts at: 0 98 1001 1362 1443 1464
Size: 98 903 361 81 21
Fragments arranged by size:
903 361 98 81 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 929 1464
Size: 929 535
XbaI T'CTAG_A
Cuts at: 0 678 1464
Size: 678 786
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 291 1450 1464
Size: 291 1159 14
Fragments arranged by size:
1159 291 14
XmaI C'CCGG_G
Cuts at: 0 425 1464
Size: 425 1039
Enzymes that do cut:
AcuI AfeI AflIII AgeI AhdI AleI AloI AlwNI
ApaLI ApoI AvaI BanI BanII BbeI BcgI BglI
BglII Bme1580I BmrI BpmI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI
BsrFI BssHII BstXI BstYI BtgZI BtsI DrdI EaeI
EagI EciI Eco57MI EcoO109I FalI FseI HaeII Hin4I
KasI MmeI MslI MspA1I NaeI NarI NgoMIV PflMI
PpiI PpuMI PstI PvuII RsrII SfcI SfoI SgrAI
SmaI StuI StyI TaqII XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AlfI ApaI
AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI
BciVI BclI BfrBI BlpI BmgBI BmtI BplI Bpu10I
BpuEI BsaI BsaBI BsiWI BsmI BsmBI BspEI BspHI
BsrDI BsrGI BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
BtgI ClaI CspCI DraI DraIII EarI EcoICRI EcoNI
EcoRI EcoRV FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI MscI NcoI NdeI NheI NotI NruI
NsiI NspI PacI PasI PciI PfoI PmeI PmlI
PshAI PsiI PspOMI PspXI PsrI PvuI SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI
SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI
TstI Tth111I XhoI XmnI ZraI