Restriction map for
pF1KB7742
(Linear) (Six-base) MAPSORT of: KIBB7742.seq Check: 4908 from: 1 to: 1458
KIBB7742.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 768 1458
Size: 768 690
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 733 1229 1458
Size: 733 496 229
Fragments arranged by size:
733 496 229
AfeI AGC'GCT
Cuts at: 0 624 1458
Size: 624 834
AlwNI CAG_nnn'CTG
Cuts at: 0 73 940 1090 1458
Size: 73 867 150 368
Fragments arranged by size:
867 368 150 73
ApaI G_GGCC'C
Cuts at: 0 230 1458
Size: 230 1228
ApoI r'AATT_y
Cuts at: 0 721 1458
Size: 721 737
AvaI C'yCGr_G
Cuts at: 0 1102 1114 1201 1458
Size: 1102 12 87 257
Fragments arranged by size:
1102 257 87 12
AvrII C'CTAG_G
Cuts at: 0 1036 1458
Size: 1036 422
BanI G'GyrC_C
Cuts at: 0 700 1458
Size: 700 758
BanII G_rGCy'C
Cuts at: 0 230 826 1022 1103 1115 1458
Size: 230 596 196 81 12 343
Fragments arranged by size:
596 343 230 196 81 12
BbsI GAAGACnn'nnnn_
Cuts at: 0 676 1458
Size: 676 782
BbvCI CC'TCA_GC
Cuts at: 0 169 737 1458
Size: 169 568 721
Fragments arranged by size:
721 568 169
BglI GCCn_nnn'nGGC
Cuts at: 0 990 1458
Size: 990 468
Bme1580I G_kGCm'C
Cuts at: 0 230 705 977 1458
Size: 230 475 272 481
Fragments arranged by size:
481 475 272 230
BmrI ACTGGGnnnn_n'
Cuts at: 0 964 1458
Size: 964 494
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 345 1458
Size: 345 1113
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 896 1039 1458
Size: 896 143 419
Fragments arranged by size:
896 419 143
Bpu10I CC'TnA_GC
Cuts at: 0 169 737 1091 1097 1109 1458
Size: 169 568 354 6 12 349
Fragments arranged by size:
568 354 349 169 12 6
BsaI GGTCTCn'nnnn_
Cuts at: 0 37 266 1458
Size: 37 229 1192
Fragments arranged by size:
1192 229 37
BsaHI Gr'CG_yC
Cuts at: 0 676 1458
Size: 676 782
BsaWI w'CCGG_w
Cuts at: 0 1350 1458
Size: 1350 108
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 202 754 778 834 885 914 1030 1159
Size: 202 552 24 56 51 29 116 129
Cuts at: 1159 1207 1458
Size: 48 251
Fragments arranged by size:
552 251 202 129 116 56 51 48
29 24
BseYI C'CCAG_C
Cuts at: 0 1023 1258 1264 1270 1458
Size: 1023 235 6 6 188
Fragments arranged by size:
1023 235 188 6 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 311 1458
Size: 311 1147
BsiHKAI G_wGCw'C
Cuts at: 0 1202 1458
Size: 1202 256
BsmBI CGTCTCn'nnnn_
Cuts at: 0 532 1458
Size: 532 926
Bsp1286I G_dGCh'C
Cuts at: 0 230 705 826 977 1022 1103 1115 1202
Size: 230 475 121 151 45 81 12 87
Cuts at: 1202 1458
Size: 256
Fragments arranged by size:
475 256 230 151 121 87 81 45
12
BspEI T'CCGG_A
Cuts at: 0 1350 1458
Size: 1350 108
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 768 1458
Size: 768 690
BsrFI r'CCGG_y
Cuts at: 0 476 870 1458
Size: 476 394 588
Fragments arranged by size:
588 476 394
BssSI C'ACGA_G
Cuts at: 0 461 550 1458
Size: 461 89 908
Fragments arranged by size:
908 461 89
BstXI CCAn_nnnn'nTGG
Cuts at: 0 247 1458
Size: 247 1211
BstYI r'GATC_y
Cuts at: 0 69 1289 1458
Size: 69 1220 169
Fragments arranged by size:
1220 169 69
Bsu36I CC'TnA_GG
Cuts at: 0 827 1458
Size: 827 631
BtgI C'CryG_G
Cuts at: 0 38 240 705 731 1410 1458
Size: 38 202 465 26 679 48
Fragments arranged by size:
679 465 202 48 38 26
BtsI GCAGTG_nn'
Cuts at: 0 299 877 1458
Size: 299 578 581
Fragments arranged by size:
581 578 299
DraIII CAC_nnn'GTG
Cuts at: 0 972 1458
Size: 972 486
EaeI y'GGCC_r
Cuts at: 0 474 585 782 1413 1458
Size: 474 111 197 631 45
Fragments arranged by size:
631 474 197 111 45
EarI CTCTTCn'nnn_
Cuts at: 0 336 739 755 851 1061 1432 1458
Size: 336 403 16 96 210 371 26
Fragments arranged by size:
403 371 336 210 96 26 16
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 733 896 1039 1229 1458
Size: 733 163 143 190 229
Fragments arranged by size:
733 229 190 163 143
EcoO109I rG'GnC_Cy
Cuts at: 0 226 648 1458
Size: 226 422 810
Fragments arranged by size:
810 422 226
HaeII r_GCGC'y
Cuts at: 0 626 1458
Size: 626 832
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 205 237 1458
Size: 205 32 1221
Fragments arranged by size:
1221 205 32
HindIII A'AGCT_T
Cuts at: 0 1447 1458
Size: 1447 11
MscI TGG'CCA
Cuts at: 0 587 784 1415 1458
Size: 587 197 631 43
Fragments arranged by size:
631 587 197 43
MslI CAynn'nnrTG
Cuts at: 0 245 305 1375 1458
Size: 245 60 1070 83
Fragments arranged by size:
1070 245 83 60
MspA1I CmG'CkG
Cuts at: 0 168 1297 1458
Size: 168 1129 161
Fragments arranged by size:
1129 168 161
NcoI C'CATG_G
Cuts at: 0 240 731 1410 1458
Size: 240 491 679 48
Fragments arranged by size:
679 491 240 48
PasI CC'CwG_GG
Cuts at: 0 48 1458
Size: 48 1410
PfoI T'CCnGG_A
Cuts at: 0 1001 1458
Size: 1001 457
PsiI TTA'TAA
Cuts at: 0 419 665 1458
Size: 419 246 793
Fragments arranged by size:
793 419 246
PspOMI G'GGCC_C
Cuts at: 0 226 1458
Size: 226 1232
PspXI vC'TCGA_Gb
Cuts at: 0 1201 1458
Size: 1201 257
PvuII CAG'CTG
Cuts at: 0 168 1297 1458
Size: 168 1129 161
Fragments arranged by size:
1129 168 161
SapI GCTCTTCn'nnn_
Cuts at: 0 851 1061 1458
Size: 851 210 397
Fragments arranged by size:
851 397 210
SfcI C'TryA_G
Cuts at: 0 11 1458
Size: 11 1447
SmlI C'TyrA_G
Cuts at: 0 1201 1458
Size: 1201 257
StuI AGG'CCT
Cuts at: 0 904 1052 1458
Size: 904 148 406
Fragments arranged by size:
904 406 148
StyI C'CwwG_G
Cuts at: 0 240 572 731 1036 1317 1410 1458
Size: 240 332 159 305 281 93 48
Fragments arranged by size:
332 305 281 240 159 93 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 290 623 983 1458
Size: 290 333 360 475
Fragments arranged by size:
475 360 333 290
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1417 1458
Size: 1417 41
XhoI C'TCGA_G
Cuts at: 0 1201 1458
Size: 1201 257
Enzymes that do cut:
AarI AcuI AfeI AlwNI ApaI ApoI AvaI AvrII
BanI BanII BbsI BbvCI BglI Bme1580I BmrI BplI
BpmI Bpu10I BsaI BsaHI BsaWI BseRI BseYI BsgI
BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrFI BssSI BstXI
BstYI Bsu36I BtgI BtsI DraIII EaeI EarI Eco57MI
EcoO109I HaeII Hin4I HindIII MscI MslI MspA1I NcoI
PasI PfoI PsiI PspOMI PspXI PvuII SapI SfcI
SmlI StuI StyI TaqII XcmI XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI BaeI
BamHI BbeI BcgI BciVI BclI BfrBI BglII BlpI
BmgBI BmtI BpuEI BsaAI BsaBI BsaXI BsiEI BsiWI
BsmI BspHI BsrBI BsrDI BsrGI BssHII BstAPI BstBI
BstEII BstZ17I BtgZI ClaI CspCI DraI DrdI EagI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HincII HpaI KasI KpnI MfeI MluI MmeI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI TatI
TstI Tth111I XbaI XmaI XmnI ZraI