Restriction map for
pF1KB7740
(Linear) (Six-base) MAPSORT of: KIBB7740.seq Check: 2877 from: 1 to: 1446
KIBB7740.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AccI GT'mk_AC
Cuts at: 0 576 1446
Size: 576 870
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 465 1234 1446
Size: 281 184 769 212
Fragments arranged by size:
769 281 212 184
AlwNI CAG_nnn'CTG
Cuts at: 0 150 1446
Size: 150 1296
ApoI r'AATT_y
Cuts at: 0 629 845 859 876 928 1082 1446
Size: 629 216 14 17 52 154 364
Fragments arranged by size:
629 364 216 154 52 17 14
BbsI GAAGACnn'nnnn_
Cuts at: 0 701 977 1446
Size: 701 276 469
Fragments arranged by size:
701 469 276
BciVI GTATCCnnnnn_n'
Cuts at: 0 430 1446
Size: 430 1016
BmgBI CAC'GTC
Cuts at: 0 1440 1446
Size: 1440 6
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 288 842 1446
Size: 288 554 604
Fragments arranged by size:
604 554 288
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1190 1446
Size: 1190 256
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1148 1446
Size: 1148 298
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1183 1446
Size: 1183 263
BseYI C'CCAG_C
Cuts at: 0 251 1446
Size: 251 1195
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 887 1446
Size: 887 559
BsmI GAATG_Cn'
Cuts at: 0 619 1059 1446
Size: 619 440 387
Fragments arranged by size:
619 440 387
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 121 1446
Size: 121 1325
BsrDI GCAATG_nn'
Cuts at: 0 186 1446
Size: 186 1260
BsrGI T'GTAC_A
Cuts at: 0 509 548 912 1446
Size: 509 39 364 534
Fragments arranged by size:
534 509 364 39
BstBI TT'CG_AA
Cuts at: 0 1086 1446
Size: 1086 360
Bsu36I CC'TnA_GG
Cuts at: 0 827 1446
Size: 827 619
BtsI GCAGTG_nn'
Cuts at: 0 48 1446
Size: 48 1398
DraI TTT'AAA
Cuts at: 0 851 1446
Size: 851 595
DrdI GACnn_nn'nnGTC
Cuts at: 0 738 1446
Size: 738 708
EarI CTCTTCn'nnn_
Cuts at: 0 257 788 1446
Size: 257 531 658
Fragments arranged by size:
658 531 257
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 465 1190 1234 1446
Size: 281 184 725 44 212
Fragments arranged by size:
725 281 212 184 44
EcoO109I rG'GnC_Cy
Cuts at: 0 133 1446
Size: 133 1313
EcoRI G'AATT_C
Cuts at: 0 859 928 1082 1446
Size: 859 69 154 364
Fragments arranged by size:
859 364 154 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 415 447 1344 1376 1446
Size: 415 32 897 32 70
Fragments arranged by size:
897 415 70 32 32
HincII GTy'rAC
Cuts at: 0 577 623 1446
Size: 577 46 823
Fragments arranged by size:
823 577 46
HindIII A'AGCT_T
Cuts at: 0 1246 1446
Size: 1246 200
HpaI GTT'AAC
Cuts at: 0 623 1446
Size: 623 823
MfeI C'AATT_G
Cuts at: 0 1190 1446
Size: 1190 256
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 257 1446
Size: 257 1189
MslI CAynn'nnrTG
Cuts at: 0 82 446 1446
Size: 82 364 1000
Fragments arranged by size:
1000 364 82
MspA1I CmG'CkG
Cuts at: 0 56 307 1027 1446
Size: 56 251 720 419
Fragments arranged by size:
720 419 251 56
NspI r_CATG'y
Cuts at: 0 555 676 1446
Size: 555 121 770
Fragments arranged by size:
770 555 121
PfoI T'CCnGG_A
Cuts at: 0 276 1446
Size: 276 1170
PpuMI rG'GwC_Cy
Cuts at: 0 133 1446
Size: 133 1313
PsiI TTA'TAA
Cuts at: 0 1136 1446
Size: 1136 310
PstI C_TGCA'G
Cuts at: 0 55 1446
Size: 55 1391
PvuII CAG'CTG
Cuts at: 0 56 307 1027 1446
Size: 56 251 720 419
Fragments arranged by size:
720 419 251 56
SalI G'TCGA_C
Cuts at: 0 575 1446
Size: 575 871
SfcI C'TryA_G
Cuts at: 0 51 1446
Size: 51 1395
SmlI C'TyrA_G
Cuts at: 0 1163 1446
Size: 1163 283
SphI G_CATG'C
Cuts at: 0 676 1446
Size: 676 770
TatI w'GTAC_w
Cuts at: 0 509 548 912 1446
Size: 509 39 364 534
Fragments arranged by size:
534 509 364 39
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 391 423 1446
Size: 391 32 1023
Fragments arranged by size:
1023 391 32
XmnI GAAnn'nnTTC
Cuts at: 0 878 1082 1446
Size: 878 204 364
Fragments arranged by size:
878 364 204
Enzymes that do cut:
AccI AcuI AlwNI ApoI BbsI BciVI BmgBI BplI
BpmI BpuEI BseRI BseYI BsgI BsmI BspMI BsrDI
BsrGI BstBI Bsu36I BtsI DraI DrdI EarI Eco57MI
EcoO109I EcoRI Hin4I HincII HindIII HpaI MfeI MmeI
MslI MspA1I NspI PfoI PpuMI PsiI PstI PvuII
SalI SfcI SmlI SphI TatI TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbvCI BcgI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BmtI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI
BsrBI BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI
BstZ17I BtgI BtgZI ClaI CspCI DraIII EaeI EagI
EciI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI
HaeII KasI KpnI MluI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PflMI PmeI PmlI PpiI PshAI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI StuI StyI SwaI TaqII Tth111I
XbaI XcmI XhoI XmaI ZraI