Restriction map for
pF1KB7737
(Linear) (Six-base) MAPSORT of: KIBB7737.seq Check: 8057 from: 1 to: 1308
KIBB7737.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AccI GT'mk_AC
Cuts at: 0 515 1308
Size: 515 793
Acc65I G'GTAC_C
Cuts at: 0 1190 1212 1308
Size: 1190 22 96
Fragments arranged by size:
1190 96 22
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 799 1308
Size: 799 509
AleI CACnn'nnGTG
Cuts at: 0 1017 1308
Size: 1017 291
AlwNI CAG_nnn'CTG
Cuts at: 0 385 830 1308
Size: 385 445 478
Fragments arranged by size:
478 445 385
ApaI G_GGCC'C
Cuts at: 0 56 877 1077 1246 1308
Size: 56 821 200 169 62
Fragments arranged by size:
821 200 169 62 56
ApaLI G'TGCA_C
Cuts at: 0 545 1308
Size: 545 763
AvaI C'yCGr_G
Cuts at: 0 264 397 724 988 1308
Size: 264 133 327 264 320
Fragments arranged by size:
327 320 264 264 133
BanI G'GyrC_C
Cuts at: 0 1011 1190 1212 1308
Size: 1011 179 22 96
Fragments arranged by size:
1011 179 96 22
BanII G_rGCy'C
Cuts at: 0 56 447 479 497 877 1077 1102 1186
Size: 56 391 32 18 380 200 25 84
Cuts at: 1186 1246 1294 1308
Size: 60 48 14
Fragments arranged by size:
391 380 200 84 60 56 48 32
25 18 14
BbvCI CC'TCA_GC
Cuts at: 0 1037 1308
Size: 1037 271
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 517 551 1308
Size: 517 34 757
Fragments arranged by size:
757 517 34
BclI T'GATC_A
Cuts at: 0 332 1308
Size: 332 976
BglI GCCn_nnn'nGGC
Cuts at: 0 895 1308
Size: 895 413
BlpI GC'TnA_GC
Cuts at: 0 1134 1308
Size: 1134 174
Bme1580I G_kGCm'C
Cuts at: 0 56 223 549 877 1077 1246 1308
Size: 56 167 326 328 200 169 62
Fragments arranged by size:
328 326 200 169 167 62 56
BmrI ACTGGGnnnn_n'
Cuts at: 0 705 1025 1073 1308
Size: 705 320 48 235
Fragments arranged by size:
705 320 235 48
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 330 1308
Size: 330 978
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 227 304 1103 1180 1308
Size: 60 167 77 799 77 128
Fragments arranged by size:
799 167 128 77 77 60
Bpu10I CC'TnA_GC
Cuts at: 0 277 1037 1308
Size: 277 760 271
Fragments arranged by size:
760 277 271
BsaI GGTCTCn'nnnn_
Cuts at: 0 721 1308
Size: 721 587
BsaHI Gr'CG_yC
Cuts at: 0 848 1308
Size: 848 460
BsaWI w'CCGG_w
Cuts at: 0 432 1308
Size: 432 876
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1217 1247 1308
Size: 1217 30 61
Fragments arranged by size:
1217 61 30
BseYI C'CCAG_C
Cuts at: 0 346 470 478 677 981 1102 1194 1308
Size: 346 124 8 199 304 121 92 114
Fragments arranged by size:
346 304 199 124 121 114 92 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 613 1036 1308
Size: 613 423 272
Fragments arranged by size:
613 423 272
BsiHKAI G_wGCw'C
Cuts at: 0 549 1308
Size: 549 759
BsmBI CGTCTCn'nnnn_
Cuts at: 0 839 1308
Size: 839 469
Bsp1286I G_dGCh'C
Cuts at: 0 56 223 447 479 497 549 877 1077
Size: 56 167 224 32 18 52 328 200
Cuts at: 1077 1102 1186 1246 1294 1308
Size: 25 84 60 48 14
Fragments arranged by size:
328 224 200 167 84 60 56 52
48 32 25 18 14
BsrBI CCG'CTC
Cuts at: 0 908 1308
Size: 908 400
BsrFI r'CCGG_y
Cuts at: 0 37 228 464 1206 1308
Size: 37 191 236 742 102
Fragments arranged by size:
742 236 191 102 37
BssSI C'ACGA_G
Cuts at: 0 11 1308
Size: 11 1297
BstAPI GCAn_nnn'nTGC
Cuts at: 0 636 1308
Size: 636 672
BstYI r'GATC_y
Cuts at: 0 966 1308
Size: 966 342
BtgI C'CryG_G
Cuts at: 0 215 612 1068 1308
Size: 215 397 456 240
Fragments arranged by size:
456 397 240 215
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 34 1308
Size: 34 1274
DraIII CAC_nnn'GTG
Cuts at: 0 922 1308
Size: 922 386
EaeI y'GGCC_r
Cuts at: 0 179 615 1308
Size: 179 436 693
Fragments arranged by size:
693 436 179
EarI CTCTTCn'nnn_
Cuts at: 0 853 1308
Size: 853 455
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 227 304 799 1103 1180 1308
Size: 60 167 77 495 304 77 128
Fragments arranged by size:
495 304 167 128 77 77 60
EcoNI CCTnn'n_nnAGG
Cuts at: 0 97 1308
Size: 97 1211
EcoO109I rG'GnC_Cy
Cuts at: 0 52 249 873 978 1073 1074 1242 1243
Size: 52 197 624 105 95 1 168 1
Cuts at: 1243 1308
Size: 65
Fragments arranged by size:
624 197 168 105 95 65 52 1
1
HaeII r_GCGC'y
Cuts at: 0 471 1134 1308
Size: 471 663 174
Fragments arranged by size:
663 471 174
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 365 397 1276 1308
Size: 365 32 879 32
Fragments arranged by size:
879 365 32 32
KpnI G_GTAC'C
Cuts at: 0 1194 1216 1308
Size: 1194 22 92
Fragments arranged by size:
1194 92 22
MslI CAynn'nnrTG
Cuts at: 0 742 1017 1308
Size: 742 275 291
Fragments arranged by size:
742 291 275
MspA1I CmG'CkG
Cuts at: 0 455 482 1003 1198 1308
Size: 455 27 521 195 110
Fragments arranged by size:
521 455 195 110 27
NaeI GCC'GGC
Cuts at: 0 39 230 466 1308
Size: 39 191 236 842
Fragments arranged by size:
842 236 191 39
NcoI C'CATG_G
Cuts at: 0 215 1068 1308
Size: 215 853 240
Fragments arranged by size:
853 240 215
NgoMIV G'CCGG_C
Cuts at: 0 37 228 464 1308
Size: 37 191 236 844
Fragments arranged by size:
844 236 191 37
NspI r_CATG'y
Cuts at: 0 360 1308
Size: 360 948
PasI CC'CwG_GG
Cuts at: 0 30 942 1308
Size: 30 912 366
Fragments arranged by size:
912 366 30
PfoI T'CCnGG_A
Cuts at: 0 151 812 1109 1308
Size: 151 661 297 199
Fragments arranged by size:
661 297 199 151
PspOMI G'GGCC_C
Cuts at: 0 52 873 1073 1242 1308
Size: 52 821 200 169 66
Fragments arranged by size:
821 200 169 66 52
PstI C_TGCA'G
Cuts at: 0 688 1308
Size: 688 620
SexAI A'CCwGG_T
Cuts at: 0 918 1308
Size: 918 390
SfcI C'TryA_G
Cuts at: 0 684 1308
Size: 684 624
SgrAI Cr'CCGG_yG
Cuts at: 0 1206 1308
Size: 1206 102
SmaI CCC'GGG
Cuts at: 0 266 990 1308
Size: 266 724 318
Fragments arranged by size:
724 318 266
StuI AGG'CCT
Cuts at: 0 898 1042 1308
Size: 898 144 266
Fragments arranged by size:
898 266 144
StyI C'CwwG_G
Cuts at: 0 215 1068 1236 1308
Size: 215 853 168 72
Fragments arranged by size:
853 215 168 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 203 934 1308
Size: 203 731 374
Fragments arranged by size:
731 374 203
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1217 1249 1308
Size: 1217 32 59
Fragments arranged by size:
1217 59 32
Tth111I GACn'n_nGTC
Cuts at: 0 511 1308
Size: 511 797
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 600 1308
Size: 600 708
XmaI C'CCGG_G
Cuts at: 0 264 988 1308
Size: 264 724 320
Fragments arranged by size:
724 320 264
XmnI GAAnn'nnTTC
Cuts at: 0 307 1308
Size: 307 1001
Enzymes that do cut:
AccI Acc65I AcuI AleI AlwNI ApaI ApaLI AvaI
BanI BanII BbvCI BcgI BclI BglI BlpI Bme1580I
BmrI BplI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BsrBI BsrFI BssSI
BstAPI BstYI BtgI CspCI DraIII EaeI EarI Eco57MI
EcoNI EcoO109I HaeII Hin4I KpnI MslI MspA1I NaeI
NcoI NgoMIV NspI PasI PfoI PspOMI PstI SexAI
SfcI SgrAI SmaI StuI StyI TaqII TstI Tth111I
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbsI BciVI BfrBI BglII BmgBI BmtI
BpuEI BsaAI BsaBI BseRI BsiEI BsiWI BsmI BspEI
BspHI BspMI BsrDI BsrGI BssHII BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI BtsI ClaI DraI DrdI EagI
EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KasI MfeI MluI MmeI MscI
NarI NdeI NheI NotI NruI NsiI PacI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TatI XbaI XhoI
ZraI