Restriction map for
pF1KB7710
(Linear) (Six-base) MAPSORT of: KIBB7710.seq Check: 9896 from: 1 to: 1395
KIBB7710.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 719 1263 1395
Size: 719 544 132
Fragments arranged by size:
719 544 132
AccI GT'mk_AC
Cuts at: 0 396 1395
Size: 396 999
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 1395
Size: 40 1355
AhdI GACnn_n'nnGTC
Cuts at: 0 939 1395
Size: 939 456
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 178 1231 1395
Size: 178 1053 164
Fragments arranged by size:
1053 178 164
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1356 1388 1395
Size: 1356 32 7
Fragments arranged by size:
1356 32 7
AlwNI CAG_nnn'CTG
Cuts at: 0 537 1395
Size: 537 858
ApaI G_GGCC'C
Cuts at: 0 144 229 1395
Size: 144 85 1166
Fragments arranged by size:
1166 144 85
AvaI C'yCGr_G
Cuts at: 0 150 1023 1203 1395
Size: 150 873 180 192
Fragments arranged by size:
873 192 180 150
BanI G'GyrC_C
Cuts at: 0 1040 1207 1395
Size: 1040 167 188
Fragments arranged by size:
1040 188 167
BanII G_rGCy'C
Cuts at: 0 144 229 654 1307 1395
Size: 144 85 425 653 88
Fragments arranged by size:
653 425 144 88 85
BbeI G_GCGC'C
Cuts at: 0 1044 1395
Size: 1044 351
BbsI GAAGACnn'nnnn_
Cuts at: 0 458 748 938 1335 1395
Size: 458 290 190 397 60
Fragments arranged by size:
458 397 290 190 60
BbvCI CC'TCA_GC
Cuts at: 0 571 1395
Size: 571 824
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 271 305 1395
Size: 271 34 1090
Fragments arranged by size:
1090 271 34
BclI T'GATC_A
Cuts at: 0 1166 1395
Size: 1166 229
BglII A'GATC_T
Cuts at: 0 266 1395
Size: 266 1129
BlpI GC'TnA_GC
Cuts at: 0 1222 1395
Size: 1222 173
Bme1580I G_kGCm'C
Cuts at: 0 144 221 229 1210 1395
Size: 144 77 8 981 185
Fragments arranged by size:
981 185 144 77 8
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 559 601 688 1395
Size: 88 471 42 87 707
Fragments arranged by size:
707 471 88 87 42
Bpu10I CC'TnA_GC
Cuts at: 0 571 1395
Size: 571 824
BsaI GGTCTCn'nnnn_
Cuts at: 0 630 1395
Size: 630 765
BsaHI Gr'CG_yC
Cuts at: 0 1041 1154 1395
Size: 1041 113 241
Fragments arranged by size:
1041 241 113
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 641 661 671 691 803 833 1395
Size: 641 20 10 20 112 30 562
Fragments arranged by size:
641 562 112 30 20 20 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 199 202 265 685 1023 1131 1395
Size: 199 3 63 420 338 108 264
Fragments arranged by size:
420 338 264 199 108 63 3
BseYI C'CCAG_C
Cuts at: 0 231 1068 1187 1395
Size: 231 837 119 208
Fragments arranged by size:
837 231 208 119
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1071 1395
Size: 1071 324
BsiHKAI G_wGCw'C
Cuts at: 0 257 415 1395
Size: 257 158 980
Fragments arranged by size:
980 257 158
BsmBI CGTCTCn'nnnn_
Cuts at: 0 391 1395
Size: 391 1004
Bsp1286I G_dGCh'C
Cuts at: 0 144 221 229 257 415 654 1210 1307
Size: 144 77 8 28 158 239 556 97
Cuts at: 1307 1395
Size: 88
Fragments arranged by size:
556 239 158 144 97 88 77 28
8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 719 1044 1070 1223 1263 1395
Size: 719 325 26 153 40 132
Fragments arranged by size:
719 325 153 132 40 26
BsrBI CCG'CTC
Cuts at: 0 174 1395
Size: 174 1221
BsrFI r'CCGG_y
Cuts at: 0 107 1395
Size: 107 1288
BstEII G'GTnAC_C
Cuts at: 0 78 1275 1395
Size: 78 1197 120
Fragments arranged by size:
1197 120 78
BstYI r'GATC_y
Cuts at: 0 158 266 788 1229 1395
Size: 158 108 522 441 166
Fragments arranged by size:
522 441 166 158 108
Bsu36I CC'TnA_GG
Cuts at: 0 758 1395
Size: 758 637
BtgI C'CryG_G
Cuts at: 0 206 344 1395
Size: 206 138 1051
Fragments arranged by size:
1051 206 138
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 564 1395
Size: 564 831
BtsI GCAGTG_nn'
Cuts at: 0 1189 1319 1395
Size: 1189 130 76
Fragments arranged by size:
1189 130 76
DraIII CAC_nnn'GTG
Cuts at: 0 345 1395
Size: 345 1050
EaeI y'GGCC_r
Cuts at: 0 563 1100 1395
Size: 563 537 295
Fragments arranged by size:
563 537 295
EarI CTCTTCn'nnn_
Cuts at: 0 961 1395
Size: 961 434
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 88 559 601 688 1395
Size: 40 48 471 42 87 707
Fragments arranged by size:
707 471 87 48 42 40
EcoNI CCTnn'n_nnAGG
Cuts at: 0 358 1395
Size: 358 1037
EcoO109I rG'GnC_Cy
Cuts at: 0 140 322 1395
Size: 140 182 1073
Fragments arranged by size:
1073 182 140
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 288 1395
Size: 288 1107
HaeII r_GCGC'y
Cuts at: 0 1044 1145 1395
Size: 1044 101 250
Fragments arranged by size:
1044 250 101
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 641 673 925 957 1395
Size: 641 32 252 32 438
Fragments arranged by size:
641 438 252 32 32
HincII GTy'rAC
Cuts at: 0 397 720 1059 1395
Size: 397 323 339 336
Fragments arranged by size:
397 339 336 323
HindIII A'AGCT_T
Cuts at: 0 460 1395
Size: 460 935
KasI G'GCGC_C
Cuts at: 0 1040 1395
Size: 1040 355
MscI TGG'CCA
Cuts at: 0 565 1395
Size: 565 830
MspA1I CmG'CkG
Cuts at: 0 66 231 1049 1068 1221 1395
Size: 66 165 818 19 153 174
Fragments arranged by size:
818 174 165 153 66 19
NaeI GCC'GGC
Cuts at: 0 109 1395
Size: 109 1286
NarI GG'CG_CC
Cuts at: 0 1041 1395
Size: 1041 354
NcoI C'CATG_G
Cuts at: 0 206 1395
Size: 206 1189
NgoMIV G'CCGG_C
Cuts at: 0 107 1395
Size: 107 1288
PasI CC'CwG_GG
Cuts at: 0 222 1395
Size: 222 1173
PfoI T'CCnGG_A
Cuts at: 0 353 1395
Size: 353 1042
PpuMI rG'GwC_Cy
Cuts at: 0 322 1395
Size: 322 1073
PspOMI G'GGCC_C
Cuts at: 0 140 225 1395
Size: 140 85 1170
Fragments arranged by size:
1170 140 85
PstI C_TGCA'G
Cuts at: 0 316 530 1067 1395
Size: 316 214 537 328
Fragments arranged by size:
537 328 316 214
PvuII CAG'CTG
Cuts at: 0 1068 1395
Size: 1068 327
SalI G'TCGA_C
Cuts at: 0 395 1395
Size: 395 1000
SanDI GG'GwC_CC
Cuts at: 0 322 1395
Size: 322 1073
SapI GCTCTTCn'nnn_
Cuts at: 0 961 1395
Size: 961 434
SfcI C'TryA_G
Cuts at: 0 312 526 1063 1395
Size: 312 214 537 332
Fragments arranged by size:
537 332 312 214
SfoI GGC'GCC
Cuts at: 0 1042 1395
Size: 1042 353
SmaI CCC'GGG
Cuts at: 0 1025 1205 1395
Size: 1025 180 190
Fragments arranged by size:
1025 190 180
StyI C'CwwG_G
Cuts at: 0 206 1395
Size: 206 1189
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 427 459 661 693 1395
Size: 427 32 202 32 702
Fragments arranged by size:
702 427 202 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 778 1153 1395
Size: 778 375 242
Fragments arranged by size:
778 375 242
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 915 1395
Size: 915 480
XmaI C'CCGG_G
Cuts at: 0 1023 1203 1395
Size: 1023 180 192
Fragments arranged by size:
1023 192 180
Enzymes that do cut:
AarI AccI AcuI AhdI AlfI AloI AlwNI ApaI
AvaI BanI BanII BbeI BbsI BbvCI BcgI BclI
BglII BlpI Bme1580I BpmI Bpu10I BsaI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI
BsrFI BstEII BstYI Bsu36I BtgI BtgZI BtsI DraIII
EaeI EarI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
HincII HindIII KasI MscI MspA1I NaeI NarI NcoI
NgoMIV PasI PfoI PpuMI PspOMI PstI PvuII SalI
SanDI SapI SfcI SfoI SmaI StyI TstI Tth111I
XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AleI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BciVI BfrBI BglI BmgBI BmrI BmtI BplI BpuEI
BsaAI BsaBI BsaWI BsiEI BsiWI BsmI BspEI BspHI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I
ClaI CspCI DraI DrdI EagI EciI EcoICRI EcoRI
EcoRV FseI FspI FspAI HpaI KpnI MfeI MluI
MmeI MslI NdeI NheI NotI NruI NsiI NspI
PacI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SbfI ScaI
SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TatI XbaI XhoI
XmnI ZraI