Restriction map for
pF1KB7701
(Linear) (Six-base) MAPSORT of: KIBB7701.seq Check: 954 from: 1 to: 1242
KIBB7701.orf
With 182 enzymes: SgfI *
October 16, 2009 16:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 615 1242
Size: 615 627
AflIII A'CryG_T
Cuts at: 0 1116 1242
Size: 1116 126
AleI CACnn'nnGTG
Cuts at: 0 118 977 1117 1242
Size: 118 859 140 125
Fragments arranged by size:
859 140 125 118
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1135 1242
Size: 1135 107
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 637 669 1242
Size: 637 32 573
Fragments arranged by size:
637 573 32
AlwNI CAG_nnn'CTG
Cuts at: 0 264 1242
Size: 264 978
ApaLI G'TGCA_C
Cuts at: 0 289 1062 1242
Size: 289 773 180
Fragments arranged by size:
773 289 180
ApoI r'AATT_y
Cuts at: 0 832 1242
Size: 832 410
AvaI C'yCGr_G
Cuts at: 0 6 597 1242
Size: 6 591 645
Fragments arranged by size:
645 591 6
BanI G'GyrC_C
Cuts at: 0 402 589 1242
Size: 402 187 653
Fragments arranged by size:
653 402 187
BbeI G_GCGC'C
Cuts at: 0 593 1242
Size: 593 649
BbvCI CC'TCA_GC
Cuts at: 0 556 1242
Size: 556 686
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 270 304 1242
Size: 270 34 938
Fragments arranged by size:
938 270 34
BglI GCCn_nnn'nGGC
Cuts at: 0 574 1242
Size: 574 668
Bme1580I G_kGCm'C
Cuts at: 0 200 293 407 1066 1156 1242
Size: 200 93 114 659 90 86
Fragments arranged by size:
659 200 114 93 90 86
BmgBI CAC'GTC
Cuts at: 0 975 1242
Size: 975 267
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 664 806 1064 1242
Size: 664 142 258 178
Fragments arranged by size:
664 258 178 142
Bpu10I CC'TnA_GC
Cuts at: 0 556 1223 1242
Size: 556 667 19
Fragments arranged by size:
667 556 19
BsaI GGTCTCn'nnnn_
Cuts at: 0 79 94 103 1242
Size: 79 15 9 1139
Fragments arranged by size:
1139 79 15 9
BsaBI GATnn'nnATC
Cuts at: 0 394 1242
Size: 394 848
BsaHI Gr'CG_yC
Cuts at: 0 590 1242
Size: 590 652
BsaWI w'CCGG_w
Cuts at: 0 1132 1242
Size: 1132 110
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 637 667 1242
Size: 637 30 575
Fragments arranged by size:
637 575 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 142 145 148 151 343 1242
Size: 142 3 3 3 192 899
Fragments arranged by size:
899 192 142 3 3 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 337 649 891 934 1001 1242
Size: 52 285 312 242 43 67 241
Fragments arranged by size:
312 285 242 241 67 52 43
BsiEI CG_ry'CG
Cuts at: 0 53 416 467 510 1242
Size: 53 363 51 43 732
Fragments arranged by size:
732 363 53 51 43
BsiHKAI G_wGCw'C
Cuts at: 0 293 1066 1242
Size: 293 773 176
Fragments arranged by size:
773 293 176
Bsp1286I G_dGCh'C
Cuts at: 0 200 293 407 1066 1156 1242
Size: 200 93 114 659 90 86
Fragments arranged by size:
659 200 114 93 90 86
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 615 1242
Size: 615 627
BsrBI CCG'CTC
Cuts at: 0 476 539 1242
Size: 476 63 703
Fragments arranged by size:
703 476 63
BsrFI r'CCGG_y
Cuts at: 0 48 202 411 415 466 568 1242
Size: 48 154 209 4 51 102 674
Fragments arranged by size:
674 209 154 102 51 48 4
BssHII G'CGCG_C
Cuts at: 0 363 1242
Size: 363 879
BssSI C'ACGA_G
Cuts at: 0 70 1242
Size: 70 1172
BstAPI GCAn_nnn'nTGC
Cuts at: 0 835 1242
Size: 835 407
BstBI TT'CG_AA
Cuts at: 0 1070 1242
Size: 1070 172
BstEII G'GTnAC_C
Cuts at: 0 267 450 660 1242
Size: 267 183 210 582
Fragments arranged by size:
582 267 210 183
BstXI CCAn_nnnn'nTGG
Cuts at: 0 96 120 825 1242
Size: 96 24 705 417
Fragments arranged by size:
705 417 96 24
BstYI r'GATC_y
Cuts at: 0 813 1242
Size: 813 429
BtgI C'CryG_G
Cuts at: 0 29 174 192 455 957 1242
Size: 29 145 18 263 502 285
Fragments arranged by size:
502 285 263 145 29 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 9 1242
Size: 9 1233
DraIII CAC_nnn'GTG
Cuts at: 0 668 1242
Size: 668 574
EaeI y'GGCC_r
Cuts at: 0 50 189 204 413 464 507 932 1242
Size: 50 139 15 209 51 43 425 310
Fragments arranged by size:
425 310 209 139 51 50 43 15
EagI C'GGCC_G
Cuts at: 0 50 413 464 507 1242
Size: 50 363 51 43 735
Fragments arranged by size:
735 363 51 50 43
EarI CTCTTCn'nnn_
Cuts at: 0 529 541 1242
Size: 529 12 701
Fragments arranged by size:
701 529 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 664 806 1064 1242
Size: 664 142 258 178
Fragments arranged by size:
664 258 178 142
EcoNI CCTnn'n_nnAGG
Cuts at: 0 785 899 1242
Size: 785 114 343
Fragments arranged by size:
785 343 114
EcoO109I rG'GnC_Cy
Cuts at: 0 1142 1242
Size: 1142 100
FspI TGC'GCA
Cuts at: 0 1111 1242
Size: 1111 131
HaeII r_GCGC'y
Cuts at: 0 593 1242
Size: 593 649
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 930 959 962 991 1242
Size: 930 29 3 29 251
Fragments arranged by size:
930 251 29 29 3
HincII GTy'rAC
Cuts at: 0 1190 1242
Size: 1190 52
HindIII A'AGCT_T
Cuts at: 0 995 1242
Size: 995 247
KasI G'GCGC_C
Cuts at: 0 589 1242
Size: 589 653
MslI CAynn'nnrTG
Cuts at: 0 94 118 400 913 977 1117 1242
Size: 94 24 282 513 64 140 125
Fragments arranged by size:
513 282 140 125 94 64 24
MspA1I CmG'CkG
Cuts at: 0 264 560 1242
Size: 264 296 682
Fragments arranged by size:
682 296 264
NaeI GCC'GGC
Cuts at: 0 50 204 413 417 468 570 1242
Size: 50 154 209 4 51 102 672
Fragments arranged by size:
672 209 154 102 51 50 4
NarI GG'CG_CC
Cuts at: 0 590 1242
Size: 590 652
NgoMIV G'CCGG_C
Cuts at: 0 48 202 411 415 466 568 1242
Size: 48 154 209 4 51 102 674
Fragments arranged by size:
674 209 154 102 51 48 4
NotI GC'GGCC_GC
Cuts at: 0 507 1242
Size: 507 735
NspI r_CATG'y
Cuts at: 0 1120 1222 1242
Size: 1120 102 20
Fragments arranged by size:
1120 102 20
PciI A'CATG_T
Cuts at: 0 1116 1242
Size: 1116 126
PfoI T'CCnGG_A
Cuts at: 0 783 1242
Size: 783 459
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 637 669 1242
Size: 637 32 573
Fragments arranged by size:
637 573 32
PstI C_TGCA'G
Cuts at: 0 260 1242
Size: 260 982
SapI GCTCTTCn'nnn_
Cuts at: 0 529 1242
Size: 529 713
SfcI C'TryA_G
Cuts at: 0 256 1242
Size: 256 986
SfoI GGC'GCC
Cuts at: 0 591 1242
Size: 591 651
SmaI CCC'GGG
Cuts at: 0 599 1242
Size: 599 643
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 648 970 1242
Size: 648 322 272
Fragments arranged by size:
648 322 272
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 945 977 1242
Size: 945 32 265
Fragments arranged by size:
945 265 32
XmaI C'CCGG_G
Cuts at: 0 597 1242
Size: 597 645
Enzymes that do cut:
AarI AflIII AleI AlfI AloI AlwNI ApaLI ApoI
AvaI BanI BbeI BbvCI BcgI BglI Bme1580I BmgBI
BpmI Bpu10I BsaI BsaBI BsaHI BsaWI BsaXI BseRI
BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
DraIII EaeI EagI EarI Eco57MI EcoNI EcoO109I FspI
HaeII Hin4I HincII HindIII KasI MslI MspA1I NaeI
NarI NgoMIV NotI NspI PciI PfoI PpiI PstI
SapI SfcI SfoI SmaI TaqII TstI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI ApaI AscI AseI AsiSI AvrII BaeI BamHI
BanII BbsI BciVI BclI BfrBI BglII BlpI BmrI
BmtI BplI BpuEI BsaAI BseYI BsiWI BsmI BsmBI
BspEI BspHI BsrDI BsrGI BstZ17I Bsu36I BtsI ClaI
CspCI DraI DrdI EciI EcoICRI EcoRI EcoRV FalI
FseI FspAI HpaI KpnI MfeI MluI MmeI MscI
NcoI NdeI NheI NruI NsiI PacI PasI PflMI
PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TatI Tth111I
XbaI XcmI XhoI XmnI ZraI