(Linear) (Six-base) MAPSORT of: KIBB7690.seq  Check: 8470  from: 1  to: 1233



KIBB7690.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:08  ..

AccI GT'mk_AC

Cuts at:      0     471    1084    1121    1233 
   Size:        471     613      37     112

  Fragments arranged by size: 

                 613     471     112      37
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     548    1233 
   Size:        548     685
 
AhdI GACnn_n'nnGTC

Cuts at:      0     645    1233 
   Size:        645     588
 
AlwNI CAG_nnn'CTG

Cuts at:      0      43     451    1233 
   Size:         43     408     782

  Fragments arranged by size: 

                 782     408      43
 
ApoI r'AATT_y

Cuts at:      0     614     772    1233 
   Size:        614     158     461

  Fragments arranged by size: 

                 614     461     158
 
AvaI C'yCGr_G

Cuts at:      0     132    1233 
   Size:        132    1101
 
BanI G'GyrC_C

Cuts at:      0     633    1233 
   Size:        633     600
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      73    1233 
   Size:         73    1160
 
BglII A'GATC_T

Cuts at:      0     315    1233 
   Size:        315     918
 
BlpI GC'TnA_GC

Cuts at:      0     932    1233 
   Size:        932     301
 
BmgBI CAC'GTC

Cuts at:      0    1082    1233 
   Size:       1082     151
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     500    1233 
   Size:        500     733
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      24      54    1071    1101    1233 
   Size:         24      30    1017      30     132

  Fragments arranged by size: 

                1017     132      30      30      24
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1003    1042    1204    1233 
   Size:       1003      39     162      29

  Fragments arranged by size: 

                1003     162      39      29
 
BseYI C'CCAG_C

Cuts at:      0      36      58     120    1233 
   Size:         36      22      62    1113

  Fragments arranged by size: 

                1113      62      36      22
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     464    1233 
   Size:        464     769
 
BsiHKAI G_wGCw'C

Cuts at:      0     139    1233 
   Size:        139    1094
 
BsmI GAATG_Cn'

Cuts at:      0     515     674    1233 
   Size:        515     159     559

  Fragments arranged by size: 

                 559     515     159
 
Bsp1286I G_dGCh'C

Cuts at:      0     139    1233 
   Size:        139    1094
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     663     678    1233 
   Size:        663      15     555

  Fragments arranged by size: 

                 663     555      15
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     451    1233 
   Size:        451     782
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1066    1233 
   Size:       1066     167
 
BstYI r'GATC_y

Cuts at:      0     315     531    1203    1233 
   Size:        315     216     672      30

  Fragments arranged by size: 

                 672     315     216      30
 
Bsu36I CC'TnA_GG

Cuts at:      0     959    1091    1233 
   Size:        959     132     142

  Fragments arranged by size: 

                 959     142     132
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1086    1121    1233 
   Size:       1086      35     112

  Fragments arranged by size: 

                1086     112      35
 
DraI TTT'AAA

Cuts at:      0     347    1233 
   Size:        347     886
 
EarI CTCTTCn'nnn_

Cuts at:      0     760    1233 
   Size:        760     473
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     548    1233 
   Size:        548     685
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     219    1233 
   Size:        219    1014
 
EcoO109I rG'GnC_Cy

Cuts at:      0     675    1233 
   Size:        675     558
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      32     748    1233 
   Size:         32     716     485

  Fragments arranged by size: 

                 716     485      32
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     631     663    1233 
   Size:        631      32     570

  Fragments arranged by size: 

                 631     570      32
 
HincII GTy'rAC

Cuts at:      0     472    1122    1233 
   Size:        472     650     111

  Fragments arranged by size: 

                 650     472     111
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     284    1233 
   Size:        284     949
 
MslI CAynn'nnrTG

Cuts at:      0    1015    1233 
   Size:       1015     218
 
MspA1I CmG'CkG

Cuts at:      0     207     859    1233 
   Size:        207     652     374

  Fragments arranged by size: 

                 652     374     207
 
NspI r_CATG'y

Cuts at:      0     309    1233 
   Size:        309     924
 
PfoI T'CCnGG_A

Cuts at:      0     776    1233 
   Size:        776     457
 
PpuMI rG'GwC_Cy

Cuts at:      0     675    1233 
   Size:        675     558
 
PvuII CAG'CTG

Cuts at:      0     859    1233 
   Size:        859     374
 
SalI G'TCGA_C

Cuts at:      0     470    1120    1233 
   Size:        470     650     113

  Fragments arranged by size: 

                 650     470     113
 
SfcI C'TryA_G

Cuts at:      0     114    1233 
   Size:        114    1119
 
SspI AAT'ATT

Cuts at:      0     343    1233 
   Size:        343     890
 
StuI AGG'CCT

Cuts at:      0     394    1233 
   Size:        394     839
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     635    1233 
   Size:        635     598
 
TatI w'GTAC_w

Cuts at:      0     374    1233 
   Size:        374     859
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      24      56    1071    1103    1233 
   Size:         24      32    1015      32     130

  Fragments arranged by size: 

                1015     130      32      32      24
 
XmnI GAAnn'nnTTC

Cuts at:      0     734    1233 
   Size:        734     499
 

 Enzymes that do cut:

     AccI     AcuI     AhdI    AlwNI     ApoI     AvaI     BanI    BciVI
    BglII     BlpI    BmgBI     BplI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspMI   BstAPI    BstXI    BstYI   Bsu36I
    CspCI     DraI     EarI  Eco57MI    EcoNI EcoO109I     FalI    Hin4I
   HincII     MmeI     MslI   MspA1I     NspI     PfoI    PpuMI    PvuII
     SalI     SfcI     SspI     StuI    TaqII     TatI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI    BanII     BbeI     BbsI    BbvCI     BcgI
     BclI    BfrBI     BglI Bme1580I     BmrI     BmtI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsiEI    BsiWI
    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI    BstBI   BstEII  BstZ17I     BtgI    BtgZI     BtsI     ClaI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     StyI     SwaI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI