Restriction map for
pF1KB7682
(Linear) (Six-base) MAPSORT of: KIBB7682.seq Check: 1784 from: 1 to: 1218
KIBB7682.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 804 931 1218
Size: 804 127 287
Fragments arranged by size:
804 287 127
AccI GT'mk_AC
Cuts at: 0 390 1172 1218
Size: 390 782 46
Fragments arranged by size:
782 390 46
Acc65I G'GTAC_C
Cuts at: 0 339 1218
Size: 339 879
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1133 1218
Size: 1133 85
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 310 1218
Size: 310 908
AlwNI CAG_nnn'CTG
Cuts at: 0 510 1218
Size: 510 708
ApaI G_GGCC'C
Cuts at: 0 157 376 427 556 622 959 1218
Size: 157 219 51 129 66 337 259
Fragments arranged by size:
337 259 219 157 129 66 51
ApaLI G'TGCA_C
Cuts at: 0 1071 1218
Size: 1071 147
BanI G'GyrC_C
Cuts at: 0 12 339 924 991 1218
Size: 12 327 585 67 227
Fragments arranged by size:
585 327 227 67 12
BanII G_rGCy'C
Cuts at: 0 157 252 376 427 556 622 660 701
Size: 157 95 124 51 129 66 38 41
Cuts at: 701 824 952 959 1218
Size: 123 128 7 259
Fragments arranged by size:
259 157 129 128 124 123 95 66
51 41 38 7
BbeI G_GCGC'C
Cuts at: 0 16 1218
Size: 16 1202
BglI GCCn_nnn'nGGC
Cuts at: 0 10 1129 1218
Size: 10 1119 89
Fragments arranged by size:
1119 89 10
BlpI GC'TnA_GC
Cuts at: 0 647 1218
Size: 647 571
Bme1580I G_kGCm'C
Cuts at: 0 157 299 376 427 556 622 904 959
Size: 157 142 77 51 129 66 282 55
Cuts at: 959 1075 1218
Size: 116 143
Fragments arranged by size:
282 157 143 142 129 116 77 66
55 51
BmrI ACTGGGnnnn_n'
Cuts at: 0 527 550 860 1218
Size: 527 23 310 358
Fragments arranged by size:
527 358 310 23
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 843 1218
Size: 843 375
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 380 664 890 1218
Size: 55 325 284 226 328
Fragments arranged by size:
328 325 284 226 55
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1132 1218
Size: 1132 86
BsaI GGTCTCn'nnnn_
Cuts at: 0 678 1218
Size: 678 540
BsaHI Gr'CG_yC
Cuts at: 0 13 1218
Size: 13 1205
BsaWI w'CCGG_w
Cuts at: 0 24 738 1218
Size: 24 714 480
Fragments arranged by size:
714 480 24
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 817 847 1150 1180 1218
Size: 817 30 303 30 38
Fragments arranged by size:
817 303 38 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 665 1218
Size: 665 553
BseYI C'CCAG_C
Cuts at: 0 73 377 487 615 703 959 1218
Size: 73 304 110 128 88 256 259
Fragments arranged by size:
304 259 256 128 110 88 73
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 1218
Size: 337 881
BsiHKAI G_wGCw'C
Cuts at: 0 1075 1218
Size: 1075 143
Bsp1286I G_dGCh'C
Cuts at: 0 157 252 299 376 427 556 622 660
Size: 157 95 47 77 51 129 66 38
Cuts at: 660 701 824 904 952 959 1075 1218
Size: 41 123 80 48 7 116 143
Fragments arranged by size:
157 143 129 123 116 95 80 77
66 51 48 47 41 38 7
BspEI T'CCGG_A
Cuts at: 0 738 1218
Size: 738 480
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 804 931 1218
Size: 804 127 287
Fragments arranged by size:
804 287 127
BsrFI r'CCGG_y
Cuts at: 0 100 1218
Size: 100 1118
BstAPI GCAn_nnn'nTGC
Cuts at: 0 546 1218
Size: 546 672
BstXI CCAn_nnnn'nTGG
Cuts at: 0 420 454 688 921 1218
Size: 420 34 234 233 297
Fragments arranged by size:
420 297 234 233 34
BstYI r'GATC_y
Cuts at: 0 1160 1218
Size: 1160 58
BtgI C'CryG_G
Cuts at: 0 200 590 1218
Size: 200 390 628
Fragments arranged by size:
628 390 200
BtsI GCAGTG_nn'
Cuts at: 0 891 1218
Size: 891 327
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 924 959 1218
Size: 924 35 259
Fragments arranged by size:
924 259 35
EaeI y'GGCC_r
Cuts at: 0 2 1218
Size: 2 1216
EarI CTCTTCn'nnn_
Cuts at: 0 109 1218
Size: 109 1109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 380 664 890 1133 1218
Size: 55 325 284 226 243 85
Fragments arranged by size:
325 284 243 226 85 55
EcoNI CCTnn'n_nnAGG
Cuts at: 0 968 1218
Size: 968 250
EcoO109I rG'GnC_Cy
Cuts at: 0 153 154 372 373 424 451 956 1218
Size: 153 1 218 1 51 27 505 262
Fragments arranged by size:
505 262 218 153 51 27 1 1
HaeII r_GCGC'y
Cuts at: 0 16 1094 1218
Size: 16 1078 124
Fragments arranged by size:
1078 124 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 675 707 1150 1182 1218
Size: 675 32 443 32 36
Fragments arranged by size:
675 443 36 32 32
HincII GTy'rAC
Cuts at: 0 391 1218
Size: 391 827
HindIII A'AGCT_T
Cuts at: 0 1115 1218
Size: 1115 103
KasI G'GCGC_C
Cuts at: 0 12 1218
Size: 12 1206
KpnI G_GTAC'C
Cuts at: 0 343 1218
Size: 343 875
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 248 979 1218
Size: 248 731 239
Fragments arranged by size:
731 248 239
MslI CAynn'nnrTG
Cuts at: 0 1078 1218
Size: 1078 140
MspA1I CmG'CkG
Cuts at: 0 1197 1218
Size: 1197 21
NarI GG'CG_CC
Cuts at: 0 13 1218
Size: 13 1205
NcoI C'CATG_G
Cuts at: 0 200 590 1218
Size: 200 390 628
Fragments arranged by size:
628 390 200
NspI r_CATG'y
Cuts at: 0 441 1218
Size: 441 777
PasI CC'CwG_GG
Cuts at: 0 447 1218
Size: 447 771
PflMI CCAn_nnn'nTGG
Cuts at: 0 283 510 1218
Size: 283 227 708
Fragments arranged by size:
708 283 227
PpuMI rG'GwC_Cy
Cuts at: 0 451 1218
Size: 451 767
PspOMI G'GGCC_C
Cuts at: 0 153 372 423 552 618 955 1218
Size: 153 219 51 129 66 337 263
Fragments arranged by size:
337 263 219 153 129 66 51
PvuII CAG'CTG
Cuts at: 0 1197 1218
Size: 1197 21
SalI G'TCGA_C
Cuts at: 0 389 1218
Size: 389 829
SexAI A'CCwGG_T
Cuts at: 0 212 687 1218
Size: 212 475 531
Fragments arranged by size:
531 475 212
SfcI C'TryA_G
Cuts at: 0 351 1218
Size: 351 867
SfoI GGC'GCC
Cuts at: 0 14 1218
Size: 14 1204
SmlI C'TyrA_G
Cuts at: 0 1147 1218
Size: 1147 71
StuI AGG'CCT
Cuts at: 0 1042 1218
Size: 1042 176
StyI C'CwwG_G
Cuts at: 0 200 282 590 1154 1218
Size: 200 82 308 564 64
Fragments arranged by size:
564 308 200 82 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 600 1218
Size: 600 618
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 389 421 817 849 1218
Size: 389 32 396 32 369
Fragments arranged by size:
396 389 369 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 689 1218
Size: 689 529
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 612 1218
Size: 612 606
XmnI GAAnn'nnTTC
Cuts at: 0 1057 1218
Size: 1057 161
Enzymes that do cut:
AarI AccI Acc65I AcuI AlfI AlwNI ApaI ApaLI
BanI BanII BbeI BglI BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspEI BspMI BsrFI BstAPI BstXI
BstYI BtgI BtsI CspCI EaeI EarI Eco57MI EcoNI
EcoO109I HaeII Hin4I HincII HindIII KasI KpnI MmeI
MslI MspA1I NarI NcoI NspI PasI PflMI PpuMI
PspOMI PvuII SalI SexAI SfcI SfoI SmlI StuI
StyI TaqII TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AhdI AleI
AloI ApoI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BglII
BmgBI BmtI Bpu10I BsaAI BsaBI BsiEI BsiWI BsmI
BsmBI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI
EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HpaI MfeI MluI MscI NaeI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI
RsrII SacI SacII SanDI SapI SbfI ScaI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TatI XbaI XhoI XmaI ZraI