Restriction map for
pF1KB7680
(Linear) (Six-base) MAPSORT of: KIBB7680.seq Check: 8328 from: 1 to: 1218
KIBB7680.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 1218
Size: 184 1034
AlwNI CAG_nnn'CTG
Cuts at: 0 203 592 1218
Size: 203 389 626
Fragments arranged by size:
626 389 203
ApaI G_GGCC'C
Cuts at: 0 459 736 756 1218
Size: 459 277 20 462
Fragments arranged by size:
462 459 277 20
ApoI r'AATT_y
Cuts at: 0 1159 1218
Size: 1159 59
AvaI C'yCGr_G
Cuts at: 0 526 913 1218
Size: 526 387 305
Fragments arranged by size:
526 387 305
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 469 502 1218
Size: 469 33 716
Fragments arranged by size:
716 469 33
BamHI G'GATC_C
Cuts at: 0 1209 1218
Size: 1209 9
BanI G'GyrC_C
Cuts at: 0 676 964 1012 1218
Size: 676 288 48 206
Fragments arranged by size:
676 288 206 48
BanII G_rGCy'C
Cuts at: 0 415 459 527 562 736 756 791 1218
Size: 415 44 68 35 174 20 35 427
Fragments arranged by size:
427 415 174 68 44 35 35 20
BbeI G_GCGC'C
Cuts at: 0 968 1218
Size: 968 250
BbsI GAAGACnn'nnnn_
Cuts at: 0 695 859 1218
Size: 695 164 359
Fragments arranged by size:
695 359 164
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 894 928 1218
Size: 894 34 290
Fragments arranged by size:
894 290 34
BglI GCCn_nnn'nGGC
Cuts at: 0 951 1061 1218
Size: 951 110 157
Fragments arranged by size:
951 157 110
BglII A'GATC_T
Cuts at: 0 326 1218
Size: 326 892
Bme1580I G_kGCm'C
Cuts at: 0 145 459 736 756 1218
Size: 145 314 277 20 462
Fragments arranged by size:
462 314 277 145 20
BmrI ACTGGGnnnn_n'
Cuts at: 0 869 1218
Size: 869 349
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 399 977 1078 1121 1162 1204 1218
Size: 399 578 101 43 41 42 14
Fragments arranged by size:
578 399 101 43 42 41 14
Bpu10I CC'TnA_GC
Cuts at: 0 382 1218
Size: 382 836
BsaHI Gr'CG_yC
Cuts at: 0 7 965 1064 1218
Size: 7 958 99 154
Fragments arranged by size:
958 154 99 7
BsaWI w'CCGG_w
Cuts at: 0 65 976 1218
Size: 65 911 242
Fragments arranged by size:
911 242 65
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 539 569 868 898 1218
Size: 539 30 299 30 320
Fragments arranged by size:
539 320 299 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 439 1034 1051 1218
Size: 439 595 17 167
Fragments arranged by size:
595 439 167 17
BseYI C'CCAG_C
Cuts at: 0 458 729 757 1051 1218
Size: 458 271 28 294 167
Fragments arranged by size:
458 294 271 167 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 376 1218
Size: 46 330 842
Fragments arranged by size:
842 330 46
BsiEI CG_ry'CG
Cuts at: 0 475 1218
Size: 475 743
BsiHKAI G_wGCw'C
Cuts at: 0 415 562 791 1218
Size: 415 147 229 427
Fragments arranged by size:
427 415 229 147
BsmI GAATG_Cn'
Cuts at: 0 21 30 126 870 1218
Size: 21 9 96 744 348
Fragments arranged by size:
744 348 96 21 9
Bsp1286I G_dGCh'C
Cuts at: 0 145 415 459 527 562 736 756 791
Size: 145 270 44 68 35 174 20 35
Cuts at: 791 1218
Size: 427
Fragments arranged by size:
427 270 174 145 68 44 35 35
20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 265 1218
Size: 265 953
BsrBI CCG'CTC
Cuts at: 0 236 918 1218
Size: 236 682 300
Fragments arranged by size:
682 300 236
BsrDI GCAATG_nn'
Cuts at: 0 905 1218
Size: 905 313
BsrFI r'CCGG_y
Cuts at: 0 9 679 1218
Size: 9 670 539
Fragments arranged by size:
670 539 9
BssSI C'ACGA_G
Cuts at: 0 790 814 1218
Size: 790 24 404
Fragments arranged by size:
790 404 24
BstAPI GCAn_nnn'nTGC
Cuts at: 0 447 1218
Size: 447 771
BstEII G'GTnAC_C
Cuts at: 0 697 1218
Size: 697 521
BstXI CCAn_nnnn'nTGG
Cuts at: 0 894 1218
Size: 894 324
BstYI r'GATC_y
Cuts at: 0 326 1209 1218
Size: 326 883 9
Fragments arranged by size:
883 326 9
Bsu36I CC'TnA_GG
Cuts at: 0 1034 1218
Size: 1034 184
BtgI C'CryG_G
Cuts at: 0 609 1218
Size: 609 609
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1077 1218
Size: 1077 141
BtsI GCAGTG_nn'
Cuts at: 0 121 639 1218
Size: 121 518 579
Fragments arranged by size:
579 518 121
DrdI GACnn_nn'nnGTC
Cuts at: 0 217 1218
Size: 217 1001
EaeI y'GGCC_r
Cuts at: 0 292 472 1177 1218
Size: 292 180 705 41
Fragments arranged by size:
705 292 180 41
EagI C'GGCC_G
Cuts at: 0 472 1218
Size: 472 746
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 367 558 1218
Size: 367 191 660
Fragments arranged by size:
660 367 191
EcoICRI GAG'CTC
Cuts at: 0 413 560 789 1218
Size: 413 147 229 429
Fragments arranged by size:
429 413 229 147
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 399 977 1078 1121 1162 1204 1218
Size: 184 215 578 101 43 41 42 14
Fragments arranged by size:
578 215 184 101 43 42 41 14
EcoO109I rG'GnC_Cy
Cuts at: 0 455 589 733 752 753 952 982 1218
Size: 455 134 144 19 1 199 30 236
Fragments arranged by size:
455 236 199 144 134 30 19 1
FspI TGC'GCA
Cuts at: 0 904 1218
Size: 904 314
HaeII r_GCGC'y
Cuts at: 0 968 1218
Size: 968 250
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 359 391 544 576 868 900 1218
Size: 359 32 153 32 292 32 318
Fragments arranged by size:
359 318 292 153 32 32 32
KasI G'GCGC_C
Cuts at: 0 964 1218
Size: 964 254
MslI CAynn'nnrTG
Cuts at: 0 502 1218
Size: 502 716
NaeI GCC'GGC
Cuts at: 0 11 1218
Size: 11 1207
NarI GG'CG_CC
Cuts at: 0 965 1218
Size: 965 253
NgoMIV G'CCGG_C
Cuts at: 0 9 1218
Size: 9 1209
NspI r_CATG'y
Cuts at: 0 162 441 1218
Size: 162 279 777
Fragments arranged by size:
777 279 162
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 778 810 1218
Size: 778 32 408
Fragments arranged by size:
778 408 32
PpuMI rG'GwC_Cy
Cuts at: 0 982 1218
Size: 982 236
PspOMI G'GGCC_C
Cuts at: 0 455 732 752 1218
Size: 455 277 20 466
Fragments arranged by size:
466 455 277 20
PspXI vC'TCGA_Gb
Cuts at: 0 913 1218
Size: 913 305
SacI G_AGCT'C
Cuts at: 0 415 562 791 1218
Size: 415 147 229 427
Fragments arranged by size:
427 415 229 147
ScaI AGT'ACT
Cuts at: 0 892 1218
Size: 892 326
SfoI GGC'GCC
Cuts at: 0 966 1218
Size: 966 252
SgrAI Cr'CCGG_yG
Cuts at: 0 9 1218
Size: 9 1209
SmaI CCC'GGG
Cuts at: 0 528 1218
Size: 528 690
SmlI C'TyrA_G
Cuts at: 0 913 1218
Size: 913 305
SphI G_CATG'C
Cuts at: 0 441 1218
Size: 441 777
StyI C'CwwG_G
Cuts at: 0 182 1205 1218
Size: 182 1023 13
Fragments arranged by size:
1023 182 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 107 380 658 980 1218
Size: 107 273 278 322 238
Fragments arranged by size:
322 278 273 238 107
TatI w'GTAC_w
Cuts at: 0 890 1079 1218
Size: 890 189 139
Fragments arranged by size:
890 189 139
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 539 571 1194 1218
Size: 539 32 623 24
Fragments arranged by size:
623 539 32 24
XhoI C'TCGA_G
Cuts at: 0 913 1218
Size: 913 305
XmaI C'CCGG_G
Cuts at: 0 526 1218
Size: 526 692
Enzymes that do cut:
AcuI AlwNI ApaI ApoI AvaI BaeI BamHI BanI
BanII BbeI BbsI BcgI BglI BglII Bme1580I BmrI
BpmI Bpu10I BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DrdI EaeI EagI EciI EcoICRI Eco57MI EcoO109I
FspI HaeII Hin4I KasI MslI NaeI NarI NgoMIV
NspI PpiI PpuMI PspOMI PspXI SacI ScaI SfoI
SgrAI SmaI SmlI SphI StyI TaqII TatI TstI
XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI AscI AseI
AsiSI AvrII BbvCI BciVI BclI BfrBI BlpI BmgBI
BmtI BplI BpuEI BsaI BsaAI BsaBI BsiWI BsmBI
BspEI BspHI BsrGI BssHII BstBI BstZ17I ClaI CspCI
DraI DraIII EarI EcoNI EcoRI EcoRV FalI FseI
FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MspA1I NcoI NdeI NheI NotI NruI NsiI
PacI PasI PciI PflMI PfoI PmeI PmlI PshAI
PsiI PsrI PstI PvuI PvuII RsrII SacII SalI
SanDI SapI SbfI SexAI SfcI SfiI SgfI SnaBI
SpeI SrfI SspI StuI SwaI Tth111I XbaI XcmI
XmnI ZraI