Restriction map for
pF1KB7671
(Linear) (Six-base) MAPSORT of: KIBB7671.seq Check: 291 from: 1 to: 1014
KIBB7671.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 513 570 662 1014
Size: 513 57 92 352
Fragments arranged by size:
513 352 92 57
AfeI AGC'GCT
Cuts at: 0 343 496 1014
Size: 343 153 518
Fragments arranged by size:
518 343 153
AflIII A'CryG_T
Cuts at: 0 545 1014
Size: 545 469
AleI CACnn'nnGTG
Cuts at: 0 814 1014
Size: 814 200
AlwNI CAG_nnn'CTG
Cuts at: 0 357 483 654 669 861 1014
Size: 357 126 171 15 192 153
Fragments arranged by size:
357 192 171 153 126 15
ApaI G_GGCC'C
Cuts at: 0 763 1014
Size: 763 251
AvaI C'yCGr_G
Cuts at: 0 112 1014
Size: 112 902
BanI G'GyrC_C
Cuts at: 0 15 30 841 1014
Size: 15 15 811 173
Fragments arranged by size:
811 173 15 15
BanII G_rGCy'C
Cuts at: 0 83 763 1014
Size: 83 680 251
Fragments arranged by size:
680 251 83
BbsI GAAGACnn'nnnn_
Cuts at: 0 313 1014
Size: 313 701
BlpI GC'TnA_GC
Cuts at: 0 247 358 1014
Size: 247 111 656
Fragments arranged by size:
656 247 111
Bme1580I G_kGCm'C
Cuts at: 0 20 35 763 844 1014
Size: 20 15 728 81 170
Fragments arranged by size:
728 170 81 20 15
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 262 1014
Size: 262 752
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 316 535 556 655 883 1014
Size: 250 66 219 21 99 228 131
Fragments arranged by size:
250 228 219 131 99 66 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 322 547 1014
Size: 196 126 225 467
Fragments arranged by size:
467 225 196 126
BsaHI Gr'CG_yC
Cuts at: 0 965 1014
Size: 965 49
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 274 304 922 952 976 1006 1014
Size: 274 30 618 30 24 30 8
Fragments arranged by size:
618 274 30 30 30 24 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 532 713 716 756 880 883 1000 1014
Size: 532 181 3 40 124 3 117 14
Fragments arranged by size:
532 181 124 117 40 14 3 3
BseYI C'CCAG_C
Cuts at: 0 662 1014
Size: 662 352
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 838 1014
Size: 604 234 176
Fragments arranged by size:
604 234 176
BsmBI CGTCTCn'nnnn_
Cuts at: 0 168 1014
Size: 168 846
Bsp1286I G_dGCh'C
Cuts at: 0 20 35 83 763 844 1014
Size: 20 15 48 680 81 170
Fragments arranged by size:
680 170 81 48 20 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 458 513 570 662 1014
Size: 458 55 57 92 352
Fragments arranged by size:
458 352 92 57 55
BsrBI CCG'CTC
Cuts at: 0 135 171 362 639 1014
Size: 135 36 191 277 375
Fragments arranged by size:
375 277 191 135 36
BstAPI GCAn_nnn'nTGC
Cuts at: 0 582 654 1014
Size: 582 72 360
Fragments arranged by size:
582 360 72
BtgI C'CryG_G
Cuts at: 0 698 755 968 1014
Size: 698 57 213 46
Fragments arranged by size:
698 213 57 46
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 153 457 1014
Size: 153 304 557
Fragments arranged by size:
557 304 153
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 316 535 556 655 883 1014
Size: 250 66 219 21 99 228 131
Fragments arranged by size:
250 228 219 131 99 66 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 298 1014
Size: 298 716
EcoO109I rG'GnC_Cy
Cuts at: 0 447 760 918 1014
Size: 447 313 158 96
Fragments arranged by size:
447 313 158 96
FspI TGC'GCA
Cuts at: 0 505 575 1014
Size: 505 70 439
Fragments arranged by size:
505 439 70
FspAI rTGC'GCAy
Cuts at: 0 505 1014
Size: 505 509
HaeII r_GCGC'y
Cuts at: 0 345 498 1014
Size: 345 153 516
Fragments arranged by size:
516 345 153
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 872 904 922 954 976 1008 1014
Size: 872 32 18 32 22 32 6
Fragments arranged by size:
872 32 32 32 22 18 6
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 965 1014
Size: 965 49
MslI CAynn'nnrTG
Cuts at: 0 743 814 1014
Size: 743 71 200
Fragments arranged by size:
743 200 71
MspA1I CmG'CkG
Cuts at: 0 123 483 588 876 908 1014
Size: 123 360 105 288 32 106
Fragments arranged by size:
360 288 123 106 105 32
NcoI C'CATG_G
Cuts at: 0 755 968 1014
Size: 755 213 46
Fragments arranged by size:
755 213 46
NspI r_CATG'y
Cuts at: 0 183 549 1014
Size: 183 366 465
Fragments arranged by size:
465 366 183
PciI A'CATG_T
Cuts at: 0 545 1014
Size: 545 469
PfoI T'CCnGG_A
Cuts at: 0 350 1014
Size: 350 664
PpuMI rG'GwC_Cy
Cuts at: 0 447 1014
Size: 447 567
PspOMI G'GGCC_C
Cuts at: 0 759 1014
Size: 759 255
PstI C_TGCA'G
Cuts at: 0 593 1014
Size: 593 421
PvuII CAG'CTG
Cuts at: 0 483 588 908 1014
Size: 483 105 320 106
Fragments arranged by size:
483 320 106 105
SfcI C'TryA_G
Cuts at: 0 84 589 1014
Size: 84 505 425
Fragments arranged by size:
505 425 84
SmlI C'TyrA_G
Cuts at: 0 211 337 562 1014
Size: 211 126 225 452
Fragments arranged by size:
452 225 211 126
SphI G_CATG'C
Cuts at: 0 183 1014
Size: 183 831
StuI AGG'CCT
Cuts at: 0 952 1014
Size: 952 62
StyI C'CwwG_G
Cuts at: 0 755 968 1014
Size: 755 213 46
Fragments arranged by size:
755 213 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 703 759 859 1014
Size: 703 56 100 155
Fragments arranged by size:
703 155 100 56
TatI w'GTAC_w
Cuts at: 0 281 1014
Size: 281 733
Enzymes that do cut:
AarI AfeI AflIII AleI AlwNI ApaI AvaI BanI
BanII BbsI BlpI Bme1580I BplI BpmI BpuEI BsaHI
BsaXI BseRI BseYI BsgI BsmBI Bsp1286I BspMI BsrBI
BstAPI BtgI EciI Eco57MI EcoNI EcoO109I FspI FspAI
HaeII Hin4I MmeI MslI MspA1I NcoI NspI PciI
PfoI PpuMI PspOMI PstI PvuII SfcI SmlI SphI
StuI StyI TaqII TatI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AgeI AhdI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BmgBI BmrI BmtI Bpu10I BsaI BsaAI
BsaBI BsaWI BsiEI BsiHKAI BsiWI BsmI BspEI BspHI
BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EcoICRI EcoRI EcoRV
FalI FseI HincII HindIII HpaI KasI KpnI MfeI
MluI MscI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PflMI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI