Restriction map for
pF1KB7670
(Linear) (Six-base) MAPSORT of: KIBB7670.seq Check: 8878 from: 1 to: 1185
KIBB7670.orf
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 350 489 876 1088 1185
Size: 350 139 387 212 97
Fragments arranged by size:
387 350 212 139 97
ApaI G_GGCC'C
Cuts at: 0 116 1185
Size: 116 1069
ApoI r'AATT_y
Cuts at: 0 463 1185
Size: 463 722
AvaI C'yCGr_G
Cuts at: 0 19 109 1185
Size: 19 90 1076
Fragments arranged by size:
1076 90 19
BanI G'GyrC_C
Cuts at: 0 839 1185
Size: 839 346
BanII G_rGCy'C
Cuts at: 0 116 142 541 976 1117 1185
Size: 116 26 399 435 141 68
Fragments arranged by size:
435 399 141 116 68 26
BbvCI CC'TCA_GC
Cuts at: 0 1081 1185
Size: 1081 104
BglI GCCn_nnn'nGGC
Cuts at: 0 703 980 1185
Size: 703 277 205
Fragments arranged by size:
703 277 205
BlpI GC'TnA_GC
Cuts at: 0 526 1185
Size: 526 659
Bme1580I G_kGCm'C
Cuts at: 0 116 1185
Size: 116 1069
BmgBI CAC'GTC
Cuts at: 0 1110 1185
Size: 1110 75
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 570 1185
Size: 570 615
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 415 1185
Size: 49 366 770
Fragments arranged by size:
770 366 49
Bpu10I CC'TnA_GC
Cuts at: 0 1081 1185
Size: 1081 104
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 1185
Size: 457 728
BsaI GGTCTCn'nnnn_
Cuts at: 0 723 1185
Size: 723 462
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 388 418 877 907 1099 1129 1165 1185
Size: 388 30 459 30 192 30 36 20
Fragments arranged by size:
459 388 192 36 30 30 30 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 106 247 1092 1185
Size: 106 141 845 93
Fragments arranged by size:
845 141 106 93
BseYI C'CCAG_C
Cuts at: 0 135 869 876 1185
Size: 135 734 7 309
Fragments arranged by size:
734 309 135 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 363 1185
Size: 363 822
BsiEI CG_ry'CG
Cuts at: 0 195 1149 1185
Size: 195 954 36
Fragments arranged by size:
954 195 36
BsiHKAI G_wGCw'C
Cuts at: 0 98 473 1185
Size: 98 375 712
Fragments arranged by size:
712 375 98
BsmBI CGTCTCn'nnnn_
Cuts at: 0 757 1185
Size: 757 428
Bsp1286I G_dGCh'C
Cuts at: 0 98 116 142 473 541 976 1117 1185
Size: 98 18 26 331 68 435 141 68
Fragments arranged by size:
435 331 141 98 68 68 26 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 530 1185
Size: 530 655
BsrBI CCG'CTC
Cuts at: 0 441 1185
Size: 441 744
BsrFI r'CCGG_y
Cuts at: 0 591 1148 1185
Size: 591 557 37
Fragments arranged by size:
591 557 37
BssSI C'ACGA_G
Cuts at: 0 229 1185
Size: 229 956
BstAPI GCAn_nnn'nTGC
Cuts at: 0 102 350 1185
Size: 102 248 835
Fragments arranged by size:
835 248 102
BstXI CCAn_nnnn'nTGG
Cuts at: 0 72 903 1185
Size: 72 831 282
Fragments arranged by size:
831 282 72
BtgI C'CryG_G
Cuts at: 0 646 962 1185
Size: 646 316 223
Fragments arranged by size:
646 316 223
BtsI GCAGTG_nn'
Cuts at: 0 250 1185
Size: 250 935
EaeI y'GGCC_r
Cuts at: 0 335 593 959 1185
Size: 335 258 366 226
Fragments arranged by size:
366 335 258 226
EarI CTCTTCn'nnn_
Cuts at: 0 334 556 687 1185
Size: 334 222 131 498
Fragments arranged by size:
498 334 222 131
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 415 1185
Size: 49 366 770
Fragments arranged by size:
770 366 49
EcoO109I rG'GnC_Cy
Cuts at: 0 16 519 642 888 1005 1185
Size: 16 503 123 246 117 180
Fragments arranged by size:
503 246 180 123 117 16
EcoRI G'AATT_C
Cuts at: 0 463 1185
Size: 463 722
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 726 1185
Size: 726 459
HaeII r_GCGC'y
Cuts at: 0 394 1185
Size: 394 791
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 706 738 877 909 1099 1131 1185
Size: 706 32 139 32 190 32 54
Fragments arranged by size:
706 190 139 54 32 32 32
HincII GTy'rAC
Cuts at: 0 213 1185
Size: 213 972
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 167 215 575 1185
Size: 53 114 48 360 610
Fragments arranged by size:
610 360 114 53 48
MscI TGG'CCA
Cuts at: 0 961 1185
Size: 961 224
MslI CAynn'nnrTG
Cuts at: 0 85 1185
Size: 85 1100
MspA1I CmG'CkG
Cuts at: 0 180 278 347 486 626 648 760 873
Size: 180 98 69 139 140 22 112 113
Cuts at: 873 933 1185
Size: 60 252
Fragments arranged by size:
252 180 140 139 113 112 98 69
60 22
NaeI GCC'GGC
Cuts at: 0 593 1185
Size: 593 592
NcoI C'CATG_G
Cuts at: 0 962 1185
Size: 962 223
NgoMIV G'CCGG_C
Cuts at: 0 591 1185
Size: 591 594
PflMI CCAn_nnn'nTGG
Cuts at: 0 876 1185
Size: 876 309
PfoI T'CCnGG_A
Cuts at: 0 287 1185
Size: 287 898
PpuMI rG'GwC_Cy
Cuts at: 0 16 519 642 888 1185
Size: 16 503 123 246 297
Fragments arranged by size:
503 297 246 123 16
PspOMI G'GGCC_C
Cuts at: 0 112 1185
Size: 112 1073
PstI C_TGCA'G
Cuts at: 0 1124 1185
Size: 1124 61
PvuII CAG'CTG
Cuts at: 0 278 486 760 1185
Size: 278 208 274 425
Fragments arranged by size:
425 278 274 208
SacII CC_GC'GG
Cuts at: 0 649 1185
Size: 649 536
SapI GCTCTTCn'nnn_
Cuts at: 0 334 556 687 1185
Size: 334 222 131 498
Fragments arranged by size:
498 334 222 131
SbfI CC_TGCA'GG
Cuts at: 0 1124 1185
Size: 1124 61
SfcI C'TryA_G
Cuts at: 0 1120 1185
Size: 1120 65
SmaI CCC'GGG
Cuts at: 0 111 1185
Size: 111 1074
SmlI C'TyrA_G
Cuts at: 0 472 1185
Size: 472 713
StuI AGG'CCT
Cuts at: 0 706 1185
Size: 706 479
StyI C'CwwG_G
Cuts at: 0 596 962 1185
Size: 596 366 223
Fragments arranged by size:
596 366 223
TatI w'GTAC_w
Cuts at: 0 302 1185
Size: 302 883
XmaI C'CCGG_G
Cuts at: 0 109 1185
Size: 109 1076
Enzymes that do cut:
AlwNI ApaI ApoI AvaI BanI BanII BbvCI BglI
BlpI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspMI BsrBI BsrFI BssSI BstAPI BstXI BtgI BtsI
EaeI EarI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I
HincII MmeI MscI MslI MspA1I NaeI NcoI NgoMIV
PflMI PfoI PpuMI PspOMI PstI PvuII SacII SapI
SbfI SfcI SmaI SmlI StuI StyI TatI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbsI BcgI
BciVI BclI BfrBI BglII BmrI BmtI BsaAI BsaBI
BsaHI BsaWI BsiWI BsmI BspEI BspHI BsrDI BsrGI
BssHII BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI
EcoRV FseI FspI FspAI HindIII HpaI KasI KpnI
MfeI MluI NarI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI TaqII TstI Tth111I XbaI
XcmI XhoI XmnI ZraI