Restriction map for
pF1KB7666
(Linear) (Six-base) MAPSORT of: KIBB7666.seq Check: 5012 from: 1 to: 1113
KIBB7666.orf
With 182 enzymes: SgfI *
January 9, 2015 15:32 ..
AflIII A'CryG_T
Cuts at: 0 374 1113
Size: 374 739
AhdI GACnn_n'nnGTC
Cuts at: 0 159 1113
Size: 159 954
AlwNI CAG_nnn'CTG
Cuts at: 0 571 1113
Size: 571 542
AvaI C'yCGr_G
Cuts at: 0 324 405 1113
Size: 324 81 708
Fragments arranged by size:
708 324 81
BanII G_rGCy'C
Cuts at: 0 245 1113
Size: 245 868
BbsI GAAGACnn'nnnn_
Cuts at: 0 155 1113
Size: 155 958
BbvCI CC'TCA_GC
Cuts at: 0 767 1113
Size: 767 346
BglII A'GATC_T
Cuts at: 0 107 1113
Size: 107 1006
Bme1580I G_kGCm'C
Cuts at: 0 428 1113
Size: 428 685
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 482 548 1113
Size: 482 66 565
Fragments arranged by size:
565 482 66
Bpu10I CC'TnA_GC
Cuts at: 0 767 1113
Size: 767 346
BsaAI yAC'GTr
Cuts at: 0 759 1113
Size: 759 354
BsaWI w'CCGG_w
Cuts at: 0 13 1113
Size: 13 1100
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 32 1113
Size: 32 1081
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 454 914 1113
Size: 454 460 199
Fragments arranged by size:
460 454 199
BsiHKAI G_wGCw'C
Cuts at: 0 491 1113
Size: 491 622
BsmI GAATG_Cn'
Cuts at: 0 831 1113
Size: 831 282
Bsp1286I G_dGCh'C
Cuts at: 0 245 428 491 1113
Size: 245 183 63 622
Fragments arranged by size:
622 245 183 63
BspEI T'CCGG_A
Cuts at: 0 13 1113
Size: 13 1100
BssSI C'ACGA_G
Cuts at: 0 208 1113
Size: 208 905
BstXI CCAn_nnnn'nTGG
Cuts at: 0 682 913 1113
Size: 682 231 200
Fragments arranged by size:
682 231 200
BstYI r'GATC_y
Cuts at: 0 107 385 1113
Size: 107 278 728
Fragments arranged by size:
728 278 107
Bsu36I CC'TnA_GG
Cuts at: 0 713 1113
Size: 713 400
BtgI C'CryG_G
Cuts at: 0 56 428 1113
Size: 56 372 685
Fragments arranged by size:
685 372 56
DraIII CAC_nnn'GTG
Cuts at: 0 213 1113
Size: 213 900
EaeI y'GGCC_r
Cuts at: 0 194 215 1113
Size: 194 21 898
Fragments arranged by size:
898 194 21
EarI CTCTTCn'nnn_
Cuts at: 0 128 938 1039 1045 1113
Size: 128 810 101 6 68
Fragments arranged by size:
810 128 101 68 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 482 548 1113
Size: 482 66 565
Fragments arranged by size:
565 482 66
EcoO109I rG'GnC_Cy
Cuts at: 0 39 313 1113
Size: 39 274 800
Fragments arranged by size:
800 274 39
EcoRV GAT'ATC
Cuts at: 0 576 1113
Size: 576 537
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 183 1113
Size: 183 930
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 111 143 145 177 520 552 645 677
Size: 111 32 2 32 343 32 93 32
Cuts at: 677 848 880 1113
Size: 171 32 233
Fragments arranged by size:
343 233 171 111 93 32 32 32
32 32 2
HincII GTy'rAC
Cuts at: 0 534 1113
Size: 534 579
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 591 1113
Size: 591 522
MscI TGG'CCA
Cuts at: 0 217 1113
Size: 217 896
MslI CAynn'nnrTG
Cuts at: 0 272 290 346 1113
Size: 272 18 56 767
Fragments arranged by size:
767 272 56 18
MspA1I CmG'CkG
Cuts at: 0 1102 1113
Size: 1102 11
NdeI CA'TA_TG
Cuts at: 0 650 992 1113
Size: 650 342 121
Fragments arranged by size:
650 342 121
NspI r_CATG'y
Cuts at: 0 378 552 931 1113
Size: 378 174 379 182
Fragments arranged by size:
379 378 182 174
PciI A'CATG_T
Cuts at: 0 374 1113
Size: 374 739
PflMI CCAn_nnn'nTGG
Cuts at: 0 631 1113
Size: 631 482
PfoI T'CCnGG_A
Cuts at: 0 498 1113
Size: 498 615
PmlI CAC'GTG
Cuts at: 0 759 1113
Size: 759 354
PpuMI rG'GwC_Cy
Cuts at: 0 39 313 1113
Size: 39 274 800
Fragments arranged by size:
800 274 39
PspXI vC'TCGA_Gb
Cuts at: 0 324 1113
Size: 324 789
PvuII CAG'CTG
Cuts at: 0 1102 1113
Size: 1102 11
SanDI GG'GwC_CC
Cuts at: 0 39 313 1113
Size: 39 274 800
Fragments arranged by size:
800 274 39
SapI GCTCTTCn'nnn_
Cuts at: 0 938 1113
Size: 938 175
SfcI C'TryA_G
Cuts at: 0 1063 1113
Size: 1063 50
SmlI C'TyrA_G
Cuts at: 0 324 1113
Size: 324 789
SphI G_CATG'C
Cuts at: 0 931 1113
Size: 931 182
SspI AAT'ATT
Cuts at: 0 958 1113
Size: 958 155
StuI AGG'CCT
Cuts at: 0 299 1113
Size: 299 814
StyI C'CwwG_G
Cuts at: 0 167 1113
Size: 167 946
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 414 1113
Size: 414 699
TatI w'GTAC_w
Cuts at: 0 877 1113
Size: 877 236
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 197 229 1113
Size: 197 32 884
Fragments arranged by size:
884 197 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 632 1113
Size: 632 481
XhoI C'TCGA_G
Cuts at: 0 324 1113
Size: 324 789
Enzymes that do cut:
AflIII AhdI AlwNI AvaI BanII BbsI BbvCI BglII
Bme1580I BpmI Bpu10I BsaAI BsaWI BseRI BsgI BsiHKAI
BsmI Bsp1286I BspEI BssSI BstXI BstYI Bsu36I BtgI
DraIII EaeI EarI Eco57MI EcoO109I EcoRV FalI Hin4I
HincII MmeI MscI MslI MspA1I NdeI NspI PciI
PflMI PfoI PmlI PpuMI PspXI PvuII SanDI SapI
SfcI SmlI SphI SspI StuI StyI TaqII TatI
TstI XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BcgI
BciVI BclI BfrBI BglI BlpI BmgBI BmrI BmtI
BplI BpuEI BsaI BsaBI BsaHI BsaXI BseYI BsiEI
BsiWI BsmBI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BstAPI BstBI BstEII BstZ17I BtgZI BtsI ClaI
CspCI DraI DrdI EagI EciI EcoICRI EcoNI EcoRI
FseI FspI FspAI HaeII HindIII HpaI KasI KpnI
MfeI MluI NaeI NarI NcoI NgoMIV NheI NotI
NruI NsiI PacI PasI PmeI PpiI PshAI PsiI
PspOMI PsrI PstI PvuI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SwaI Tth111I XbaI XmaI XmnI
ZraI