(Linear) (Six-base) MAPSORT of: KIBB7663.seq  Check: 2089  from: 1  to: 1008



KIBB7663.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

AccI GT'mk_AC

Cuts at:      0     132     871    1008 
   Size:        132     739     137

  Fragments arranged by size: 

                 739     137     132
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     463    1008 
   Size:        463     545
 
ApaLI G'TGCA_C

Cuts at:      0     725    1008 
   Size:        725     283
 
ApoI r'AATT_y

Cuts at:      0     335    1008 
   Size:        335     673
 
AseI AT'TA_AT

Cuts at:      0     145    1008 
   Size:        145     863
 
BclI T'GATC_A

Cuts at:      0     768    1008 
   Size:        768     240
 
BfrBI ATG'CAT

Cuts at:      0     544    1008 
   Size:        544     464
 
Bme1580I G_kGCm'C

Cuts at:      0     154     729    1008 
   Size:        154     575     279

  Fragments arranged by size: 

                 575     279     154
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34     236    1008 
   Size:         34     202     772

  Fragments arranged by size: 

                 772     202      34
 
BsiHKAI G_wGCw'C

Cuts at:      0     729    1004    1008 
   Size:        729     275       4

  Fragments arranged by size: 

                 729     275       4
 
Bsp1286I G_dGCh'C

Cuts at:      0     154     729    1004    1008 
   Size:        154     575     275       4

  Fragments arranged by size: 

                 575     275     154       4
 
BsrDI GCAATG_nn'

Cuts at:      0     644    1008 
   Size:        644     364
 
BstBI TT'CG_AA

Cuts at:      0     816    1008 
   Size:        816     192
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     708     786    1008 
   Size:        708      78     222

  Fragments arranged by size: 

                 708     222      78
 
BstZ17I GTA'TAC

Cuts at:      0     872    1008 
   Size:        872     136
 
Bsu36I CC'TnA_GG

Cuts at:      0      83    1008 
   Size:         83     925
 
BtgI C'CryG_G

Cuts at:      0     971    1008 
   Size:        971      37
 
BtsI GCAGTG_nn'

Cuts at:      0     531    1008 
   Size:        531     477
 
EaeI y'GGCC_r

Cuts at:      0     897    1008 
   Size:        897     111
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     463    1008 
   Size:        463     545
 
EcoO109I rG'GnC_Cy

Cuts at:      0     571    1008 
   Size:        571     437
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     760     792    1008 
   Size:        760      32     216

  Fragments arranged by size: 

                 760     216      32
 
MfeI C'AATT_G

Cuts at:      0     644    1008 
   Size:        644     364
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     250    1008 
   Size:        250     758
 
MscI TGG'CCA

Cuts at:      0     899    1008 
   Size:        899     109
 
MslI CAynn'nnrTG

Cuts at:      0     706    1008 
   Size:        706     302
 
NcoI C'CATG_G

Cuts at:      0     971    1008 
   Size:        971      37
 
NsiI A_TGCA'T

Cuts at:      0     546    1008 
   Size:        546     462
 
PstI C_TGCA'G

Cuts at:      0     470     538    1008 
   Size:        470      68     470

  Fragments arranged by size: 

                 470     470      68
 
SfcI C'TryA_G

Cuts at:      0     466     534    1008 
   Size:        466      68     474

  Fragments arranged by size: 

                 474     466      68
 
SpeI A'CTAG_T

Cuts at:      0     279    1008 
   Size:        279     729
 
StuI AGG'CCT

Cuts at:      0     693    1008 
   Size:        693     315
 
StyI C'CwwG_G

Cuts at:      0     971    1008 
   Size:        971      37
 
XmnI GAAnn'nnTTC

Cuts at:      0     812    1008 
   Size:        812     196
 

 Enzymes that do cut:

     AccI     AcuI    ApaLI     ApoI     AseI     BclI    BfrBI Bme1580I
     BsgI  BsiHKAI Bsp1286I    BsrDI    BstBI    BstXI  BstZ17I   Bsu36I
     BtgI     BtsI     EaeI  Eco57MI EcoO109I    Hin4I     MfeI     MmeI
     MscI     MslI     NcoI     NsiI     PstI     SfcI     SpeI     StuI
     StyI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     AscI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BglI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI   BstEII    BstYI    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MluI   MspA1I     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SphI     SrfI     SspI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI