Restriction map for
pF1KB7661
(Linear) (Six-base) MAPSORT of: KIBB7661.seq Check: 8204 from: 1 to: 1005
KIBB7661.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 351 1005
Size: 351 654
AccI GT'mk_AC
Cuts at: 0 983 1005
Size: 983 22
AfeI AGC'GCT
Cuts at: 0 580 1005
Size: 580 425
AhdI GACnn_n'nnGTC
Cuts at: 0 66 1005
Size: 66 939
AleI CACnn'nnGTG
Cuts at: 0 677 1005
Size: 677 328
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 553 585 1005
Size: 553 32 420
Fragments arranged by size:
553 420 32
AlwNI CAG_nnn'CTG
Cuts at: 0 162 215 531 1005
Size: 162 53 316 474
Fragments arranged by size:
474 316 162 53
ApaLI G'TGCA_C
Cuts at: 0 994 1005
Size: 994 11
AvaI C'yCGr_G
Cuts at: 0 15 112 151 1005
Size: 15 97 39 854
Fragments arranged by size:
854 97 39 15
BanI G'GyrC_C
Cuts at: 0 321 1005
Size: 321 684
BanII G_rGCy'C
Cuts at: 0 454 1005
Size: 454 551
BciVI GTATCCnnnnn_n'
Cuts at: 0 42 1005
Size: 42 963
BglI GCCn_nnn'nGGC
Cuts at: 0 235 330 859 1005
Size: 235 95 529 146
Fragments arranged by size:
529 235 146 95
BlpI GC'TnA_GC
Cuts at: 0 913 1005
Size: 913 92
Bme1580I G_kGCm'C
Cuts at: 0 280 326 998 1005
Size: 280 46 672 7
Fragments arranged by size:
672 280 46 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 128 1005
Size: 128 877
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 366 738 1005
Size: 366 372 267
Fragments arranged by size:
372 366 267
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 147 376 468 1005
Size: 147 229 92 537
Fragments arranged by size:
537 229 147 92
Bpu10I CC'TnA_GC
Cuts at: 0 269 1005
Size: 269 736
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 748 763 1005
Size: 376 372 15 242
Fragments arranged by size:
376 372 242 15
BsaI GGTCTCn'nnnn_
Cuts at: 0 115 1005
Size: 115 890
BsaWI w'CCGG_w
Cuts at: 0 953 1005
Size: 953 52
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 120 150 553 583 1005
Size: 120 30 403 30 422
Fragments arranged by size:
422 403 120 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 265 277 358 577 709 1005
Size: 265 12 81 219 132 296
Fragments arranged by size:
296 265 219 132 81 12
BseYI C'CCAG_C
Cuts at: 0 37 215 300 454 971 1005
Size: 37 178 85 154 517 34
Fragments arranged by size:
517 178 154 85 37 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 484 589 1005
Size: 385 99 105 416
Fragments arranged by size:
416 385 105 99
BsiHKAI G_wGCw'C
Cuts at: 0 350 392 491 998 1005
Size: 350 42 99 507 7
Fragments arranged by size:
507 350 99 42 7
BsmBI CGTCTCn'nnnn_
Cuts at: 0 111 397 1005
Size: 111 286 608
Fragments arranged by size:
608 286 111
Bsp1286I G_dGCh'C
Cuts at: 0 280 326 350 392 454 491 998 1005
Size: 280 46 24 42 62 37 507 7
Fragments arranged by size:
507 280 62 46 42 37 24 7
BspEI T'CCGG_A
Cuts at: 0 953 1005
Size: 953 52
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 351 1005
Size: 351 654
BsrBI CCG'CTC
Cuts at: 0 651 920 1005
Size: 651 269 85
Fragments arranged by size:
651 269 85
BsrDI GCAATG_nn'
Cuts at: 0 465 1005
Size: 465 540
BsrFI r'CCGG_y
Cuts at: 0 497 1005
Size: 497 508
BstXI CCAn_nnnn'nTGG
Cuts at: 0 378 793 1005
Size: 378 415 212
Fragments arranged by size:
415 378 212
BstYI r'GATC_y
Cuts at: 0 435 1005
Size: 435 570
Bsu36I CC'TnA_GG
Cuts at: 0 203 260 1005
Size: 203 57 745
Fragments arranged by size:
745 203 57
BtgI C'CryG_G
Cuts at: 0 371 440 509 1005
Size: 371 69 69 496
Fragments arranged by size:
496 371 69 69
BtsI GCAGTG_nn'
Cuts at: 0 110 280 1005
Size: 110 170 725
Fragments arranged by size:
725 170 110
EaeI y'GGCC_r
Cuts at: 0 795 1005
Size: 795 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 156 562 916 1005
Size: 156 406 354 89
Fragments arranged by size:
406 354 156 89
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 147 376 468 1005
Size: 147 229 92 537
Fragments arranged by size:
537 229 147 92
EcoO109I rG'GnC_Cy
Cuts at: 0 159 228 1005
Size: 159 69 777
Fragments arranged by size:
777 159 69
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 849 1005
Size: 849 156
HaeII r_GCGC'y
Cuts at: 0 11 582 978 1005
Size: 11 571 396 27
Fragments arranged by size:
571 396 27 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 52 84 120 152 1005
Size: 52 32 36 32 853
Fragments arranged by size:
853 52 36 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 539 1005
Size: 539 466
MslI CAynn'nnrTG
Cuts at: 0 376 677 791 1005
Size: 376 301 114 214
Fragments arranged by size:
376 301 214 114
MspA1I CmG'CkG
Cuts at: 0 97 215 304 634 1005
Size: 97 118 89 330 371
Fragments arranged by size:
371 330 118 97 89
NcoI C'CATG_G
Cuts at: 0 371 440 509 1005
Size: 371 69 69 496
Fragments arranged by size:
496 371 69 69
NspI r_CATG'y
Cuts at: 0 195 1005
Size: 195 810
PasI CC'CwG_GG
Cuts at: 0 232 588 1005
Size: 232 356 417
Fragments arranged by size:
417 356 232
PflMI CCAn_nnn'nTGG
Cuts at: 0 446 531 1005
Size: 446 85 474
Fragments arranged by size:
474 446 85
PfoI T'CCnGG_A
Cuts at: 0 524 1005
Size: 524 481
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 553 585 1005
Size: 553 32 420
Fragments arranged by size:
553 420 32
PshAI GACnn'nnGTC
Cuts at: 0 128 1005
Size: 128 877
PvuII CAG'CTG
Cuts at: 0 215 1005
Size: 215 790
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 235 1005
Size: 235 770
SmaI CCC'GGG
Cuts at: 0 17 1005
Size: 17 988
SmlI C'TyrA_G
Cuts at: 0 112 391 763 778 1005
Size: 112 279 372 15 227
Fragments arranged by size:
372 279 227 112 15
StuI AGG'CCT
Cuts at: 0 520 1005
Size: 520 485
StyI C'CwwG_G
Cuts at: 0 224 338 371 440 509 1005
Size: 224 114 33 69 69 496
Fragments arranged by size:
496 224 114 69 69 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 580 721 1005
Size: 580 141 284
Fragments arranged by size:
580 284 141
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 420 452 1005
Size: 420 32 553
Fragments arranged by size:
553 420 32
XhoI C'TCGA_G
Cuts at: 0 112 1005
Size: 112 893
XmaI C'CCGG_G
Cuts at: 0 15 1005
Size: 15 990
Enzymes that do cut:
AarI AccI AfeI AhdI AleI AloI AlwNI ApaLI
AvaI BanI BanII BciVI BglI BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BstXI BstYI Bsu36I BtgI BtsI EaeI
EciI Eco57MI EcoO109I FalI HaeII Hin4I MmeI MslI
MspA1I NcoI NspI PasI PflMI PfoI PpiI PshAI
PvuII SfiI SmaI SmlI StuI StyI TaqII TstI
XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AflII AflIII AgeI AlfI
ApaI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbsI BbvCI BcgI BclI BfrBI BglII BmgBI
BmtI BsaAI BsaBI BsaHI BsiEI BsiWI BsmI BspHI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EarI EcoICRI
EcoNI EcoRI EcoRV FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI MscI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PmeI PmlI PpuMI PsiI PspOMI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI TatI Tth111I XbaI XcmI
XmnI ZraI