Restriction map for
pF1KB7660
(Linear) (Six-base) MAPSORT of: KIBB7660.seq Check: 4687 from: 1 to: 1185
KIBB7660.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 750 1185
Size: 750 435
AccI GT'mk_AC
Cuts at: 0 713 1185
Size: 713 472
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1051 1185
Size: 1051 134
AflIII A'CryG_T
Cuts at: 0 271 1185
Size: 271 914
AgeI A'CCGG_T
Cuts at: 0 598 1185
Size: 598 587
AlwNI CAG_nnn'CTG
Cuts at: 0 592 1185
Size: 592 593
ApaI G_GGCC'C
Cuts at: 0 64 116 201 442 1160 1178 1185
Size: 64 52 85 241 718 18 7
Fragments arranged by size:
718 241 85 64 52 18 7
ApoI r'AATT_y
Cuts at: 0 332 1185
Size: 332 853
AvaI C'yCGr_G
Cuts at: 0 620 1137 1185
Size: 620 517 48
Fragments arranged by size:
620 517 48
BamHI G'GATC_C
Cuts at: 0 84 1185
Size: 84 1101
BanI G'GyrC_C
Cuts at: 0 166 352 406 522 762 1185
Size: 166 186 54 116 240 423
Fragments arranged by size:
423 240 186 166 116 54
BanII G_rGCy'C
Cuts at: 0 64 73 116 143 201 305 442 673
Size: 64 9 43 27 58 104 137 231
Cuts at: 673 1160 1178 1185
Size: 487 18 7
Fragments arranged by size:
487 231 137 104 64 58 43 27
18 9 7
BbeI G_GCGC'C
Cuts at: 0 356 410 1185
Size: 356 54 775
Fragments arranged by size:
775 356 54
BglI GCCn_nnn'nGGC
Cuts at: 0 947 1173 1185
Size: 947 226 12
Fragments arranged by size:
947 226 12
BglII A'GATC_T
Cuts at: 0 364 1185
Size: 364 821
BlpI GC'TnA_GC
Cuts at: 0 1124 1185
Size: 1124 61
Bme1580I G_kGCm'C
Cuts at: 0 64 116 169 201 442 527 616 767
Size: 64 52 53 32 241 85 89 151
Cuts at: 767 1160 1178 1185
Size: 393 18 7
Fragments arranged by size:
393 241 151 89 85 64 53 52
32 18 7
BmgBI CAC'GTC
Cuts at: 0 272 1185
Size: 272 913
BmrI ACTGGGnnnn_n'
Cuts at: 0 509 517 1185
Size: 509 8 668
Fragments arranged by size:
668 509 8
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 346 728 821 1093 1185
Size: 319 27 382 93 272 92
Fragments arranged by size:
382 319 272 93 92 27
Bpu10I CC'TnA_GC
Cuts at: 0 672 1185
Size: 672 513
BsaI GGTCTCn'nnnn_
Cuts at: 0 287 589 1185
Size: 287 302 596
Fragments arranged by size:
596 302 287
BsaHI Gr'CG_yC
Cuts at: 0 353 407 1185
Size: 353 54 778
Fragments arranged by size:
778 353 54
BsaWI w'CCGG_w
Cuts at: 0 598 934 1185
Size: 598 336 251
Fragments arranged by size:
598 336 251
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 555 1030 1125 1185
Size: 555 475 95 60
Fragments arranged by size:
555 475 95 60
BseYI C'CCAG_C
Cuts at: 0 142 526 572 664 784 792 1185
Size: 142 384 46 92 120 8 393
Fragments arranged by size:
393 384 142 120 92 46 8
BsiEI CG_ry'CG
Cuts at: 0 264 1185
Size: 264 921
BsiHKAI G_wGCw'C
Cuts at: 0 744 1185
Size: 744 441
Bsp1286I G_dGCh'C
Cuts at: 0 64 73 116 143 169 201 305 442
Size: 64 9 43 27 26 32 104 137
Cuts at: 442 527 616 673 744 767 1160 1178 1185
Size: 85 89 57 71 23 393 18 7
Fragments arranged by size:
393 137 104 89 85 71 64 57
43 32 27 26 23 18 9 7
BspEI T'CCGG_A
Cuts at: 0 934 1185
Size: 934 251
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 750 1185
Size: 750 435
BsrBI CCG'CTC
Cuts at: 0 5 491 1059 1185
Size: 5 486 568 126
Fragments arranged by size:
568 486 126 5
BsrFI r'CCGG_y
Cuts at: 0 598 1185
Size: 598 587
BssHII G'CGCG_C
Cuts at: 0 1095 1185
Size: 1095 90
BstEII G'GTnAC_C
Cuts at: 0 904 1185
Size: 904 281
BstXI CCAn_nnnn'nTGG
Cuts at: 0 981 1185
Size: 981 204
BstYI r'GATC_y
Cuts at: 0 84 364 1040 1185
Size: 84 280 676 145
Fragments arranged by size:
676 280 145 84
Bsu36I CC'TnA_GG
Cuts at: 0 345 482 1185
Size: 345 137 703
Fragments arranged by size:
703 345 137
BtgI C'CryG_G
Cuts at: 0 47 234 258 974 1147 1185
Size: 47 187 24 716 173 38
Fragments arranged by size:
716 187 173 47 38 24
EaeI y'GGCC_r
Cuts at: 0 389 893 1103 1185
Size: 389 504 210 82
Fragments arranged by size:
504 389 210 82
EarI CTCTTCn'nnn_
Cuts at: 0 13 743 1185
Size: 13 730 442
Fragments arranged by size:
730 442 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 332 1069 1185
Size: 332 737 116
Fragments arranged by size:
737 332 116
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 346 728 821 1051 1093 1185
Size: 319 27 382 93 230 42 92
Fragments arranged by size:
382 319 230 93 92 42 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 433 645 1185
Size: 433 212 540
Fragments arranged by size:
540 433 212
EcoO109I rG'GnC_Cy
Cuts at: 0 60 61 113 197 198 341 438 439
Size: 60 1 52 84 1 143 97 1
Cuts at: 439 683 1143 1157 1175 1185
Size: 244 460 14 18 10
Fragments arranged by size:
460 244 143 97 84 60 52 18
14 10 1 1 1
EcoRI G'AATT_C
Cuts at: 0 332 1185
Size: 332 853
HaeII r_GCGC'y
Cuts at: 0 356 410 582 1185
Size: 356 54 172 603
Fragments arranged by size:
603 356 172 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 916 948 1185
Size: 916 32 237
Fragments arranged by size:
916 237 32
KasI G'GCGC_C
Cuts at: 0 352 406 1185
Size: 352 54 779
Fragments arranged by size:
779 352 54
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 362 1185
Size: 362 823
MscI TGG'CCA
Cuts at: 0 391 895 1105 1185
Size: 391 504 210 80
Fragments arranged by size:
504 391 210 80
MspA1I CmG'CkG
Cuts at: 0 260 516 1119 1128 1185
Size: 260 256 603 9 57
Fragments arranged by size:
603 260 256 57 9
NarI GG'CG_CC
Cuts at: 0 353 407 1185
Size: 353 54 778
Fragments arranged by size:
778 353 54
NcoI C'CATG_G
Cuts at: 0 974 1185
Size: 974 211
NspI r_CATG'y
Cuts at: 0 720 1185
Size: 720 465
PasI CC'CwG_GG
Cuts at: 0 64 73 1185
Size: 64 9 1112
Fragments arranged by size:
1112 64 9
PflMI CCAn_nnn'nTGG
Cuts at: 0 193 240 449 519 1153 1185
Size: 193 47 209 70 634 32
Fragments arranged by size:
634 209 193 70 47 32
PfoI T'CCnGG_A
Cuts at: 0 323 474 697 1010 1043 1185
Size: 323 151 223 313 33 142
Fragments arranged by size:
323 313 223 151 142 33
PpuMI rG'GwC_Cy
Cuts at: 0 683 1143 1185
Size: 683 460 42
Fragments arranged by size:
683 460 42
PspOMI G'GGCC_C
Cuts at: 0 60 112 197 438 1156 1174 1185
Size: 60 52 85 241 718 18 11
Fragments arranged by size:
718 241 85 60 52 18 11
PvuII CAG'CTG
Cuts at: 0 516 1119 1128 1185
Size: 516 603 9 57
Fragments arranged by size:
603 516 57 9
RsrII CG'GwC_CG
Cuts at: 0 1008 1185
Size: 1008 177
SacII CC_GC'GG
Cuts at: 0 261 1185
Size: 261 924
SanDI GG'GwC_CC
Cuts at: 0 1143 1185
Size: 1143 42
SfcI C'TryA_G
Cuts at: 0 710 1185
Size: 710 475
SfoI GGC'GCC
Cuts at: 0 354 408 1185
Size: 354 54 777
Fragments arranged by size:
777 354 54
SmaI CCC'GGG
Cuts at: 0 622 1185
Size: 622 563
StuI AGG'CCT
Cuts at: 0 428 1185
Size: 428 757
StyI C'CwwG_G
Cuts at: 0 536 974 1185
Size: 536 438 211
Fragments arranged by size:
536 438 211
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 727 823 1185
Size: 727 96 362
Fragments arranged by size:
727 362 96
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 384 399 1185
Size: 384 15 786
Fragments arranged by size:
786 384 15
XmaI C'CCGG_G
Cuts at: 0 620 1185
Size: 620 565
Enzymes that do cut:
AarI AccI AcuI AflIII AgeI AlwNI ApaI ApoI
AvaI BamHI BanI BanII BbeI BglI BglII BlpI
Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI
BsrFI BssHII BstEII BstXI BstYI Bsu36I BtgI EaeI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I
KasI MmeI MscI MspA1I NarI NcoI NspI PasI
PflMI PfoI PpuMI PspOMI PvuII RsrII SacII SanDI
SfcI SfoI SmaI StuI StyI TaqII XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AhdI AleI AlfI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BbsI
BbvCI BcgI BciVI BclI BfrBI BmtI BplI BpuEI
BsaAI BsaBI BsaXI BsgI BsiWI BsmI BsmBI BspHI
BsrDI BsrGI BssSI BstAPI BstBI BstZ17I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI MslI NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PstI PvuI SacI SalI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TatI TstI Tth111I
XbaI XhoI XmnI ZraI