Restriction map for
pF1KB7656
(Linear) (Six-base) MAPSORT of: KIBB7656.seq Check: 1980 from: 1 to: 1182
KIBB7656.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 1182
Size: 439 743
AlwNI CAG_nnn'CTG
Cuts at: 0 552 1182
Size: 552 630
ApoI r'AATT_y
Cuts at: 0 205 285 424 1182
Size: 205 80 139 758
Fragments arranged by size:
758 205 139 80
AvaI C'yCGr_G
Cuts at: 0 916 1138 1182
Size: 916 222 44
Fragments arranged by size:
916 222 44
BanI G'GyrC_C
Cuts at: 0 1151 1182
Size: 1151 31
BclI T'GATC_A
Cuts at: 0 464 1182
Size: 464 718
BglI GCCn_nnn'nGGC
Cuts at: 0 414 1175 1182
Size: 414 761 7
Fragments arranged by size:
761 414 7
BglII A'GATC_T
Cuts at: 0 102 1182
Size: 102 1080
BmrI ACTGGGnnnn_n'
Cuts at: 0 558 993 1182
Size: 558 435 189
Fragments arranged by size:
558 435 189
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 462 787 1182
Size: 462 325 395
Fragments arranged by size:
462 395 325
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 1182
Size: 127 1055
BsaI GGTCTCn'nnnn_
Cuts at: 0 608 1182
Size: 608 574
BsaAI yAC'GTr
Cuts at: 0 153 1182
Size: 153 1029
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1062 1182
Size: 1062 120
BspHI T'CATG_A
Cuts at: 0 956 1025 1182
Size: 956 69 157
Fragments arranged by size:
956 157 69
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 957 1182
Size: 957 225
BsrDI GCAATG_nn'
Cuts at: 0 313 1182
Size: 313 869
BsrGI T'GTAC_A
Cuts at: 0 741 1182
Size: 741 441
BssSI C'ACGA_G
Cuts at: 0 730 1182
Size: 730 452
BstAPI GCAn_nnn'nTGC
Cuts at: 0 315 1182
Size: 315 867
BstBI TT'CG_AA
Cuts at: 0 255 1182
Size: 255 927
BstYI r'GATC_y
Cuts at: 0 102 1182
Size: 102 1080
BtsI GCAGTG_nn'
Cuts at: 0 11 726 1182
Size: 11 715 456
Fragments arranged by size:
715 456 11
DraI TTT'AAA
Cuts at: 0 48 1127 1182
Size: 48 1079 55
Fragments arranged by size:
1079 55 48
DraIII CAC_nnn'GTG
Cuts at: 0 750 1182
Size: 750 432
EaeI y'GGCC_r
Cuts at: 0 1167 1176 1182
Size: 1167 9 6
Fragments arranged by size:
1167 9 6
EarI CTCTTCn'nnn_
Cuts at: 0 15 1024 1064 1070 1076 1088 1182
Size: 15 1009 40 6 6 12 94
Fragments arranged by size:
1009 94 40 15 12 6 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 408 1182
Size: 408 774
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 462 787 1182
Size: 439 23 325 395
Fragments arranged by size:
439 395 325 23
EcoO109I rG'GnC_Cy
Cuts at: 0 725 1182
Size: 725 457
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 369 1182
Size: 369 813
HaeII r_GCGC'y
Cuts at: 0 366 1182
Size: 366 816
HindIII A'AGCT_T
Cuts at: 0 434 1182
Size: 434 748
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 109 1182
Size: 44 65 1073
Fragments arranged by size:
1073 65 44
MscI TGG'CCA
Cuts at: 0 1169 1178 1182
Size: 1169 9 4
Fragments arranged by size:
1169 9 4
NdeI CA'TA_TG
Cuts at: 0 165 1182
Size: 165 1017
PflMI CCAn_nnn'nTGG
Cuts at: 0 669 1182
Size: 669 513
PfoI T'CCnGG_A
Cuts at: 0 428 1037 1182
Size: 428 609 145
Fragments arranged by size:
609 428 145
PpuMI rG'GwC_Cy
Cuts at: 0 725 1182
Size: 725 457
PspXI vC'TCGA_Gb
Cuts at: 0 916 1182
Size: 916 266
SanDI GG'GwC_CC
Cuts at: 0 725 1182
Size: 725 457
SapI GCTCTTCn'nnn_
Cuts at: 0 15 1024 1182
Size: 15 1009 158
Fragments arranged by size:
1009 158 15
ScaI AGT'ACT
Cuts at: 0 568 1182
Size: 568 614
SfcI C'TryA_G
Cuts at: 0 949 1182
Size: 949 233
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1175 1182
Size: 1175 7
SmlI C'TyrA_G
Cuts at: 0 106 916 1182
Size: 106 810 266
Fragments arranged by size:
810 266 106
StuI AGG'CCT
Cuts at: 0 408 1182
Size: 408 774
StyI C'CwwG_G
Cuts at: 0 502 1182
Size: 502 680
TatI w'GTAC_w
Cuts at: 0 566 741 1182
Size: 566 175 441
Fragments arranged by size:
566 441 175
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 92 566 1182
Size: 92 474 616
Fragments arranged by size:
616 474 92
XhoI C'TCGA_G
Cuts at: 0 916 1182
Size: 916 266
XmnI GAAnn'nnTTC
Cuts at: 0 289 424 1182
Size: 289 135 758
Fragments arranged by size:
758 289 135
Enzymes that do cut:
AcuI AlwNI ApoI AvaI BanI BclI BglI BglII
BmrI BpmI BpuEI BsaI BsaAI BseRI BspHI BspMI
BsrDI BsrGI BssSI BstAPI BstBI BstYI BtsI DraI
DraIII EaeI EarI EciI Eco57MI EcoO109I FalI HaeII
HindIII MmeI MscI NdeI PflMI PfoI PpuMI PspXI
SanDI SapI ScaI SfcI SfiI SmlI StuI StyI
TatI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BanII BbeI BbsI
BbvCI BcgI BciVI BfrBI BlpI Bme1580I BmgBI BmtI
BplI Bpu10I BsaBI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BsrBI
BsrFI BssHII BstEII BstXI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DrdI EagI EcoICRI EcoNI EcoRI EcoRV
FseI FspI FspAI Hin4I HincII HpaI KasI KpnI
MfeI MluI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SbfI SexAI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TaqII TstI Tth111I XbaI XmaI ZraI