Restriction map for
pF1KB7650
(Linear) (Six-base) MAPSORT of: KIBB7650.seq Check: 7621 from: 1 to: 1176
KIBB7650.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1096 1176
Size: 1096 80
AccI GT'mk_AC
Cuts at: 0 735 1176
Size: 735 441
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 810 1176
Size: 810 366
AfeI AGC'GCT
Cuts at: 0 58 1176
Size: 58 1118
AlwNI CAG_nnn'CTG
Cuts at: 0 966 1163 1176
Size: 966 197 13
Fragments arranged by size:
966 197 13
ApaLI G'TGCA_C
Cuts at: 0 215 1176
Size: 215 961
ApoI r'AATT_y
Cuts at: 0 418 1176
Size: 418 758
AvaI C'yCGr_G
Cuts at: 0 70 1176
Size: 70 1106
BanI G'GyrC_C
Cuts at: 0 1126 1176
Size: 1126 50
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 156 190 1176
Size: 156 34 986
Fragments arranged by size:
986 156 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 314 1176
Size: 314 862
BclI T'GATC_A
Cuts at: 0 644 725 1016 1176
Size: 644 81 291 160
Fragments arranged by size:
644 291 160 81
BglI GCCn_nnn'nGGC
Cuts at: 0 775 1176
Size: 775 401
Bme1580I G_kGCm'C
Cuts at: 0 219 1176
Size: 219 957
BmrI ACTGGGnnnn_n'
Cuts at: 0 1173 1176
Size: 1173 3
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1080 1176
Size: 1080 96
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 343 781 1101 1176
Size: 115 228 438 320 75
Fragments arranged by size:
438 320 228 115 75
Bpu10I CC'TnA_GC
Cuts at: 0 25 1176
Size: 25 1151
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 316 346 1099 1129 1176
Size: 316 30 753 30 47
Fragments arranged by size:
753 316 47 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 925 1176
Size: 925 251
BseYI C'CCAG_C
Cuts at: 0 83 1176
Size: 83 1093
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1074 1176
Size: 1074 102
BsiEI CG_ry'CG
Cuts at: 0 107 132 1176
Size: 107 25 1044
Fragments arranged by size:
1044 107 25
BsiHKAI G_wGCw'C
Cuts at: 0 219 1176
Size: 219 957
Bsp1286I G_dGCh'C
Cuts at: 0 219 1176
Size: 219 957
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1096 1176
Size: 1096 80
BsrDI GCAATG_nn'
Cuts at: 0 1128 1176
Size: 1128 48
BsrFI r'CCGG_y
Cuts at: 0 102 131 1176
Size: 102 29 1045
Fragments arranged by size:
1045 102 29
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1000 1176
Size: 1000 176
BstEII G'GTnAC_C
Cuts at: 0 118 666 1176
Size: 118 548 510
Fragments arranged by size:
548 510 118
BtgI C'CryG_G
Cuts at: 0 126 1176
Size: 126 1050
BtsI GCAGTG_nn'
Cuts at: 0 1053 1176
Size: 1053 123
EaeI y'GGCC_r
Cuts at: 0 104 129 776 1176
Size: 104 25 647 400
Fragments arranged by size:
647 400 104 25
EagI C'GGCC_G
Cuts at: 0 104 129 1176
Size: 104 25 1047
Fragments arranged by size:
1047 104 25
EarI CTCTTCn'nnn_
Cuts at: 0 193 1166 1176
Size: 193 973 10
Fragments arranged by size:
973 193 10
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 343 781 810 1101 1176
Size: 115 228 438 29 291 75
Fragments arranged by size:
438 291 228 115 75 29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 277 1017 1176
Size: 277 740 159
Fragments arranged by size:
740 277 159
EcoO109I rG'GnC_Cy
Cuts at: 0 19 327 1176
Size: 19 308 849
Fragments arranged by size:
849 308 19
FseI GG_CCGG'CC
Cuts at: 0 106 1176
Size: 106 1070
HaeII r_GCGC'y
Cuts at: 0 60 1176
Size: 60 1116
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 316 348 717 749 842 874 1099 1131
Size: 316 32 369 32 93 32 225 32
Cuts at: 1131 1176
Size: 45
Fragments arranged by size:
369 316 225 93 45 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 27 1176
Size: 27 1149
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 537 1176
Size: 537 639
MscI TGG'CCA
Cuts at: 0 778 1176
Size: 778 398
MslI CAynn'nnrTG
Cuts at: 0 652 1176
Size: 652 524
MspA1I CmG'CkG
Cuts at: 0 128 156 342 798 1176
Size: 128 28 186 456 378
Fragments arranged by size:
456 378 186 128 28
NaeI GCC'GGC
Cuts at: 0 104 133 1176
Size: 104 29 1043
Fragments arranged by size:
1043 104 29
NgoMIV G'CCGG_C
Cuts at: 0 102 131 1176
Size: 102 29 1045
Fragments arranged by size:
1045 102 29
NspI r_CATG'y
Cuts at: 0 435 973 1176
Size: 435 538 203
Fragments arranged by size:
538 435 203
PpuMI rG'GwC_Cy
Cuts at: 0 19 1176
Size: 19 1157
PshAI GACnn'nnGTC
Cuts at: 0 242 1176
Size: 242 934
PvuII CAG'CTG
Cuts at: 0 342 1176
Size: 342 834
SacII CC_GC'GG
Cuts at: 0 129 1176
Size: 129 1047
SanDI GG'GwC_CC
Cuts at: 0 19 1176
Size: 19 1157
SapI GCTCTTCn'nnn_
Cuts at: 0 193 1176
Size: 193 983
SfcI C'TryA_G
Cuts at: 0 958 1176
Size: 958 218
StyI C'CwwG_G
Cuts at: 0 496 1176
Size: 496 680
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1083 1115 1176
Size: 1083 32 61
Fragments arranged by size:
1083 61 32
Enzymes that do cut:
AarI AccI AcuI AfeI AlwNI ApaLI ApoI AvaI
BanI BcgI BciVI BclI BglI Bme1580I BmrI BplI
BpmI Bpu10I BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspMI BsrDI BsrFI BstAPI BstEII BtgI BtsI
EaeI EagI EarI Eco57MI EcoNI EcoO109I FseI HaeII
Hin4I HindIII MmeI MscI MslI MspA1I NaeI NgoMIV
NspI PpuMI PshAI PvuII SacII SanDI SapI SfcI
StyI TstI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BanII BbeI BbsI BbvCI BfrBI BglII BlpI
BmgBI BmtI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI BssHII
BssSI BstBI BstXI BstYI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EciI EcoICRI EcoRI EcoRV
FalI FspI FspAI HincII HpaI KasI KpnI MfeI
MluI NarI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI
SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TatI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI