Restriction map for
pF1KB7646
(Linear) (Six-base) MAPSORT of: KIBB7646.seq Check: 4875 from: 1 to: 1170
KIBB7646.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1099 1170
Size: 1099 71
AhdI GACnn_n'nnGTC
Cuts at: 0 1151 1170
Size: 1151 19
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 326 1170
Size: 326 844
AlwNI CAG_nnn'CTG
Cuts at: 0 807 984 1170
Size: 807 177 186
Fragments arranged by size:
807 186 177
BanII G_rGCy'C
Cuts at: 0 758 1170
Size: 758 412
BbsI GAAGACnn'nnnn_
Cuts at: 0 414 788 1022 1065 1084 1170
Size: 414 374 234 43 19 86
Fragments arranged by size:
414 374 234 86 43 19
BbvCI CC'TCA_GC
Cuts at: 0 1117 1170
Size: 1117 53
BciVI GTATCCnnnnn_n'
Cuts at: 0 523 1170
Size: 523 647
BglII A'GATC_T
Cuts at: 0 99 1170
Size: 99 1071
Bme1580I G_kGCm'C
Cuts at: 0 747 1125 1170
Size: 747 378 45
Fragments arranged by size:
747 378 45
BmrI ACTGGGnnnn_n'
Cuts at: 0 539 638 1170
Size: 539 99 532
Fragments arranged by size:
539 532 99
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 703 1170
Size: 703 467
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 401 1170
Size: 401 769
Bpu10I CC'TnA_GC
Cuts at: 0 1117 1170
Size: 1117 53
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 7 1065 1170
Size: 7 1058 105
Fragments arranged by size:
1058 105 7
BsaI GGTCTCn'nnnn_
Cuts at: 0 47 1159 1170
Size: 47 1112 11
Fragments arranged by size:
1112 47 11
BsaAI yAC'GTr
Cuts at: 0 67 1170
Size: 67 1103
BsaWI w'CCGG_w
Cuts at: 0 526 1170
Size: 526 644
BsiHKAI G_wGCw'C
Cuts at: 0 758 1170
Size: 758 412
Bsp1286I G_dGCh'C
Cuts at: 0 747 758 1125 1170
Size: 747 11 367 45
Fragments arranged by size:
747 367 45 11
BspEI T'CCGG_A
Cuts at: 0 526 1170
Size: 526 644
BspHI T'CATG_A
Cuts at: 0 389 1170
Size: 389 781
BsrFI r'CCGG_y
Cuts at: 0 1150 1170
Size: 1150 20
BstEII G'GTnAC_C
Cuts at: 0 550 796 819 1170
Size: 550 246 23 351
Fragments arranged by size:
550 351 246 23
BstYI r'GATC_y
Cuts at: 0 99 980 1170
Size: 99 881 190
Fragments arranged by size:
881 190 99
ClaI AT'CG_AT
Cuts at: 0 941 1170
Size: 941 229
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 528 563 1170
Size: 528 35 607
Fragments arranged by size:
607 528 35
DraIII CAC_nnn'GTG
Cuts at: 0 577 1170
Size: 577 593
DrdI GACnn_nn'nnGTC
Cuts at: 0 1069 1170
Size: 1069 101
EarI CTCTTCn'nnn_
Cuts at: 0 806 1170
Size: 806 364
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 440 735 1170
Size: 440 295 435
Fragments arranged by size:
440 435 295
EcoICRI GAG'CTC
Cuts at: 0 756 1170
Size: 756 414
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 401 1099 1170
Size: 401 698 71
Fragments arranged by size:
698 401 71
EcoNI CCTnn'n_nnAGG
Cuts at: 0 188 790 1047 1170
Size: 188 602 257 123
Fragments arranged by size:
602 257 188 123
EcoRV GAT'ATC
Cuts at: 0 939 1170
Size: 939 231
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 972 1004 1137 1169 1170
Size: 972 32 133 32 1
Fragments arranged by size:
972 133 32 32 1
MslI CAynn'nnrTG
Cuts at: 0 308 394 1170
Size: 308 86 776
Fragments arranged by size:
776 308 86
MspA1I CmG'CkG
Cuts at: 0 1116 1170
Size: 1116 54
NdeI CA'TA_TG
Cuts at: 0 123 1170
Size: 123 1047
NspI r_CATG'y
Cuts at: 0 307 492 501 1087 1170
Size: 307 185 9 586 83
Fragments arranged by size:
586 307 185 83 9
PflMI CCAn_nnn'nTGG
Cuts at: 0 577 807 1170
Size: 577 230 363
Fragments arranged by size:
577 363 230
PvuII CAG'CTG
Cuts at: 0 1116 1170
Size: 1116 54
SacI G_AGCT'C
Cuts at: 0 758 1170
Size: 758 412
SexAI A'CCwGG_T
Cuts at: 0 573 1170
Size: 573 597
SmlI C'TyrA_G
Cuts at: 0 22 1044 1170
Size: 22 1022 126
Fragments arranged by size:
1022 126 22
SpeI A'CTAG_T
Cuts at: 0 274 1170
Size: 274 896
SphI G_CATG'C
Cuts at: 0 501 1170
Size: 501 669
SspI AAT'ATT
Cuts at: 0 207 1170
Size: 207 963
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 371 1170
Size: 371 799
TatI w'GTAC_w
Cuts at: 0 41 1170
Size: 41 1129
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 624 656 1170
Size: 624 32 514
Fragments arranged by size:
624 514 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 218 1170
Size: 218 952
Enzymes that do cut:
AcuI AhdI AlfI AlwNI BanII BbsI BbvCI BciVI
BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaWI BsiHKAI Bsp1286I BspEI BspHI BsrFI BstEII
BstYI ClaI CspCI DraIII DrdI EarI EciI EcoICRI
Eco57MI EcoNI EcoRV Hin4I MslI MspA1I NdeI NspI
PflMI PvuII SacI SexAI SmlI SpeI SphI SspI
TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BcgI
BclI BfrBI BglI BlpI BmgBI BmtI BsaBI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiWI BsmI BsmBI
BspMI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstXI BstZ17I Bsu36I BtgI BtgZI BtsI DraI EaeI
EagI EcoO109I EcoRI FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI NaeI NarI NcoI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI StuI
StyI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI