Restriction map for
pF1KB7639
(Linear) (Six-base) MAPSORT of: KIBB7639.seq Check: 7867 from: 1 to: 978
KIBB7639.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AccI GT'mk_AC
Cuts at: 0 16 978
Size: 16 962
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 590 978
Size: 590 388
AflIII A'CryG_T
Cuts at: 0 689 978
Size: 689 289
AleI CACnn'nnGTG
Cuts at: 0 709 978
Size: 709 269
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 633 978
Size: 633 345
AlwNI CAG_nnn'CTG
Cuts at: 0 672 822 978
Size: 672 150 156
Fragments arranged by size:
672 156 150
ApaI G_GGCC'C
Cuts at: 0 186 525 978
Size: 186 339 453
Fragments arranged by size:
453 339 186
ApoI r'AATT_y
Cuts at: 0 420 978
Size: 420 558
AvaI C'yCGr_G
Cuts at: 0 179 978
Size: 179 799
BanI G'GyrC_C
Cuts at: 0 160 317 352 499 805 923 959 978
Size: 160 157 35 147 306 118 36 19
Fragments arranged by size:
306 160 157 147 118 36 35 19
BanII G_rGCy'C
Cuts at: 0 131 186 203 525 554 736 767 978
Size: 131 55 17 322 29 182 31 211
Fragments arranged by size:
322 211 182 131 55 31 29 17
BbeI G_GCGC'C
Cuts at: 0 164 321 809 927 963 978
Size: 164 157 488 118 36 15
Fragments arranged by size:
488 164 157 118 36 15
BlpI GC'TnA_GC
Cuts at: 0 174 978
Size: 174 804
Bme1580I G_kGCm'C
Cuts at: 0 186 525 978
Size: 186 339 453
Fragments arranged by size:
453 339 186
BmgBI CAC'GTC
Cuts at: 0 690 978
Size: 690 288
BmrI ACTGGGnnnn_n'
Cuts at: 0 485 978
Size: 485 493
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 465 978
Size: 465 513
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 229 235 317 342 978
Size: 145 84 6 82 25 636
Fragments arranged by size:
636 145 84 82 25 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 705 978
Size: 705 273
BsaHI Gr'CG_yC
Cuts at: 0 161 318 779 806 924 960 978
Size: 161 157 461 27 118 36 18
Fragments arranged by size:
461 161 157 118 36 27 18
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 31 61 978
Size: 31 30 917
Fragments arranged by size:
917 31 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 52 958 978
Size: 52 906 20
Fragments arranged by size:
906 52 20
BseYI C'CCAG_C
Cuts at: 0 32 255 525 536 978
Size: 32 223 270 11 442
Fragments arranged by size:
442 270 223 32 11
BsiHKAI G_wGCw'C
Cuts at: 0 131 203 767 978
Size: 131 72 564 211
Fragments arranged by size:
564 211 131 72
Bsp1286I G_dGCh'C
Cuts at: 0 131 186 203 525 554 736 767 978
Size: 131 55 17 322 29 182 31 211
Fragments arranged by size:
322 211 182 131 55 31 29 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 254 299 978
Size: 254 45 679
Fragments arranged by size:
679 254 45
BsrFI r'CCGG_y
Cuts at: 0 320 485 920 978
Size: 320 165 435 58
Fragments arranged by size:
435 320 165 58
BssHII G'CGCG_C
Cuts at: 0 307 978
Size: 307 671
BstAPI GCAn_nnn'nTGC
Cuts at: 0 267 822 978
Size: 267 555 156
Fragments arranged by size:
555 267 156
BtgI C'CryG_G
Cuts at: 0 222 978
Size: 222 756
BtsI GCAGTG_nn'
Cuts at: 0 235 716 978
Size: 235 481 262
Fragments arranged by size:
481 262 235
EaeI y'GGCC_r
Cuts at: 0 322 918 978
Size: 322 596 60
Fragments arranged by size:
596 322 60
EarI CTCTTCn'nnn_
Cuts at: 0 369 978
Size: 369 609
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 489 528 978
Size: 489 39 450
Fragments arranged by size:
489 450 39
EcoICRI GAG'CTC
Cuts at: 0 129 201 765 978
Size: 129 72 564 213
Fragments arranged by size:
564 213 129 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 229 235 317 342 590 978
Size: 145 84 6 82 25 248 388
Fragments arranged by size:
388 248 145 84 82 25 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 814 841 853 978
Size: 814 27 12 125
Fragments arranged by size:
814 125 27 12
EcoO109I rG'GnC_Cy
Cuts at: 0 521 522 726 819 846 978
Size: 521 1 204 93 27 132
Fragments arranged by size:
521 204 132 93 27 1
FseI GG_CCGG'CC
Cuts at: 0 489 978
Size: 489 489
HaeII r_GCGC'y
Cuts at: 0 7 164 222 321 673 809 927 963
Size: 7 157 58 99 352 136 118 36
Cuts at: 963 978
Size: 15
Fragments arranged by size:
352 157 136 118 99 58 36 15
7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 718 750 978
Size: 718 32 228
Fragments arranged by size:
718 228 32
HincII GTy'rAC
Cuts at: 0 17 978
Size: 17 961
KasI G'GCGC_C
Cuts at: 0 160 317 805 923 959 978
Size: 160 157 488 118 36 19
Fragments arranged by size:
488 160 157 118 36 19
MslI CAynn'nnrTG
Cuts at: 0 709 978
Size: 709 269
MspA1I CmG'CkG
Cuts at: 0 191 224 471 684 978
Size: 191 33 247 213 294
Fragments arranged by size:
294 247 213 191 33
NaeI GCC'GGC
Cuts at: 0 322 487 922 978
Size: 322 165 435 56
Fragments arranged by size:
435 322 165 56
NarI GG'CG_CC
Cuts at: 0 161 318 806 924 960 978
Size: 161 157 488 118 36 18
Fragments arranged by size:
488 161 157 118 36 18
NgoMIV G'CCGG_C
Cuts at: 0 320 485 920 978
Size: 320 165 435 58
Fragments arranged by size:
435 320 165 58
PflMI CCAn_nnn'nTGG
Cuts at: 0 672 978
Size: 672 306
PpuMI rG'GwC_Cy
Cuts at: 0 726 978
Size: 726 252
PspOMI G'GGCC_C
Cuts at: 0 182 521 978
Size: 182 339 457
Fragments arranged by size:
457 339 182
PstI C_TGCA'G
Cuts at: 0 818 978
Size: 818 160
PvuII CAG'CTG
Cuts at: 0 684 978
Size: 684 294
SacI G_AGCT'C
Cuts at: 0 131 203 767 978
Size: 131 72 564 211
Fragments arranged by size:
564 211 131 72
SacII CC_GC'GG
Cuts at: 0 225 978
Size: 225 753
SalI G'TCGA_C
Cuts at: 0 15 978
Size: 15 963
SapI GCTCTTCn'nnn_
Cuts at: 0 369 978
Size: 369 609
ScaI AGT'ACT
Cuts at: 0 616 978
Size: 616 362
SfcI C'TryA_G
Cuts at: 0 342 814 978
Size: 342 472 164
Fragments arranged by size:
472 342 164
SfoI GGC'GCC
Cuts at: 0 162 319 807 925 961 978
Size: 162 157 488 118 36 17
Fragments arranged by size:
488 162 157 118 36 17
SmaI CCC'GGG
Cuts at: 0 181 978
Size: 181 797
SmlI C'TyrA_G
Cuts at: 0 720 978
Size: 720 258
SrfI GCCC'GGGC
Cuts at: 0 181 978
Size: 181 797
StuI AGG'CCT
Cuts at: 0 973 978
Size: 973 5
StyI C'CwwG_G
Cuts at: 0 635 978
Size: 635 343
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 127 978
Size: 127 851
TatI w'GTAC_w
Cuts at: 0 614 978
Size: 614 364
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 31 63 978
Size: 31 32 915
Fragments arranged by size:
915 32 31
XmaI C'CCGG_G
Cuts at: 0 179 978
Size: 179 799
Enzymes that do cut:
AccI AcuI AflIII AleI AlfI AlwNI ApaI ApoI
AvaI BanI BanII BbeI BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaHI BsaXI BseRI BseYI BsiHKAI
Bsp1286I BspMI BsrFI BssHII BstAPI BtgI BtsI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FseI HaeII
Hin4I HincII KasI MslI MspA1I NaeI NarI NgoMIV
PflMI PpuMI PspOMI PstI PvuII SacI SacII SalI
SapI ScaI SfcI SfoI SmaI SmlI SrfI StuI
StyI TaqII TatI TstI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BmtI Bpu10I BsaI BsaAI BsaBI BsaWI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI
BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EcoRI EcoRV
FalI FspI FspAI HindIII HpaI KpnI MfeI MluI
MmeI MscI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SanDI SbfI
SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SspI
SwaI Tth111I XbaI XcmI XhoI XmnI ZraI