Restriction map for
pF1KB7638
(Linear) (Six-base) MAPSORT of: KIBB7638.seq Check: 534 from: 1 to: 1143
KIBB7638.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AccI GT'mk_AC
Cuts at: 0 672 1143
Size: 672 471
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 473 720 1143
Size: 473 247 423
Fragments arranged by size:
473 423 247
AhdI GACnn_n'nnGTC
Cuts at: 0 136 1143
Size: 136 1007
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 329 1143
Size: 329 814
AlwNI CAG_nnn'CTG
Cuts at: 0 881 1116 1143
Size: 881 235 27
Fragments arranged by size:
881 235 27
ApaI G_GGCC'C
Cuts at: 0 147 328 842 964 1143
Size: 147 181 514 122 179
Fragments arranged by size:
514 181 179 147 122
ApoI r'AATT_y
Cuts at: 0 419 1143
Size: 419 724
AvaI C'yCGr_G
Cuts at: 0 259 617 749 1143
Size: 259 358 132 394
Fragments arranged by size:
394 358 259 132
BamHI G'GATC_C
Cuts at: 0 664 1143
Size: 664 479
BanI G'GyrC_C
Cuts at: 0 1022 1143
Size: 1022 121
BanII G_rGCy'C
Cuts at: 0 147 328 694 750 842 964 987 1143
Size: 147 181 366 56 92 122 23 156
Fragments arranged by size:
366 181 156 147 122 92 56 23
BbvCI CC'TCA_GC
Cuts at: 0 686 1143
Size: 686 457
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 166 200 217 251 1143
Size: 166 34 17 34 892
Fragments arranged by size:
892 166 34 34 17
BglI GCCn_nnn'nGGC
Cuts at: 0 790 952 1143
Size: 790 162 191
Fragments arranged by size:
790 191 162
BlpI GC'TnA_GC
Cuts at: 0 361 1143
Size: 361 782
Bme1580I G_kGCm'C
Cuts at: 0 147 222 328 842 902 964 1143
Size: 147 75 106 514 60 62 179
Fragments arranged by size:
514 179 147 106 75 62 60
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 609 1143
Size: 609 534
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 490 538 678 1143
Size: 142 348 48 140 465
Fragments arranged by size:
465 348 142 140 48
Bpu10I CC'TnA_GC
Cuts at: 0 686 1143
Size: 686 457
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1020 1123 1143
Size: 1020 103 20
Fragments arranged by size:
1020 103 20
BsaI GGTCTCn'nnnn_
Cuts at: 0 400 1057 1143
Size: 400 657 86
Fragments arranged by size:
657 400 86
BsaAI yAC'GTr
Cuts at: 0 682 1143
Size: 682 461
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 488 511 518 541 1143
Size: 488 23 7 23 602
Fragments arranged by size:
602 488 23 23 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 145 472 733 736 875 1005 1143
Size: 145 327 261 3 139 130 138
Fragments arranged by size:
327 261 145 139 138 130 3
BseYI C'CCAG_C
Cuts at: 0 187 428 1074 1143
Size: 187 241 646 69
Fragments arranged by size:
646 241 187 69
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 1143
Size: 190 953
BsiHKAI G_wGCw'C
Cuts at: 0 854 1143
Size: 854 289
Bsp1286I G_dGCh'C
Cuts at: 0 147 222 328 694 750 842 854 902
Size: 147 75 106 366 56 92 12 48
Cuts at: 902 964 987 1143
Size: 62 23 156
Fragments arranged by size:
366 156 147 106 92 75 62 56
48 23 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1002 1143
Size: 1002 141
BsrBI CCG'CTC
Cuts at: 0 486 865 1143
Size: 486 379 278
Fragments arranged by size:
486 379 278
BsrFI r'CCGG_y
Cuts at: 0 53 1143
Size: 53 1090
BstAPI GCAn_nnn'nTGC
Cuts at: 0 307 1143
Size: 307 836
BstYI r'GATC_y
Cuts at: 0 664 1143
Size: 664 479
BtgI C'CryG_G
Cuts at: 0 68 288 380 935 1143
Size: 68 220 92 555 208
Fragments arranged by size:
555 220 208 92 68
BtsI GCAGTG_nn'
Cuts at: 0 202 301 1100 1143
Size: 202 99 799 43
Fragments arranged by size:
799 202 99 43
EaeI y'GGCC_r
Cuts at: 0 414 953 1143
Size: 414 539 190
Fragments arranged by size:
539 414 190
EarI CTCTTCn'nnn_
Cuts at: 0 449 739 758 842 1143
Size: 449 290 19 84 301
Fragments arranged by size:
449 301 290 84 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 366 1143
Size: 366 777
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 473 490 538 678 720 1143
Size: 142 331 17 48 140 42 423
Fragments arranged by size:
423 331 142 140 48 42 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 917 1108 1143
Size: 917 191 35
Fragments arranged by size:
917 191 35
EcoO109I rG'GnC_Cy
Cuts at: 0 150 324 433 924 960 961 1113 1134
Size: 150 174 109 491 36 1 152 21
Cuts at: 1134 1143
Size: 9
Fragments arranged by size:
491 174 152 150 109 36 21 9
1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 122 154 460 488 492 520 1143
Size: 122 32 306 28 4 28 623
Fragments arranged by size:
623 306 122 32 28 28 4
HincII GTy'rAC
Cuts at: 0 673 1143
Size: 673 470
MscI TGG'CCA
Cuts at: 0 416 955 1143
Size: 416 539 188
Fragments arranged by size:
539 416 188
MspA1I CmG'CkG
Cuts at: 0 357 368 729 1019 1143
Size: 357 11 361 290 124
Fragments arranged by size:
361 357 290 124 11
NaeI GCC'GGC
Cuts at: 0 55 1143
Size: 55 1088
NcoI C'CATG_G
Cuts at: 0 68 288 380 935 1143
Size: 68 220 92 555 208
Fragments arranged by size:
555 220 208 92 68
NgoMIV G'CCGG_C
Cuts at: 0 53 1143
Size: 53 1090
NspI r_CATG'y
Cuts at: 0 310 628 1143
Size: 310 318 515
Fragments arranged by size:
515 318 310
PasI CC'CwG_GG
Cuts at: 0 440 842 913 1143
Size: 440 402 71 230
Fragments arranged by size:
440 402 230 71
PflMI CCAn_nnn'nTGG
Cuts at: 0 423 1143
Size: 423 720
PmlI CAC'GTG
Cuts at: 0 682 1143
Size: 682 461
PpuMI rG'GwC_Cy
Cuts at: 0 433 924 1143
Size: 433 491 219
Fragments arranged by size:
491 433 219
PspOMI G'GGCC_C
Cuts at: 0 143 324 838 960 1143
Size: 143 181 514 122 183
Fragments arranged by size:
514 183 181 143 122
PstI C_TGCA'G
Cuts at: 0 129 945 1043 1143
Size: 129 816 98 100
Fragments arranged by size:
816 129 100 98
SalI G'TCGA_C
Cuts at: 0 671 1143
Size: 671 472
SanDI GG'GwC_CC
Cuts at: 0 433 924 1143
Size: 433 491 219
Fragments arranged by size:
491 433 219
SapI GCTCTTCn'nnn_
Cuts at: 0 739 758 842 1143
Size: 739 19 84 301
Fragments arranged by size:
739 301 84 19
SfcI C'TryA_G
Cuts at: 0 125 941 1039 1143
Size: 125 816 98 104
Fragments arranged by size:
816 125 104 98
SmaI CCC'GGG
Cuts at: 0 619 751 1143
Size: 619 132 392
Fragments arranged by size:
619 392 132
SmlI C'TyrA_G
Cuts at: 0 999 1138 1143
Size: 999 139 5
Fragments arranged by size:
999 139 5
SphI G_CATG'C
Cuts at: 0 310 1143
Size: 310 833
SrfI GCCC'GGGC
Cuts at: 0 751 1143
Size: 751 392
StuI AGG'CCT
Cuts at: 0 173 1143
Size: 173 970
StyI C'CwwG_G
Cuts at: 0 68 288 380 479 755 935 1143
Size: 68 220 92 99 276 180 208
Fragments arranged by size:
276 220 208 180 99 92 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 182 827 1143
Size: 182 645 316
Fragments arranged by size:
645 316 182
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 511 543 1143
Size: 511 32 600
Fragments arranged by size:
600 511 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 66 286 1143
Size: 66 220 857
Fragments arranged by size:
857 220 66
XmaI C'CCGG_G
Cuts at: 0 617 749 1143
Size: 617 132 394
Fragments arranged by size:
617 394 132
Enzymes that do cut:
AccI AcuI AhdI AlfI AlwNI ApaI ApoI AvaI
BamHI BanI BanII BbvCI BcgI BglI BlpI Bme1580I
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BstAPI
BstYI BtgI BtsI EaeI EarI EciI Eco57MI EcoNI
EcoO109I Hin4I HincII MscI MspA1I NaeI NcoI NgoMIV
NspI PasI PflMI PmlI PpuMI PspOMI PstI SalI
SanDI SapI SfcI SmaI SmlI SphI SrfI StuI
StyI TaqII TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AleI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BbeI BbsI BciVI BclI BfrBI BglII BmgBI BmrI
BmtI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrDI BsrGI BssHII BssSI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI
MmeI MslI NarI NdeI NheI NotI NruI NsiI
PacI PciI PfoI PmeI PpiI PshAI PsiI PspXI
PsrI PvuI PvuII RsrII SacI SacII SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SspI
SwaI TatI Tth111I XbaI XhoI XmnI ZraI