Restriction map for
pF1KB7630
(Linear) (Six-base) MAPSORT of: KIBB7630.seq Check: 1 from: 1 to: 1125
KIBB7630.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
Acc65I G'GTAC_C
Cuts at: 0 891 1125
Size: 891 234
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 471 1125
Size: 109 362 654
Fragments arranged by size:
654 362 109
AflII C'TTAA_G
Cuts at: 0 319 1125
Size: 319 806
AflIII A'CryG_T
Cuts at: 0 757 1125
Size: 757 368
AleI CACnn'nnGTG
Cuts at: 0 415 1125
Size: 415 710
AlwNI CAG_nnn'CTG
Cuts at: 0 1058 1125
Size: 1058 67
ApaI G_GGCC'C
Cuts at: 0 22 1125
Size: 22 1103
AvaI C'yCGr_G
Cuts at: 0 637 1125
Size: 637 488
BanI G'GyrC_C
Cuts at: 0 891 1125
Size: 891 234
BanII G_rGCy'C
Cuts at: 0 22 512 644 656 755 817 862 920
Size: 22 490 132 12 99 62 45 58
Cuts at: 920 1125
Size: 205
Fragments arranged by size:
490 205 132 99 62 58 45 22
12
BbvCI CC'TCA_GC
Cuts at: 0 682 1125
Size: 682 443
BciVI GTATCCnnnnn_n'
Cuts at: 0 44 1125
Size: 44 1081
BfrBI ATG'CAT
Cuts at: 0 166 537 1125
Size: 166 371 588
Fragments arranged by size:
588 371 166
Bme1580I G_kGCm'C
Cuts at: 0 22 1125
Size: 22 1103
BmgBI CAC'GTC
Cuts at: 0 479 760 1109 1125
Size: 479 281 349 16
Fragments arranged by size:
479 349 281 16
BmrI ACTGGGnnnn_n'
Cuts at: 0 978 1051 1125
Size: 978 73 74
Fragments arranged by size:
978 74 73
BmtI G_CTAG'C
Cuts at: 0 869 1125
Size: 869 256
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 241 463 867 994 1125
Size: 70 171 222 404 127 131
Fragments arranged by size:
404 222 171 131 127 70
Bpu10I CC'TnA_GC
Cuts at: 0 682 1125
Size: 682 443
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 685 1125
Size: 685 440
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 418 436 448 466 1125
Size: 418 18 12 18 659
Fragments arranged by size:
659 418 18 18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 154 1125
Size: 154 971
BseYI C'CCAG_C
Cuts at: 0 389 769 861 1125
Size: 389 380 92 264
Fragments arranged by size:
389 380 264 92
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1022 1125
Size: 1022 103
BsiHKAI G_wGCw'C
Cuts at: 0 512 656 817 1125
Size: 512 144 161 308
Fragments arranged by size:
512 308 161 144
BsmI GAATG_Cn'
Cuts at: 0 166 337 1125
Size: 166 171 788
Fragments arranged by size:
788 171 166
Bsp1286I G_dGCh'C
Cuts at: 0 22 512 644 656 755 817 862 920
Size: 22 490 132 12 99 62 45 58
Cuts at: 920 1125
Size: 205
Fragments arranged by size:
490 205 132 99 62 58 45 22
12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 715 1125
Size: 715 410
BsrFI r'CCGG_y
Cuts at: 0 431 1125
Size: 431 694
BstAPI GCAn_nnn'nTGC
Cuts at: 0 745 1125
Size: 745 380
BstEII G'GTnAC_C
Cuts at: 0 384 1125
Size: 384 741
BstXI CCAn_nnnn'nTGG
Cuts at: 0 927 1125
Size: 927 198
DraIII CAC_nnn'GTG
Cuts at: 0 587 1125
Size: 587 538
EaeI y'GGCC_r
Cuts at: 0 249 1125
Size: 249 876
EarI CTCTTCn'nnn_
Cuts at: 0 850 1125
Size: 850 275
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 26 795 1125
Size: 26 769 330
Fragments arranged by size:
769 330 26
EcoICRI GAG'CTC
Cuts at: 0 510 654 815 1125
Size: 510 144 161 310
Fragments arranged by size:
510 310 161 144
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 109 241 463 471 867 994 1125
Size: 70 39 132 222 8 396 127 131
Fragments arranged by size:
396 222 132 131 127 70 39 8
HaeII r_GCGC'y
Cuts at: 0 1049 1125
Size: 1049 76
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 497 529 546 553 578 585 643 675
Size: 497 32 17 7 25 7 58 32
Cuts at: 675 1125
Size: 450
Fragments arranged by size:
497 450 58 32 32 25 17 7
7
HincII GTy'rAC
Cuts at: 0 203 1125
Size: 203 922
KpnI G_GTAC'C
Cuts at: 0 895 1125
Size: 895 230
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 539 645 1125
Size: 539 106 480
Fragments arranged by size:
539 480 106
MslI CAynn'nnrTG
Cuts at: 0 415 925 1125
Size: 415 510 200
Fragments arranged by size:
510 415 200
NdeI CA'TA_TG
Cuts at: 0 78 1125
Size: 78 1047
NheI G'CTAG_C
Cuts at: 0 865 1125
Size: 865 260
NsiI A_TGCA'T
Cuts at: 0 168 539 1125
Size: 168 371 586
Fragments arranged by size:
586 371 168
NspI r_CATG'y
Cuts at: 0 541 1125
Size: 541 584
PflMI CCAn_nnn'nTGG
Cuts at: 0 61 558 1125
Size: 61 497 567
Fragments arranged by size:
567 497 61
PspOMI G'GGCC_C
Cuts at: 0 18 1125
Size: 18 1107
PspXI vC'TCGA_Gb
Cuts at: 0 637 1125
Size: 637 488
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 430 462 1125
Size: 430 32 663
Fragments arranged by size:
663 430 32
SacI G_AGCT'C
Cuts at: 0 512 656 817 1125
Size: 512 144 161 308
Fragments arranged by size:
512 308 161 144
SapI GCTCTTCn'nnn_
Cuts at: 0 850 1125
Size: 850 275
SfcI C'TryA_G
Cuts at: 0 96 675 1125
Size: 96 579 450
Fragments arranged by size:
579 450 96
SmlI C'TyrA_G
Cuts at: 0 319 637 700 1125
Size: 319 318 63 425
Fragments arranged by size:
425 319 318 63
SphI G_CATG'C
Cuts at: 0 541 1125
Size: 541 584
SspI AAT'ATT
Cuts at: 0 104 1125
Size: 104 1021
StuI AGG'CCT
Cuts at: 0 1117 1125
Size: 1117 8
StyI C'CwwG_G
Cuts at: 0 329 1125
Size: 329 796
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 427 997 1125
Size: 427 570 128
Fragments arranged by size:
570 427 128
TatI w'GTAC_w
Cuts at: 0 308 1125
Size: 308 817
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 257 289 1125
Size: 257 32 836
Fragments arranged by size:
836 257 32
XhoI C'TCGA_G
Cuts at: 0 637 1125
Size: 637 488
Enzymes that do cut:
Acc65I AcuI AflII AflIII AleI AlwNI ApaI AvaI
BanI BanII BbvCI BciVI BfrBI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspMI BsrFI BstAPI BstEII BstXI
DraIII EaeI EarI EciI EcoICRI Eco57MI HaeII Hin4I
HincII KpnI MmeI MslI NdeI NheI NsiI NspI
PflMI PspOMI PspXI PsrI SacI SapI SfcI SmlI
SphI SspI StuI StyI TaqII TatI TstI XhoI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AgeI AhdI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbsI BcgI BclI BglI BglII BlpI
BplI BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI
BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI
BstBI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EagI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HindIII HpaI KasI MfeI
MluI MscI MspA1I NaeI NarI NcoI NgoMIV NotI
NruI PacI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SwaI Tth111I XbaI
XcmI XmaI XmnI ZraI