Restriction map for
pF1KB7625
(Linear) (Six-base) MAPSORT of: KIBB7625.seq Check: 501 from: 1 to: 948
KIBB7625.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 948
Size: 400 548
AflII C'TTAA_G
Cuts at: 0 195 948
Size: 195 753
AgeI A'CCGG_T
Cuts at: 0 473 948
Size: 473 475
AleI CACnn'nnGTG
Cuts at: 0 893 948
Size: 893 55
ApoI r'AATT_y
Cuts at: 0 374 523 556 943 948
Size: 374 149 33 387 5
Fragments arranged by size:
387 374 149 33 5
BamHI G'GATC_C
Cuts at: 0 39 259 948
Size: 39 220 689
Fragments arranged by size:
689 220 39
BanI G'GyrC_C
Cuts at: 0 852 948
Size: 852 96
BbsI GAAGACnn'nnnn_
Cuts at: 0 704 948
Size: 704 244
Bme1580I G_kGCm'C
Cuts at: 0 855 948
Size: 855 93
BsaWI w'CCGG_w
Cuts at: 0 42 473 948
Size: 42 431 475
Fragments arranged by size:
475 431 42
BseYI C'CCAG_C
Cuts at: 0 140 948
Size: 140 808
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 948
Size: 43 905
BsiEI CG_ry'CG
Cuts at: 0 47 948
Size: 47 901
BsmI GAATG_Cn'
Cuts at: 0 246 948
Size: 246 702
Bsp1286I G_dGCh'C
Cuts at: 0 855 948
Size: 855 93
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 470 948
Size: 470 478
BsrFI r'CCGG_y
Cuts at: 0 473 948
Size: 473 475
BsrGI T'GTAC_A
Cuts at: 0 806 948
Size: 806 142
BssSI C'ACGA_G
Cuts at: 0 584 948
Size: 584 364
BstXI CCAn_nnnn'nTGG
Cuts at: 0 843 895 948
Size: 843 52 53
Fragments arranged by size:
843 53 52
BstYI r'GATC_y
Cuts at: 0 39 189 259 948
Size: 39 150 70 689
Fragments arranged by size:
689 150 70 39
BtgI C'CryG_G
Cuts at: 0 692 836 948
Size: 692 144 112
Fragments arranged by size:
692 144 112
BtsI GCAGTG_nn'
Cuts at: 0 789 948
Size: 789 159
DraI TTT'AAA
Cuts at: 0 555 948
Size: 555 393
DraIII CAC_nnn'GTG
Cuts at: 0 859 948
Size: 859 89
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 948
Size: 19 929
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 948
Size: 400 548
EcoRV GAT'ATC
Cuts at: 0 333 948
Size: 333 615
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 502 534 948
Size: 502 32 414
Fragments arranged by size:
502 414 32
HindIII A'AGCT_T
Cuts at: 0 400 948
Size: 400 548
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 948
Size: 167 781
MslI CAynn'nnrTG
Cuts at: 0 893 948
Size: 893 55
MspA1I CmG'CkG
Cuts at: 0 147 948
Size: 147 801
NcoI C'CATG_G
Cuts at: 0 692 948
Size: 692 256
PstI C_TGCA'G
Cuts at: 0 467 948
Size: 467 481
SfcI C'TryA_G
Cuts at: 0 422 463 562 948
Size: 422 41 99 386
Fragments arranged by size:
422 386 99 41
SmlI C'TyrA_G
Cuts at: 0 195 948
Size: 195 753
SpeI A'CTAG_T
Cuts at: 0 279 948
Size: 279 669
StyI C'CwwG_G
Cuts at: 0 569 692 948
Size: 569 123 256
Fragments arranged by size:
569 256 123
TatI w'GTAC_w
Cuts at: 0 806 948
Size: 806 142
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 88 948
Size: 88 860
XmnI GAAnn'nnTTC
Cuts at: 0 621 948
Size: 621 327
Enzymes that do cut:
AcuI AflII AgeI AleI ApoI BamHI BanI BbsI
Bme1580I BsaWI BseYI BsgI BsiEI BsmI Bsp1286I BspMI
BsrFI BsrGI BssSI BstXI BstYI BtgI BtsI DraI
DraIII EciI Eco57MI EcoRV Hin4I HindIII MmeI MslI
MspA1I NcoI PstI SfcI SmlI SpeI StyI TatI
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AhdI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BanII BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaXI BseRI BsiHKAI BsiWI BsmBI BspEI BspHI BsrBI
BsrDI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI
ClaI CspCI DrdI EaeI EagI EarI EcoICRI EcoNI
EcoO109I EcoRI FalI FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MfeI MluI MscI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI
SspI StuI SwaI TaqII TstI Tth111I XbaI XhoI
XmaI ZraI