Restriction map for pF1KB7623
 (Linear) (Six-base) MAPSORT of: KIBB7623.seq  Check: 3766  from: 1  to: 942



KIBB7623.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

AccI GT'mk_AC

Cuts at:      0     818     942 
   Size:        818     124
 
AflIII A'CryG_T

Cuts at:      0     518     827     923     942 
   Size:        518     309      96      19

  Fragments arranged by size: 

                 518     309      96      19
 
AlwNI CAG_nnn'CTG

Cuts at:      0     456     942 
   Size:        456     486
 
ApaI G_GGCC'C

Cuts at:      0      86     200     691     907     942 
   Size:         86     114     491     216      35

  Fragments arranged by size: 

                 491     216     114      86      35
 
AvaI C'yCGr_G

Cuts at:      0     127     199     717     837     912     942 
   Size:        127      72     518     120      75      30

  Fragments arranged by size: 

                 518     127     120      75      72      30
 
BanI G'GyrC_C

Cuts at:      0     567     694     942 
   Size:        567     127     248

  Fragments arranged by size: 

                 567     248     127
 
BanII G_rGCy'C

Cuts at:      0      50      86     200     619     691     907     942 
   Size:         50      36     114     419      72     216      35

  Fragments arranged by size: 

                 419     216     114      72      50      36      35
 
BbeI G_GCGC'C

Cuts at:      0     571     698     942 
   Size:        571     127     244

  Fragments arranged by size: 

                 571     244     127
 
BglI GCCn_nnn'nGGC

Cuts at:      0      45     791     902     942 
   Size:         45     746     111      40

  Fragments arranged by size: 

                 746     111      45      40
 
Bme1580I G_kGCm'C

Cuts at:      0      86     200     691     907     942 
   Size:         86     114     491     216      35

  Fragments arranged by size: 

                 491     216     114      86      35
 
BmgBI CAC'GTC

Cuts at:      0     519     942 
   Size:        519     423
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     558     942 
   Size:        558     384
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     942 
   Size:         49     893
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     844     942 
   Size:        844      98
 
BsaAI yAC'GTr

Cuts at:      0     480     830     942 
   Size:        480     350     112

  Fragments arranged by size: 

                 480     350     112
 
BsaHI Gr'CG_yC

Cuts at:      0       5     568     695     942 
   Size:          5     563     127     247

  Fragments arranged by size: 

                 563     247     127       5
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     373     651     741     942 
   Size:        373     278      90     201

  Fragments arranged by size: 

                 373     278     201      90
 
BseYI C'CCAG_C

Cuts at:      0     246     649     942 
   Size:        246     403     293

  Fragments arranged by size: 

                 403     293     246
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     523     942 
   Size:        523     419
 
BsiEI CG_ry'CG

Cuts at:      0      55     942 
   Size:         55     887
 
BsiWI C'GTAC_G

Cuts at:      0     277     788     942 
   Size:        277     511     154

  Fragments arranged by size: 

                 511     277     154
 
Bsp1286I G_dGCh'C

Cuts at:      0      50      86     200     619     691     907     942 
   Size:         50      36     114     419      72     216      35

  Fragments arranged by size: 

                 419     216     114      72      50      36      35
 
BsrBI CCG'CTC

Cuts at:      0      33     126     579     942 
   Size:         33      93     453     363

  Fragments arranged by size: 

                 453     363      93      33
 
BsrFI r'CCGG_y

Cuts at:      0     529     745     942 
   Size:        529     216     197

  Fragments arranged by size: 

                 529     216     197
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     227     942 
   Size:        227     715
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     564     942 
   Size:        564     378
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     675     942 
   Size:        675     267
 
EaeI y'GGCC_r

Cuts at:      0      52     764     942 
   Size:         52     712     178

  Fragments arranged by size: 

                 712     178      52
 
EagI C'GGCC_G

Cuts at:      0      52     942 
   Size:         52     890
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     126     942 
   Size:        126     816
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     942 
   Size:         49     893
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     466     942 
   Size:        466     476
 
EcoO109I rG'GnC_Cy

Cuts at:      0      82     196     903     942 
   Size:         82     114     707      39

  Fragments arranged by size: 

                 707     114      82      39
 
HaeII r_GCGC'y

Cuts at:      0     571     577     698     782     942 
   Size:        571       6     121      84     160

  Fragments arranged by size: 

                 571     160     121      84       6
 
KasI G'GCGC_C

Cuts at:      0     567     694     942 
   Size:        567     127     248

  Fragments arranged by size: 

                 567     248     127
 
MluI A'CGCG_T

Cuts at:      0     923     942 
   Size:        923      19
 
MslI CAynn'nnrTG

Cuts at:      0     349     670     942 
   Size:        349     321     272

  Fragments arranged by size: 

                 349     321     272
 
MspA1I CmG'CkG

Cuts at:      0     267     456     494     621     942 
   Size:        267     189      38     127     321

  Fragments arranged by size: 

                 321     267     189     127      38
 
NaeI GCC'GGC

Cuts at:      0     531     942 
   Size:        531     411
 
NarI GG'CG_CC

Cuts at:      0     568     695     942 
   Size:        568     127     247

  Fragments arranged by size: 

                 568     247     127
 
NgoMIV G'CCGG_C

Cuts at:      0     529     942 
   Size:        529     413
 
NspI r_CATG'y

Cuts at:      0     711     942 
   Size:        711     231
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      78     456     942 
   Size:         78     378     486

  Fragments arranged by size: 

                 486     378      78
 
PmlI CAC'GTG

Cuts at:      0     830     942 
   Size:        830     112
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     304     336     942 
   Size:        304      32     606

  Fragments arranged by size: 

                 606     304      32
 
PspOMI G'GGCC_C

Cuts at:      0      82     196     687     903     942 
   Size:         82     114     491     216      39

  Fragments arranged by size: 

                 491     216     114      82      39
 
PspXI vC'TCGA_Gb

Cuts at:      0     837     942 
   Size:        837     105
 
PstI C_TGCA'G

Cuts at:      0     902     942 
   Size:        902      40
 
PvuII CAG'CTG

Cuts at:      0     456     942 
   Size:        456     486
 
SbfI CC_TGCA'GG

Cuts at:      0     902     942 
   Size:        902      40
 
SfcI C'TryA_G

Cuts at:      0     810     898     942 
   Size:        810      88      44

  Fragments arranged by size: 

                 810      88      44
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     902     942 
   Size:        902      40
 
SfoI GGC'GCC

Cuts at:      0     569     696     942 
   Size:        569     127     246

  Fragments arranged by size: 

                 569     246     127
 
SmlI C'TyrA_G

Cuts at:      0     127     837     859     942 
   Size:        127     710      22      83

  Fragments arranged by size: 

                 710     127      83      22
 
SphI G_CATG'C

Cuts at:      0     711     942 
   Size:        711     231
 
StyI C'CwwG_G

Cuts at:      0     191     942 
   Size:        191     751
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     663     942 
   Size:        663     279
 
TatI w'GTAC_w

Cuts at:      0     932     942 
   Size:        932      10
 
XhoI C'TCGA_G

Cuts at:      0     127     837     942 
   Size:        127     710     105

  Fragments arranged by size: 

                 710     127     105
 

 Enzymes that do cut:

     AccI   AflIII    AlwNI     ApaI     AvaI     BanI    BanII     BbeI
     BglI Bme1580I    BmgBI     BmrI     BpmI    BpuEI    BsaAI    BsaHI
    BseRI    BseYI     BsgI    BsiEI    BsiWI Bsp1286I    BsrBI    BsrFI
   BstAPI    BstXI     DrdI     EaeI     EagI     EciI  Eco57MI    EcoNI
 EcoO109I    HaeII     KasI     MluI     MslI   MspA1I     NaeI     NarI
   NgoMIV     NspI    PflMI     PmlI     PpiI   PspOMI    PspXI     PstI
    PvuII     SbfI     SfcI     SfiI     SfoI     SmlI     SphI     StyI
    TaqII     TatI     XhoI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI    BglII     BlpI     BmtI     BplI   Bpu10I     BsaI
    BsaBI    BsaWI    BsaXI  BsiHKAI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrDI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     EarI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MmeI
     MscI     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI     PfoI     PmeI    PpuMI    PshAI     PsiI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     ScaI
    SexAI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI
     StuI     SwaI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI
     ZraI
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