(Linear) (Six-base) MAPSORT of: KIBB7623.seq Check: 3766 from: 1 to: 942 KIBB7623.orf With 182 enzymes: SgfI * March 3, 2008 11:02 .. AccI GT'mk_AC Cuts at: 0 818 942 Size: 818 124 AflIII A'CryG_T Cuts at: 0 518 827 923 942 Size: 518 309 96 19 Fragments arranged by size: 518 309 96 19 AlwNI CAG_nnn'CTG Cuts at: 0 456 942 Size: 456 486 ApaI G_GGCC'C Cuts at: 0 86 200 691 907 942 Size: 86 114 491 216 35 Fragments arranged by size: 491 216 114 86 35 AvaI C'yCGr_G Cuts at: 0 127 199 717 837 912 942 Size: 127 72 518 120 75 30 Fragments arranged by size: 518 127 120 75 72 30 BanI G'GyrC_C Cuts at: 0 567 694 942 Size: 567 127 248 Fragments arranged by size: 567 248 127 BanII G_rGCy'C Cuts at: 0 50 86 200 619 691 907 942 Size: 50 36 114 419 72 216 35 Fragments arranged by size: 419 216 114 72 50 36 35 BbeI G_GCGC'C Cuts at: 0 571 698 942 Size: 571 127 244 Fragments arranged by size: 571 244 127 BglI GCCn_nnn'nGGC Cuts at: 0 45 791 902 942 Size: 45 746 111 40 Fragments arranged by size: 746 111 45 40 Bme1580I G_kGCm'C Cuts at: 0 86 200 691 907 942 Size: 86 114 491 216 35 Fragments arranged by size: 491 216 114 86 35 BmgBI CAC'GTC Cuts at: 0 519 942 Size: 519 423 BmrI ACTGGGnnnn_n' Cuts at: 0 558 942 Size: 558 384 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 49 942 Size: 49 893 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 844 942 Size: 844 98 BsaAI yAC'GTr Cuts at: 0 480 830 942 Size: 480 350 112 Fragments arranged by size: 480 350 112 BsaHI Gr'CG_yC Cuts at: 0 5 568 695 942 Size: 5 563 127 247 Fragments arranged by size: 563 247 127 5 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 373 651 741 942 Size: 373 278 90 201 Fragments arranged by size: 373 278 201 90 BseYI C'CCAG_C Cuts at: 0 246 649 942 Size: 246 403 293 Fragments arranged by size: 403 293 246 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 523 942 Size: 523 419 BsiEI CG_ry'CG Cuts at: 0 55 942 Size: 55 887 BsiWI C'GTAC_G Cuts at: 0 277 788 942 Size: 277 511 154 Fragments arranged by size: 511 277 154 Bsp1286I G_dGCh'C Cuts at: 0 50 86 200 619 691 907 942 Size: 50 36 114 419 72 216 35 Fragments arranged by size: 419 216 114 72 50 36 35 BsrBI CCG'CTC Cuts at: 0 33 126 579 942 Size: 33 93 453 363 Fragments arranged by size: 453 363 93 33 BsrFI r'CCGG_y Cuts at: 0 529 745 942 Size: 529 216 197 Fragments arranged by size: 529 216 197 BstAPI GCAn_nnn'nTGC Cuts at: 0 227 942 Size: 227 715 BstXI CCAn_nnnn'nTGG Cuts at: 0 564 942 Size: 564 378 DrdI GACnn_nn'nnGTC Cuts at: 0 675 942 Size: 675 267 EaeI y'GGCC_r Cuts at: 0 52 764 942 Size: 52 712 178 Fragments arranged by size: 712 178 52 EagI C'GGCC_G Cuts at: 0 52 942 Size: 52 890 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 126 942 Size: 126 816 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 49 942 Size: 49 893 EcoNI CCTnn'n_nnAGG Cuts at: 0 466 942 Size: 466 476 EcoO109I rG'GnC_Cy Cuts at: 0 82 196 903 942 Size: 82 114 707 39 Fragments arranged by size: 707 114 82 39 HaeII r_GCGC'y Cuts at: 0 571 577 698 782 942 Size: 571 6 121 84 160 Fragments arranged by size: 571 160 121 84 6 KasI G'GCGC_C Cuts at: 0 567 694 942 Size: 567 127 248 Fragments arranged by size: 567 248 127 MluI A'CGCG_T Cuts at: 0 923 942 Size: 923 19 MslI CAynn'nnrTG Cuts at: 0 349 670 942 Size: 349 321 272 Fragments arranged by size: 349 321 272 MspA1I CmG'CkG Cuts at: 0 267 456 494 621 942 Size: 267 189 38 127 321 Fragments arranged by size: 321 267 189 127 38 NaeI GCC'GGC Cuts at: 0 531 942 Size: 531 411 NarI GG'CG_CC Cuts at: 0 568 695 942 Size: 568 127 247 Fragments arranged by size: 568 247 127 NgoMIV G'CCGG_C Cuts at: 0 529 942 Size: 529 413 NspI r_CATG'y Cuts at: 0 711 942 Size: 711 231 PflMI CCAn_nnn'nTGG Cuts at: 0 78 456 942 Size: 78 378 486 Fragments arranged by size: 486 378 78 PmlI CAC'GTG Cuts at: 0 830 942 Size: 830 112 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 304 336 942 Size: 304 32 606 Fragments arranged by size: 606 304 32 PspOMI G'GGCC_C Cuts at: 0 82 196 687 903 942 Size: 82 114 491 216 39 Fragments arranged by size: 491 216 114 82 39 PspXI vC'TCGA_Gb Cuts at: 0 837 942 Size: 837 105 PstI C_TGCA'G Cuts at: 0 902 942 Size: 902 40 PvuII CAG'CTG Cuts at: 0 456 942 Size: 456 486 SbfI CC_TGCA'GG Cuts at: 0 902 942 Size: 902 40 SfcI C'TryA_G Cuts at: 0 810 898 942 Size: 810 88 44 Fragments arranged by size: 810 88 44 SfiI GGCCn_nnn'nGGCC Cuts at: 0 902 942 Size: 902 40 SfoI GGC'GCC Cuts at: 0 569 696 942 Size: 569 127 246 Fragments arranged by size: 569 246 127 SmlI C'TyrA_G Cuts at: 0 127 837 859 942 Size: 127 710 22 83 Fragments arranged by size: 710 127 83 22 SphI G_CATG'C Cuts at: 0 711 942 Size: 711 231 StyI C'CwwG_G Cuts at: 0 191 942 Size: 191 751 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 663 942 Size: 663 279 TatI w'GTAC_w Cuts at: 0 932 942 Size: 932 10 XhoI C'TCGA_G Cuts at: 0 127 837 942 Size: 127 710 105 Fragments arranged by size: 710 127 105 Enzymes that do cut: AccI AflIII AlwNI ApaI AvaI BanI BanII BbeI BglI Bme1580I BmgBI BmrI BpmI BpuEI BsaAI BsaHI BseRI BseYI BsgI BsiEI BsiWI Bsp1286I BsrBI BsrFI BstAPI BstXI DrdI EaeI EagI EciI Eco57MI EcoNI EcoO109I HaeII KasI MluI MslI MspA1I NaeI NarI NgoMIV NspI PflMI PmlI PpiI PspOMI PspXI PstI PvuII SbfI SfcI SfiI SfoI SmlI SphI StyI TaqII TatI XhoI Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmtI BplI Bpu10I BsaI BsaBI BsaWI BsaXI BsiHKAI BsmI BsmBI BspEI BspHI BspMI BsrDI BsrGI BssHII BssSI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII EarI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MmeI MscI NcoI NdeI NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PpuMI PshAI PsiI PsrI PvuI RsrII SacI SacII SalI SanDI SapI ScaI SexAI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI TstI Tth111I XbaI XcmI XmaI XmnI ZraI