Restriction map for
pF1KB7622
(Linear) (Six-base) MAPSORT of: KIBB7622.seq Check: 8124 from: 1 to: 1104
KIBB7622.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AccI GT'mk_AC
Cuts at: 0 432 1104
Size: 432 672
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 605 762 1104
Size: 605 157 342
Fragments arranged by size:
605 342 157
AgeI A'CCGG_T
Cuts at: 0 417 1104
Size: 417 687
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 317 1104
Size: 317 787
ApaI G_GGCC'C
Cuts at: 0 197 1104
Size: 197 907
ApoI r'AATT_y
Cuts at: 0 65 674 812 954 1104
Size: 65 609 138 142 150
Fragments arranged by size:
609 150 142 138 65
AseI AT'TA_AT
Cuts at: 0 968 1104
Size: 968 136
BamHI G'GATC_C
Cuts at: 0 90 1104
Size: 90 1014
BanII G_rGCy'C
Cuts at: 0 197 548 790 1104
Size: 197 351 242 314
Fragments arranged by size:
351 314 242 197
BbsI GAAGACnn'nnnn_
Cuts at: 0 464 1071 1104
Size: 464 607 33
Fragments arranged by size:
607 464 33
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 209 243 1104
Size: 209 34 861
Fragments arranged by size:
861 209 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 127 169 1104
Size: 127 42 935
Fragments arranged by size:
935 127 42
BlpI GC'TnA_GC
Cuts at: 0 789 1104
Size: 789 315
Bme1580I G_kGCm'C
Cuts at: 0 197 1104
Size: 197 907
BmrI ACTGGGnnnn_n'
Cuts at: 0 998 1104
Size: 998 106
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 310 400 926 1104
Size: 32 278 90 526 178
Fragments arranged by size:
526 278 178 90 32
Bpu10I CC'TnA_GC
Cuts at: 0 241 482 1104
Size: 241 241 622
Fragments arranged by size:
622 241 241
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 455 511 1104
Size: 455 56 593
Fragments arranged by size:
593 455 56
BsaBI GATnn'nnATC
Cuts at: 0 344 1104
Size: 344 760
BsaWI w'CCGG_w
Cuts at: 0 417 1104
Size: 417 687
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 100 376 1104
Size: 100 276 728
Fragments arranged by size:
728 276 100
BsiHKAI G_wGCw'C
Cuts at: 0 548 597 1104
Size: 548 49 507
Fragments arranged by size:
548 507 49
BsmI GAATG_Cn'
Cuts at: 0 859 1012 1104
Size: 859 153 92
Fragments arranged by size:
859 153 92
BsmBI CGTCTCn'nnnn_
Cuts at: 0 244 1104
Size: 244 860
Bsp1286I G_dGCh'C
Cuts at: 0 197 548 597 790 1104
Size: 197 351 49 193 314
Fragments arranged by size:
351 314 197 193 49
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 701 1104
Size: 701 403
BsrFI r'CCGG_y
Cuts at: 0 417 1104
Size: 417 687
BstYI r'GATC_y
Cuts at: 0 90 1104
Size: 90 1014
Bsu36I CC'TnA_GG
Cuts at: 0 556 1104
Size: 556 548
BtsI GCAGTG_nn'
Cuts at: 0 374 1104
Size: 374 730
DraI TTT'AAA
Cuts at: 0 681 1104
Size: 681 423
EarI CTCTTCn'nnn_
Cuts at: 0 50 98 1104
Size: 50 48 1006
Fragments arranged by size:
1006 50 48
EcoICRI GAG'CTC
Cuts at: 0 546 1104
Size: 546 558
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 310 400 605 762 926 1104
Size: 32 278 90 205 157 164 178
Fragments arranged by size:
278 205 178 164 157 90 32
EcoO109I rG'GnC_Cy
Cuts at: 0 194 1104
Size: 194 910
EcoRI G'AATT_C
Cuts at: 0 812 954 1104
Size: 812 142 150
Fragments arranged by size:
812 150 142
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 101 133 451 483 1104
Size: 101 32 318 32 621
Fragments arranged by size:
621 318 101 32 32
HincII GTy'rAC
Cuts at: 0 433 1104
Size: 433 671
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 62 177 1104
Size: 62 115 927
Fragments arranged by size:
927 115 62
MslI CAynn'nnrTG
Cuts at: 0 566 755 880 1104
Size: 566 189 125 224
Fragments arranged by size:
566 224 189 125
PflMI CCAn_nnn'nTGG
Cuts at: 0 355 1104
Size: 355 749
PspOMI G'GGCC_C
Cuts at: 0 193 1104
Size: 193 911
SacI G_AGCT'C
Cuts at: 0 548 1104
Size: 548 556
SalI G'TCGA_C
Cuts at: 0 431 1104
Size: 431 673
ScaI AGT'ACT
Cuts at: 0 449 1104
Size: 449 655
SfcI C'TryA_G
Cuts at: 0 322 1104
Size: 322 782
SmlI C'TyrA_G
Cuts at: 0 470 526 1104
Size: 470 56 578
Fragments arranged by size:
578 470 56
SpeI A'CTAG_T
Cuts at: 0 450 1104
Size: 450 654
StyI C'CwwG_G
Cuts at: 0 704 1104
Size: 704 400
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 652 1104
Size: 652 452
TatI w'GTAC_w
Cuts at: 0 447 1104
Size: 447 657
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 187 643 1104
Size: 187 456 461
Fragments arranged by size:
461 456 187
XmnI GAAnn'nnTTC
Cuts at: 0 65 859 1010 1082 1104
Size: 65 794 151 72 22
Fragments arranged by size:
794 151 72 65 22
Enzymes that do cut:
AccI AcuI AgeI AlfI ApaI ApoI AseI BamHI
BanII BbsI BcgI BciVI BlpI Bme1580I BmrI BpmI
Bpu10I BpuEI BsaBI BsaWI BseRI BsiHKAI BsmI BsmBI
Bsp1286I BspMI BsrFI BstYI Bsu36I BtsI DraI EarI
EcoICRI Eco57MI EcoO109I EcoRI Hin4I HincII MmeI MslI
PflMI PspOMI SacI SalI ScaI SfcI SmlI SpeI
StyI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AhdI
AleI AloI AlwNI ApaLI AscI AsiSI AvaI AvrII
BaeI BanI BbeI BbvCI BclI BfrBI BglI BglII
BmgBI BmtI BplI BsaI BsaAI BsaHI BsaXI BseYI
BsgI BsiEI BsiWI BspEI BspHI BsrBI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgI
BtgZI ClaI CspCI DraIII DrdI EaeI EagI EciI
EcoNI EcoRV FalI FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MfeI MluI MscI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII
RsrII SacII SanDI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SphI SrfI SspI StuI
SwaI TstI Tth111I XbaI XhoI XmaI ZraI