Restriction map for
pF1KB7607
(Linear) (Six-base) MAPSORT of: KIBB7607.seq Check: 969 from: 1 to: 897
KIBB7607.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AatII G_ACGT'C
Cuts at: 0 197 897
Size: 197 700
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 897
Size: 241 656
AleI CACnn'nnGTG
Cuts at: 0 388 466 897
Size: 388 78 431
Fragments arranged by size:
431 388 78
ApaI G_GGCC'C
Cuts at: 0 26 694 897
Size: 26 668 203
Fragments arranged by size:
668 203 26
ApoI r'AATT_y
Cuts at: 0 802 829 897
Size: 802 27 68
Fragments arranged by size:
802 68 27
AvaI C'yCGr_G
Cuts at: 0 18 897
Size: 18 879
BanI G'GyrC_C
Cuts at: 0 675 775 820 897
Size: 675 100 45 77
Fragments arranged by size:
675 100 77 45
BanII G_rGCy'C
Cuts at: 0 26 493 637 694 897
Size: 26 467 144 57 203
Fragments arranged by size:
467 203 144 57 26
BbeI G_GCGC'C
Cuts at: 0 779 897
Size: 779 118
BglI GCCn_nnn'nGGC
Cuts at: 0 449 897
Size: 449 448
BglII A'GATC_T
Cuts at: 0 890 897
Size: 890 7
Bme1580I G_kGCm'C
Cuts at: 0 26 92 367 680 694 823 897
Size: 26 66 275 313 14 129 74
Fragments arranged by size:
313 275 129 74 66 26 14
BmgBI CAC'GTC
Cuts at: 0 368 897
Size: 368 529
BmrI ACTGGGnnnn_n'
Cuts at: 0 56 177 735 897
Size: 56 121 558 162
Fragments arranged by size:
558 162 121 56
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 181 507 897
Size: 172 9 326 390
Fragments arranged by size:
390 326 172 9
BsaI GGTCTCn'nnnn_
Cuts at: 0 632 897
Size: 632 265
BsaHI Gr'CG_yC
Cuts at: 0 194 776 897
Size: 194 582 121
Fragments arranged by size:
582 194 121
BsaWI w'CCGG_w
Cuts at: 0 266 897
Size: 266 631
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 154 184 897
Size: 154 30 713
Fragments arranged by size:
713 154 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 169 223 897
Size: 169 54 674
Fragments arranged by size:
674 169 54
BseYI C'CCAG_C
Cuts at: 0 492 529 680 897
Size: 492 37 151 217
Fragments arranged by size:
492 217 151 37
BsiHKAI G_wGCw'C
Cuts at: 0 637 897
Size: 637 260
BsmBI CGTCTCn'nnnn_
Cuts at: 0 557 897
Size: 557 340
Bsp1286I G_dGCh'C
Cuts at: 0 26 92 367 493 637 680 694 823
Size: 26 66 275 126 144 43 14 129
Cuts at: 823 897
Size: 74
Fragments arranged by size:
275 144 129 126 74 66 43 26
14
BstAPI GCAn_nnn'nTGC
Cuts at: 0 172 897
Size: 172 725
BstXI CCAn_nnnn'nTGG
Cuts at: 0 468 772 897
Size: 468 304 125
Fragments arranged by size:
468 304 125
BstYI r'GATC_y
Cuts at: 0 69 890 897
Size: 69 821 7
Fragments arranged by size:
821 69 7
Bsu36I CC'TnA_GG
Cuts at: 0 447 515 543 897
Size: 447 68 28 354
Fragments arranged by size:
447 354 68 28
BtgI C'CryG_G
Cuts at: 0 461 671 722 788 815 845 897
Size: 461 210 51 66 27 30 52
Fragments arranged by size:
461 210 66 52 51 30 27
BtsI GCAGTG_nn'
Cuts at: 0 473 897
Size: 473 424
EaeI y'GGCC_r
Cuts at: 0 470 897
Size: 470 427
EarI CTCTTCn'nnn_
Cuts at: 0 612 897
Size: 612 285
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 552 606 897
Size: 552 54 291
Fragments arranged by size:
552 291 54
EcoICRI GAG'CTC
Cuts at: 0 635 897
Size: 635 262
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 181 241 507 897
Size: 172 9 60 266 390
Fragments arranged by size:
390 266 172 60 9
EcoO109I rG'GnC_Cy
Cuts at: 0 22 23 690 727 810 897
Size: 22 1 667 37 83 87
Fragments arranged by size:
667 87 83 37 22 1
EcoRI G'AATT_C
Cuts at: 0 802 897
Size: 802 95
HaeII r_GCGC'y
Cuts at: 0 104 779 897
Size: 104 675 118
Fragments arranged by size:
675 118 104
HincII GTy'rAC
Cuts at: 0 39 897
Size: 39 858
KasI G'GCGC_C
Cuts at: 0 775 897
Size: 775 122
MscI TGG'CCA
Cuts at: 0 472 897
Size: 472 425
MslI CAynn'nnrTG
Cuts at: 0 388 466 897
Size: 388 78 431
Fragments arranged by size:
431 388 78
MspA1I CmG'CkG
Cuts at: 0 117 525 647 897
Size: 117 408 122 250
Fragments arranged by size:
408 250 122 117
NarI GG'CG_CC
Cuts at: 0 776 897
Size: 776 121
NcoI C'CATG_G
Cuts at: 0 671 845 897
Size: 671 174 52
Fragments arranged by size:
671 174 52
PasI CC'CwG_GG
Cuts at: 0 322 897
Size: 322 575
PpuMI rG'GwC_Cy
Cuts at: 0 727 810 897
Size: 727 83 87
Fragments arranged by size:
727 87 83
PspOMI G'GGCC_C
Cuts at: 0 22 690 897
Size: 22 668 207
Fragments arranged by size:
668 207 22
SacI G_AGCT'C
Cuts at: 0 637 897
Size: 637 260
SapI GCTCTTCn'nnn_
Cuts at: 0 612 897
Size: 612 285
SfcI C'TryA_G
Cuts at: 0 423 780 897
Size: 423 357 117
Fragments arranged by size:
423 357 117
SfoI GGC'GCC
Cuts at: 0 777 897
Size: 777 120
SmaI CCC'GGG
Cuts at: 0 20 897
Size: 20 877
StyI C'CwwG_G
Cuts at: 0 44 335 671 845 897
Size: 44 291 336 174 52
Fragments arranged by size:
336 291 174 52 44
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 825 857 897
Size: 825 32 40
Fragments arranged by size:
825 40 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 852 897
Size: 852 45
XmaI C'CCGG_G
Cuts at: 0 18 897
Size: 18 879
ZraI GAC'GTC
Cuts at: 0 195 897
Size: 195 702
Enzymes that do cut:
AatII AcuI AleI ApaI ApoI AvaI BanI BanII
BbeI BglI BglII Bme1580I BmgBI BmrI BpmI BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI BsmBI Bsp1286I
BstAPI BstXI BstYI Bsu36I BtgI BtsI EaeI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI HaeII HincII KasI
MscI MslI MspA1I NarI NcoI PasI PpuMI PspOMI
SacI SapI SfcI SfoI SmaI StyI TstI XcmI
XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI
BfrBI BlpI BmtI BplI Bpu10I BpuEI BsaAI BsaBI
BsgI BsiEI BsiWI BsmI BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EcoNI
EcoRV FalI FseI FspI FspAI Hin4I HindIII HpaI
KpnI MfeI MluI MmeI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI
PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI
SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TatI Tth111I XbaI
XhoI XmnI