Restriction map for
pF1KB7595
(Linear) (Six-base) MAPSORT of: KIBB7595.seq Check: 1281 from: 1 to: 699
KIBB7595.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AccI GT'mk_AC
Cuts at: 0 677 699
Size: 677 22
AfeI AGC'GCT
Cuts at: 0 627 699
Size: 627 72
AleI CACnn'nnGTG
Cuts at: 0 445 699
Size: 445 254
AlwNI CAG_nnn'CTG
Cuts at: 0 312 699
Size: 312 387
ApaI G_GGCC'C
Cuts at: 0 71 699
Size: 71 628
AvaI C'yCGr_G
Cuts at: 0 249 699
Size: 249 450
BanI G'GyrC_C
Cuts at: 0 127 375 699
Size: 127 248 324
Fragments arranged by size:
324 248 127
BanII G_rGCy'C
Cuts at: 0 71 79 526 578 699
Size: 71 8 447 52 121
Fragments arranged by size:
447 121 71 52 8
BbeI G_GCGC'C
Cuts at: 0 131 699
Size: 131 568
BbsI GAAGACnn'nnnn_
Cuts at: 0 64 588 699
Size: 64 524 111
Fragments arranged by size:
524 111 64
BbvCI CC'TCA_GC
Cuts at: 0 97 293 554 699
Size: 97 196 261 145
Fragments arranged by size:
261 196 145 97
BglI GCCn_nnn'nGGC
Cuts at: 0 43 337 699
Size: 43 294 362
Fragments arranged by size:
362 294 43
Bme1580I G_kGCm'C
Cuts at: 0 71 699
Size: 71 628
BmgBI CAC'GTC
Cuts at: 0 27 699
Size: 27 672
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 289 625 699
Size: 63 226 336 74
Fragments arranged by size:
336 226 74 63
Bpu10I CC'TnA_GC
Cuts at: 0 97 293 554 699
Size: 97 196 261 145
Fragments arranged by size:
261 196 145 97
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 272 699
Size: 272 427
BsaI GGTCTCn'nnnn_
Cuts at: 0 532 699
Size: 532 167
BsaHI Gr'CG_yC
Cuts at: 0 128 699
Size: 128 571
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 55 442 496 699
Size: 55 387 54 203
Fragments arranged by size:
387 203 55 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 699
Size: 136 563
BsiHKAI G_wGCw'C
Cuts at: 0 7 452 699
Size: 7 445 247
Fragments arranged by size:
445 247 7
BsmBI CGTCTCn'nnnn_
Cuts at: 0 486 699
Size: 486 213
Bsp1286I G_dGCh'C
Cuts at: 0 7 71 79 452 526 578 699
Size: 7 64 8 373 74 52 121
Fragments arranged by size:
373 121 74 64 52 8 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 663 699
Size: 663 36
BsrDI GCAATG_nn'
Cuts at: 0 689 699
Size: 689 10
BstEII G'GTnAC_C
Cuts at: 0 613 699
Size: 613 86
BstXI CCAn_nnnn'nTGG
Cuts at: 0 348 585 699
Size: 348 237 114
Fragments arranged by size:
348 237 114
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 459 699
Size: 459 240
EaeI y'GGCC_r
Cuts at: 0 35 338 699
Size: 35 303 361
Fragments arranged by size:
361 303 35
EarI CTCTTCn'nnn_
Cuts at: 0 109 145 176 421 699
Size: 109 36 31 245 278
Fragments arranged by size:
278 245 109 36 31
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 289 625 699
Size: 63 226 336 74
Fragments arranged by size:
336 226 74 63
EcoNI CCTnn'n_nnAGG
Cuts at: 0 418 531 699
Size: 418 113 168
Fragments arranged by size:
418 168 113
EcoO109I rG'GnC_Cy
Cuts at: 0 67 68 195 387 411 699
Size: 67 1 127 192 24 288
Fragments arranged by size:
288 192 127 67 24 1
HaeII r_GCGC'y
Cuts at: 0 49 131 629 699
Size: 49 82 498 70
Fragments arranged by size:
498 82 70 49
HincII GTy'rAC
Cuts at: 0 501 699
Size: 501 198
KasI G'GCGC_C
Cuts at: 0 127 699
Size: 127 572
MscI TGG'CCA
Cuts at: 0 340 699
Size: 340 359
MslI CAynn'nnrTG
Cuts at: 0 445 699
Size: 445 254
MspA1I CmG'CkG
Cuts at: 0 369 603 699
Size: 369 234 96
Fragments arranged by size:
369 234 96
NarI GG'CG_CC
Cuts at: 0 128 699
Size: 128 571
PasI CC'CwG_GG
Cuts at: 0 72 191 699
Size: 72 119 508
Fragments arranged by size:
508 119 72
PflMI CCAn_nnn'nTGG
Cuts at: 0 372 566 699
Size: 372 194 133
Fragments arranged by size:
372 194 133
PfoI T'CCnGG_A
Cuts at: 0 20 699
Size: 20 679
PpuMI rG'GwC_Cy
Cuts at: 0 195 411 699
Size: 195 216 288
Fragments arranged by size:
288 216 195
PshAI GACnn'nnGTC
Cuts at: 0 496 699
Size: 496 203
PspOMI G'GGCC_C
Cuts at: 0 67 699
Size: 67 632
PvuII CAG'CTG
Cuts at: 0 369 603 699
Size: 369 234 96
Fragments arranged by size:
369 234 96
SapI GCTCTTCn'nnn_
Cuts at: 0 145 699
Size: 145 554
SfcI C'TryA_G
Cuts at: 0 263 699
Size: 263 436
SfoI GGC'GCC
Cuts at: 0 129 699
Size: 129 570
SmlI C'TyrA_G
Cuts at: 0 287 699
Size: 287 412
StyI C'CwwG_G
Cuts at: 0 426 494 699
Size: 426 68 205
Fragments arranged by size:
426 205 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 457 472 632 699
Size: 457 15 160 67
Fragments arranged by size:
457 160 67 15
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 333 699
Size: 333 366
Enzymes that do cut:
AccI AfeI AleI AlwNI ApaI AvaI BanI BanII
BbeI BbsI BbvCI BglI Bme1580I BmgBI BpmI Bpu10I
BpuEI BsaI BsaHI BseRI BsgI BsiHKAI BsmBI Bsp1286I
BspMI BsrDI BstEII BstXI BtgZI EaeI EarI Eco57MI
EcoNI EcoO109I HaeII HincII KasI MscI MslI MspA1I
NarI PasI PflMI PfoI PpuMI PshAI PspOMI PvuII
SapI SfcI SfoI SmlI StyI TaqII XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AflII AflIII AgeI
AhdI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BcgI BciVI BclI BfrBI BglII
BlpI BmrI BmtI BplI BsaAI BsaBI BsaWI BsaXI
BseYI BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstYI BstZ17I Bsu36I
BtgI BtsI ClaI CspCI DraI DraIII DrdI EagI
EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HindIII HpaI KpnI MfeI MluI MmeI NaeI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PmeI PmlI PpiI PsiI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI