Restriction map for
pF1KB7559
(Linear) (Six-base) MAPSORT of: KIBB7559.seq Check: 1641 from: 1 to: 618
KIBB7559.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 238 482 618
Size: 238 244 136
Fragments arranged by size:
244 238 136
AfeI AGC'GCT
Cuts at: 0 172 618
Size: 172 446
AhdI GACnn_n'nnGTC
Cuts at: 0 132 618
Size: 132 486
ApaLI G'TGCA_C
Cuts at: 0 517 618
Size: 517 101
AvaI C'yCGr_G
Cuts at: 0 552 618
Size: 552 66
BanI G'GyrC_C
Cuts at: 0 408 618
Size: 408 210
BanII G_rGCy'C
Cuts at: 0 13 298 618
Size: 13 285 320
Fragments arranged by size:
320 285 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 564 618
Size: 564 54
BlpI GC'TnA_GC
Cuts at: 0 297 367 618
Size: 297 70 251
Fragments arranged by size:
297 251 70
Bme1580I G_kGCm'C
Cuts at: 0 187 354 413 521 618
Size: 187 167 59 108 97
Fragments arranged by size:
187 167 108 97 59
BmrI ACTGGGnnnn_n'
Cuts at: 0 116 517 618
Size: 116 401 101
Fragments arranged by size:
401 116 101
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 366 618
Size: 366 252
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 618
Size: 373 245
BsaI GGTCTCn'nnnn_
Cuts at: 0 555 618
Size: 555 63
BsaAI yAC'GTr
Cuts at: 0 516 618
Size: 516 102
BsaWI w'CCGG_w
Cuts at: 0 24 561 618
Size: 24 537 57
Fragments arranged by size:
537 57 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 43 157 160 178 181 618
Size: 43 114 3 18 3 437
Fragments arranged by size:
437 114 43 18 3 3
BseYI C'CCAG_C
Cuts at: 0 232 618
Size: 232 386
BsiHKAI G_wGCw'C
Cuts at: 0 521 618
Size: 521 97
Bsp1286I G_dGCh'C
Cuts at: 0 13 187 298 354 413 521 618
Size: 13 174 111 56 59 108 97
Fragments arranged by size:
174 111 108 97 59 56 13
BspEI T'CCGG_A
Cuts at: 0 24 618
Size: 24 594
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 238 482 618
Size: 238 244 136
Fragments arranged by size:
244 238 136
BsrFI r'CCGG_y
Cuts at: 0 190 618
Size: 190 428
BstAPI GCAn_nnn'nTGC
Cuts at: 0 486 618
Size: 486 132
BtgI C'CryG_G
Cuts at: 0 5 93 618
Size: 5 88 525
Fragments arranged by size:
525 88 5
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 450 618
Size: 450 168
DraIII CAC_nnn'GTG
Cuts at: 0 525 618
Size: 525 93
EaeI y'GGCC_r
Cuts at: 0 287 332 538 618
Size: 287 45 206 80
Fragments arranged by size:
287 206 80 45
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 366 618
Size: 366 252
EcoNI CCTnn'n_nnAGG
Cuts at: 0 78 381 618
Size: 78 303 237
Fragments arranged by size:
303 237 78
EcoO109I rG'GnC_Cy
Cuts at: 0 150 618
Size: 150 468
HaeII r_GCGC'y
Cuts at: 0 174 618
Size: 174 444
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 118 150 618
Size: 118 32 468
Fragments arranged by size:
468 118 32
MscI TGG'CCA
Cuts at: 0 540 618
Size: 540 78
MspA1I CmG'CkG
Cuts at: 0 54 95 324 483 618
Size: 54 41 229 159 135
Fragments arranged by size:
229 159 135 54 41
NaeI GCC'GGC
Cuts at: 0 192 618
Size: 192 426
NgoMIV G'CCGG_C
Cuts at: 0 190 618
Size: 190 428
PasI CC'CwG_GG
Cuts at: 0 404 618
Size: 404 214
PpuMI rG'GwC_Cy
Cuts at: 0 150 618
Size: 150 468
PstI C_TGCA'G
Cuts at: 0 53 618
Size: 53 565
PvuII CAG'CTG
Cuts at: 0 483 618
Size: 483 135
SacII CC_GC'GG
Cuts at: 0 96 618
Size: 96 522
SfcI C'TryA_G
Cuts at: 0 49 618
Size: 49 569
SmlI C'TyrA_G
Cuts at: 0 388 618
Size: 388 230
StyI C'CwwG_G
Cuts at: 0 290 618
Size: 290 328
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 396 536 618
Size: 396 140 82
Fragments arranged by size:
396 140 82
Enzymes that do cut:
AarI AfeI AhdI ApaLI AvaI BanI BanII BbsI
BlpI Bme1580I BmrI BpmI BpuEI BsaI BsaAI BsaWI
BseRI BseYI BsiHKAI Bsp1286I BspEI BspMI BsrFI BstAPI
BtgI BtgZI DraIII EaeI Eco57MI EcoNI EcoO109I HaeII
Hin4I MscI MspA1I NaeI NgoMIV PasI PpuMI PstI
PvuII SacII SfcI SmlI StyI TaqII
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BmgBI BmtI BplI Bpu10I
BsaBI BsaHI BsaXI BsgI BsiEI BsiWI BsmI BsmBI
BspHI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtsI ClaI CspCI DraI
DrdI EagI EarI EciI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MslI NarI NcoI NdeI NheI
NotI NruI NsiI NspI PacI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI