(Linear) (Six-base) MAPSORT of: KIBB7533.seq  Check: 5648  from: 1  to: 513



KIBB7533.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:23  ..

AflIII A'CryG_T

Cuts at:      0     403     513 
   Size:        403     110
 
ApaI G_GGCC'C

Cuts at:      0      44     513 
   Size:         44     469
 
ApoI r'AATT_y

Cuts at:      0     389     513 
   Size:        389     124
 
AvaI C'yCGr_G

Cuts at:      0     111     513 
   Size:        111     402
 
BanII G_rGCy'C

Cuts at:      0      44     513 
   Size:         44     469
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     233     513 
   Size:        233     280
 
BglI GCCn_nnn'nGGC

Cuts at:      0     114     513 
   Size:        114     399
 
Bme1580I G_kGCm'C

Cuts at:      0      44     513 
   Size:         44     469
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     196     513 
   Size:        196     317
 
BsaBI GATnn'nnATC

Cuts at:      0     492     513 
   Size:        492      21
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     412     513 
   Size:        412     101
 
BseYI C'CCAG_C

Cuts at:      0     148     513 
   Size:        148     365
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      86     178     513 
   Size:         86      92     335

  Fragments arranged by size: 

                 335      92      86
 
Bsp1286I G_dGCh'C

Cuts at:      0      44     513 
   Size:         44     469
 
BsrBI CCG'CTC

Cuts at:      0     502     513 
   Size:        502      11
 
BstYI r'GATC_y

Cuts at:      0     345     513 
   Size:        345     168
 
Bsu36I CC'TnA_GG

Cuts at:      0     143     513 
   Size:        143     370
 
BtsI GCAGTG_nn'

Cuts at:      0      74     513 
   Size:         74     439
 
EaeI y'GGCC_r

Cuts at:      0     317     513 
   Size:        317     196
 
EarI CTCTTCn'nnn_

Cuts at:      0     227     513 
   Size:        227     286
 
EcoO109I rG'GnC_Cy

Cuts at:      0      41     513 
   Size:         41     472
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     298     330     513 
   Size:        298      32     183

  Fragments arranged by size: 

                 298     183      32
 
HincII GTy'rAC

Cuts at:      0     401     513 
   Size:        401     112
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     487     513 
   Size:        487      26
 
MspA1I CmG'CkG

Cuts at:      0      26     513 
   Size:         26     487
 
NspI r_CATG'y

Cuts at:      0     407     513 
   Size:        407     106
 
PasI CC'CwG_GG

Cuts at:      0     482     513 
   Size:        482      31
 
PciI A'CATG_T

Cuts at:      0     403     513 
   Size:        403     110
 
PspOMI G'GGCC_C

Cuts at:      0      40     513 
   Size:         40     473
 
PvuII CAG'CTG

Cuts at:      0      26     513 
   Size:         26     487
 
SmaI CCC'GGG

Cuts at:      0     113     513 
   Size:        113     400
 
SspI AAT'ATT

Cuts at:      0     509     513 
   Size:        509       4
 
StyI C'CwwG_G

Cuts at:      0     269     513 
   Size:        269     244
 
XmaI C'CCGG_G

Cuts at:      0     111     513 
   Size:        111     402
 
XmnI GAAnn'nnTTC

Cuts at:      0      13     513 
   Size:         13     500
 

 Enzymes that do cut:

   AflIII     ApaI     ApoI     AvaI    BanII     BbsI     BglI Bme1580I
     BmrI    BsaBI    BseRI    BseYI     BsgI Bsp1286I    BsrBI    BstYI
   Bsu36I     BtsI     EaeI     EarI EcoO109I    Hin4I   HincII     MmeI
   MspA1I     NspI     PasI     PciI   PspOMI    PvuII     SmaI     SspI
     StyI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI
    BsaXI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I     BtgI    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     MslI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     ZraI