(Linear) (Six-base) MAPSORT of: KIBB7526.seq  Check: 8391  from: 1  to: 753



KIBB7526.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:08  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     406     733     753 
   Size:         88     318     327      20

  Fragments arranged by size: 

                 327     318      88      20
 
AlwNI CAG_nnn'CTG

Cuts at:      0      66     595     753 
   Size:         66     529     158

  Fragments arranged by size: 

                 529     158      66
 
ApaI G_GGCC'C

Cuts at:      0     647     753 
   Size:        647     106
 
ApoI r'AATT_y

Cuts at:      0     121     164     753 
   Size:        121      43     589

  Fragments arranged by size: 

                 589     121      43
 
AseI AT'TA_AT

Cuts at:      0     626     753 
   Size:        626     127
 
BanI G'GyrC_C

Cuts at:      0      18     753 
   Size:         18     735
 
BanII G_rGCy'C

Cuts at:      0     647     753 
   Size:        647     106
 
BbeI G_GCGC'C

Cuts at:      0      22     753 
   Size:         22     731
 
Bme1580I G_kGCm'C

Cuts at:      0     647     753 
   Size:        647     106
 
BsaHI Gr'CG_yC

Cuts at:      0      19     753 
   Size:         19     734
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     240     270     753 
   Size:        240      30     483

  Fragments arranged by size: 

                 483     240      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     245     753 
   Size:        245     508
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     574     753 
   Size:        574     179
 
Bsp1286I G_dGCh'C

Cuts at:      0     647     753 
   Size:        647     106
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     604     753 
   Size:        604     149
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     672     753 
   Size:        672      81
 
BstYI r'GATC_y

Cuts at:      0      62     753 
   Size:         62     691
 
EarI CTCTTCn'nnn_

Cuts at:      0     709     753 
   Size:        709      44
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     406     733     753 
   Size:         88     318     327      20

  Fragments arranged by size: 

                 327     318      88      20
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     413     753 
   Size:        413     340
 
EcoO109I rG'GnC_Cy

Cuts at:      0     334     406     753 
   Size:        334      72     347

  Fragments arranged by size: 

                 347     334      72
 
EcoRV GAT'ATC

Cuts at:      0     282     753 
   Size:        282     471
 
HaeII r_GCGC'y

Cuts at:      0      22     753 
   Size:         22     731
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       6      38     206     238     753 
   Size:          6      32     168      32     515

  Fragments arranged by size: 

                 515     168      32      32       6
 
KasI G'GCGC_C

Cuts at:      0      18     753 
   Size:         18     735
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      98     753 
   Size:         98     655
 
MslI CAynn'nnrTG

Cuts at:      0     670     728     753 
   Size:        670      58      25

  Fragments arranged by size: 

                 670      58      25
 
NarI GG'CG_CC

Cuts at:      0      19     753 
   Size:         19     734
 
NdeI CA'TA_TG

Cuts at:      0     327     753 
   Size:        327     426
 
PacI TTA_AT'TAA

Cuts at:      0     398     753 
   Size:        398     355
 
PpuMI rG'GwC_Cy

Cuts at:      0     334     406     753 
   Size:        334      72     347

  Fragments arranged by size: 

                 347     334      72
 
PsiI TTA'TAA

Cuts at:      0     106     753 
   Size:        106     647
 
PspOMI G'GGCC_C

Cuts at:      0     643     753 
   Size:        643     110
 
PstI C_TGCA'G

Cuts at:      0     609     753 
   Size:        609     144
 
SfcI C'TryA_G

Cuts at:      0     378     476     605     753 
   Size:        378      98     129     148

  Fragments arranged by size: 

                 378     148     129      98
 
SfoI GGC'GCC

Cuts at:      0      20     753 
   Size:         20     733
 
SpeI A'CTAG_T

Cuts at:      0     423     753 
   Size:        423     330
 
SspI AAT'ATT

Cuts at:      0     654     753 
   Size:        654      99
 
StyI C'CwwG_G

Cuts at:      0      47      97     414     753 
   Size:         47      50     317     339

  Fragments arranged by size: 

                 339     317      50      47
 

 Enzymes that do cut:

     AcuI    AlwNI     ApaI     ApoI     AseI     BanI    BanII     BbeI
 Bme1580I    BsaHI    BsaXI    BseRI     BsgI Bsp1286I   BstAPI    BstXI
    BstYI     EarI  Eco57MI    EcoNI EcoO109I    EcoRV    HaeII    Hin4I
     KasI     MmeI     MslI     NarI     NdeI     PacI    PpuMI     PsiI
   PspOMI     PstI     SfcI     SfoI     SpeI     SspI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     AscI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaWI
    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI
   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoRI
     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MscI   MspA1I     NaeI     NcoI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SphI
     SrfI     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI