(Linear) (Six-base) MAPSORT of: KIBB7523.seq  Check: 950  from: 1  to: 432



KIBB7523.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     132     432 
   Size:        132     300
 
ApaLI G'TGCA_C

Cuts at:      0     399     432 
   Size:        399      33
 
BamHI G'GATC_C

Cuts at:      0     152     432 
   Size:        152     280
 
Bme1580I G_kGCm'C

Cuts at:      0     365     403     432 
   Size:        365      38      29

  Fragments arranged by size: 

                 365      38      29
 
BseYI C'CCAG_C

Cuts at:      0      41     432 
   Size:         41     391
 
BsiHKAI G_wGCw'C

Cuts at:      0     403     432 
   Size:        403      29
 
BsmI GAATG_Cn'

Cuts at:      0     314     432 
   Size:        314     118
 
Bsp1286I G_dGCh'C

Cuts at:      0     365     403     432 
   Size:        365      38      29

  Fragments arranged by size: 

                 365      38      29
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      41     432 
   Size:         41     391
 
BstYI r'GATC_y

Cuts at:      0     152     432 
   Size:        152     280
 
DraIII CAC_nnn'GTG

Cuts at:      0     409     432 
   Size:        409      23
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     132     432 
   Size:        132     300
 
SspI AAT'ATT

Cuts at:      0     229     432 
   Size:        229     203
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     121     153     432 
   Size:        121      32     279

  Fragments arranged by size: 

                 279     121      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     372     432 
   Size:        372      60
 

 Enzymes that do cut:

     AcuI    ApaLI    BamHI Bme1580I    BseYI  BsiHKAI     BsmI Bsp1286I
    BstXI    BstYI   DraIII  Eco57MI     SspI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     StuI     StyI     SwaI    TaqII     TatI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI