Restriction map for
pF1KB7506
(Linear) (Six-base) MAPSORT of: KIBB7506.seq Check: 6676 from: 1 to: 720
KIBB7506.orf
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 547 720
Size: 547 173
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 490 522 720
Size: 490 32 198
Fragments arranged by size:
490 198 32
AlwNI CAG_nnn'CTG
Cuts at: 0 423 720
Size: 423 297
ApaI G_GGCC'C
Cuts at: 0 444 720
Size: 444 276
ApoI r'AATT_y
Cuts at: 0 534 720
Size: 534 186
AvaI C'yCGr_G
Cuts at: 0 274 433 720
Size: 274 159 287
Fragments arranged by size:
287 274 159
BanI G'GyrC_C
Cuts at: 0 323 720
Size: 323 397
BanII G_rGCy'C
Cuts at: 0 444 720
Size: 444 276
BbeI G_GCGC'C
Cuts at: 0 327 720
Size: 327 393
BbsI GAAGACnn'nnnn_
Cuts at: 0 42 720
Size: 42 678
BglI GCCn_nnn'nGGC
Cuts at: 0 448 720
Size: 448 272
BglII A'GATC_T
Cuts at: 0 485 720
Size: 485 235
Bme1580I G_kGCm'C
Cuts at: 0 444 720
Size: 444 276
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 300 720
Size: 300 420
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 126 357 720
Size: 126 231 363
Fragments arranged by size:
363 231 126
BsaHI Gr'CG_yC
Cuts at: 0 35 324 720
Size: 35 289 396
Fragments arranged by size:
396 289 35
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 490 520 720
Size: 490 30 200
Fragments arranged by size:
490 200 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 678 720
Size: 678 42
BseYI C'CCAG_C
Cuts at: 0 443 720
Size: 443 277
BsiEI CG_ry'CG
Cuts at: 0 30 720
Size: 30 690
BsiHKAI G_wGCw'C
Cuts at: 0 263 720
Size: 263 457
Bsp1286I G_dGCh'C
Cuts at: 0 263 444 720
Size: 263 181 276
Fragments arranged by size:
276 263 181
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 261 720
Size: 261 459
BsrBI CCG'CTC
Cuts at: 0 321 720
Size: 321 399
BstYI r'GATC_y
Cuts at: 0 485 615 720
Size: 485 130 105
Fragments arranged by size:
485 130 105
Bsu36I CC'TnA_GG
Cuts at: 0 266 329 720
Size: 266 63 391
Fragments arranged by size:
391 266 63
BtgI C'CryG_G
Cuts at: 0 224 295 720
Size: 224 71 425
Fragments arranged by size:
425 224 71
EaeI y'GGCC_r
Cuts at: 0 27 153 720
Size: 27 126 567
Fragments arranged by size:
567 126 27
EagI C'GGCC_G
Cuts at: 0 27 720
Size: 27 693
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 126 357 547 720
Size: 126 231 190 173
Fragments arranged by size:
231 190 173 126
EcoNI CCTnn'n_nnAGG
Cuts at: 0 141 720
Size: 141 579
EcoO109I rG'GnC_Cy
Cuts at: 0 333 440 569 632 720
Size: 333 107 129 63 88
Fragments arranged by size:
333 129 107 88 63
EcoRI G'AATT_C
Cuts at: 0 534 720
Size: 534 186
HaeII r_GCGC'y
Cuts at: 0 232 327 430 720
Size: 232 95 103 290
Fragments arranged by size:
290 232 103 95
KasI G'GCGC_C
Cuts at: 0 323 720
Size: 323 397
MscI TGG'CCA
Cuts at: 0 155 720
Size: 155 565
MspA1I CmG'CkG
Cuts at: 0 226 297 384 447 720
Size: 226 71 87 63 273
Fragments arranged by size:
273 226 87 71 63
NarI GG'CG_CC
Cuts at: 0 324 720
Size: 324 396
PasI CC'CwG_GG
Cuts at: 0 562 720
Size: 562 158
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 490 522 720
Size: 490 32 198
Fragments arranged by size:
490 198 32
PpuMI rG'GwC_Cy
Cuts at: 0 569 632 720
Size: 569 63 88
Fragments arranged by size:
569 88 63
PspOMI G'GGCC_C
Cuts at: 0 440 720
Size: 440 280
PspXI vC'TCGA_Gb
Cuts at: 0 274 720
Size: 274 446
PstI C_TGCA'G
Cuts at: 0 258 383 389 720
Size: 258 125 6 331
Fragments arranged by size:
331 258 125 6
PvuII CAG'CTG
Cuts at: 0 384 447 720
Size: 384 63 273
Fragments arranged by size:
384 273 63
SacII CC_GC'GG
Cuts at: 0 227 298 720
Size: 227 71 422
Fragments arranged by size:
422 227 71
SanDI GG'GwC_CC
Cuts at: 0 632 720
Size: 632 88
SfcI C'TryA_G
Cuts at: 0 254 379 385 720
Size: 254 125 6 335
Fragments arranged by size:
335 254 125 6
SfoI GGC'GCC
Cuts at: 0 325 720
Size: 325 395
SmlI C'TyrA_G
Cuts at: 0 274 720
Size: 274 446
StyI C'CwwG_G
Cuts at: 0 623 720
Size: 623 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 484 720
Size: 484 236
XhoI C'TCGA_G
Cuts at: 0 274 720
Size: 274 446
Enzymes that do cut:
AcuI AloI AlwNI ApaI ApoI AvaI BanI BanII
BbeI BbsI BglI BglII Bme1580I BplI BpmI BsaHI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspMI BsrBI
BstYI Bsu36I BtgI EaeI EagI Eco57MI EcoNI EcoO109I
EcoRI HaeII KasI MscI MspA1I NarI PasI PpiI
PpuMI PspOMI PspXI PstI PvuII SacII SanDI SfcI
SfoI SmlI StyI TaqII XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI
BlpI BmgBI BmrI BmtI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaWI BsgI BsiWI BsmI BsmBI BspEI BspHI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EarI EciI EcoICRI EcoRV FalI FseI FspI
FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MslI NaeI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PflMI PfoI PmeI
PmlI PshAI PsiI PsrI PvuI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI