(Linear) (Six-base) MAPSORT of: KIBB7396.seq  Check: 2127  from: 1  to: 576



KIBB7396.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AflIII A'CryG_T

Cuts at:      0      92     576 
   Size:         92     484
 
AgeI A'CCGG_T

Cuts at:      0     205     576 
   Size:        205     371
 
ApoI r'AATT_y

Cuts at:      0      75     378     576 
   Size:         75     303     198

  Fragments arranged by size: 

                 303     198      75
 
BanI G'GyrC_C

Cuts at:      0     202     263     576 
   Size:        202      61     313

  Fragments arranged by size: 

                 313     202      61
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     491     576 
   Size:        491      85
 
BglI GCCn_nnn'nGGC

Cuts at:      0     535     576 
   Size:        535      41
 
BmgBI CAC'GTC

Cuts at:      0     145     390     576 
   Size:        145     245     186

  Fragments arranged by size: 

                 245     186     145
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     428     576 
   Size:        428     148
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     464     576 
   Size:        464     112
 
BsaWI w'CCGG_w

Cuts at:      0     205     576 
   Size:        205     371
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     140     170     451     481     576 
   Size:        140      30     281      30      95

  Fragments arranged by size: 

                 281     140      95      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     535     576 
   Size:        535      41
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     135     440     576 
   Size:        135     305     136

  Fragments arranged by size: 

                 305     136     135
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     227     576 
   Size:        227     349
 
BsrBI CCG'CTC

Cuts at:      0     151     576 
   Size:        151     425
 
BsrFI r'CCGG_y

Cuts at:      0     205     576 
   Size:        205     371
 
DraIII CAC_nnn'GTG

Cuts at:      0     114     576 
   Size:        114     462
 
EarI CTCTTCn'nnn_

Cuts at:      0     512     576 
   Size:        512      64
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     464     576 
   Size:        464     112
 
EcoO109I rG'GnC_Cy

Cuts at:      0     324     576 
   Size:        324     252
 
HindIII A'AGCT_T

Cuts at:      0     556     576 
   Size:        556      20
 
NspI r_CATG'y

Cuts at:      0      96     215     576 
   Size:         96     119     361

  Fragments arranged by size: 

                 361     119      96
 
PasI CC'CwG_GG

Cuts at:      0     327     576 
   Size:        327     249
 
PciI A'CATG_T

Cuts at:      0      92     576 
   Size:         92     484
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     508     576 
   Size:        508      68
 
SgrAI Cr'CCGG_yG

Cuts at:      0     205     576 
   Size:        205     371
 
SphI G_CATG'C

Cuts at:      0     215     576 
   Size:        215     361
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     386     576 
   Size:        386     190
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     451     483     576 
   Size:        451      32      93

  Fragments arranged by size: 

                 451      93      32
 

 Enzymes that do cut:

   AflIII     AgeI     ApoI     BanI     BbsI     BglI    BmgBI     BplI
     BpmI    BsaWI    BsaXI    BseRI    BsmBI    BspMI    BsrBI    BsrFI
   DraIII     EarI  Eco57MI EcoO109I  HindIII     NspI     PasI     PciI
    PflMI    SgrAI     SphI    TaqII     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I
     BmrI     BmtI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI Bsp1286I    BspEI
    BspHI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI     SmaI     SmlI
    SnaBI     SpeI     SrfI     SspI     StuI     StyI     SwaI     TatI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI