(Linear) (Six-base) MAPSORT of: KIBB7395.seq  Check: 176  from: 1  to: 663



KIBB7395.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

Acc65I G'GTAC_C

Cuts at:      0     293     663 
   Size:        293     370
 
AvaI C'yCGr_G

Cuts at:      0     541     663 
   Size:        541     122
 
BanI G'GyrC_C

Cuts at:      0     293     663 
   Size:        293     370
 
BclI T'GATC_A

Cuts at:      0     393     663 
   Size:        393     270
 
BlpI GC'TnA_GC

Cuts at:      0     625     663 
   Size:        625      38
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      50     663 
   Size:         50     613
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      66     541     663 
   Size:         66     475     122

  Fragments arranged by size: 

                 475     122      66
 
BsiHKAI G_wGCw'C

Cuts at:      0     631     663 
   Size:        631      32
 
Bsp1286I G_dGCh'C

Cuts at:      0     631     663 
   Size:        631      32
 
BsrDI GCAATG_nn'

Cuts at:      0      24     663 
   Size:         24     639
 
BtsI GCAGTG_nn'

Cuts at:      0     610     663 
   Size:        610      53
 
DraI TTT'AAA

Cuts at:      0     468     663 
   Size:        468     195
 
EarI CTCTTCn'nnn_

Cuts at:      0     410     542     663 
   Size:        410     132     121

  Fragments arranged by size: 

                 410     132     121
 
KpnI G_GTAC'C

Cuts at:      0     297     663 
   Size:        297     366
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     114     663 
   Size:        114     549
 
MspA1I CmG'CkG

Cuts at:      0     624     663 
   Size:        624      39
 
PsiI TTA'TAA

Cuts at:      0     587     663 
   Size:        587      76
 
PspXI vC'TCGA_Gb

Cuts at:      0     541     663 
   Size:        541     122
 
PstI C_TGCA'G

Cuts at:      0     308     491     617     663 
   Size:        308     183     126      46

  Fragments arranged by size: 

                 308     183     126      46
 
PvuII CAG'CTG

Cuts at:      0     624     663 
   Size:        624      39
 
SfcI C'TryA_G

Cuts at:      0     304     487     613     663 
   Size:        304     183     126      50

  Fragments arranged by size: 

                 304     183     126      50
 
SmlI C'TyrA_G

Cuts at:      0      65     541     663 
   Size:         65     476     122

  Fragments arranged by size: 

                 476     122      65
 
StyI C'CwwG_G

Cuts at:      0     166     663 
   Size:        166     497
 
TatI w'GTAC_w

Cuts at:      0     115     663 
   Size:        115     548
 
XhoI C'TCGA_G

Cuts at:      0     541     663 
   Size:        541     122
 

 Enzymes that do cut:

   Acc65I     AvaI     BanI     BclI     BlpI    BpuEI    BseRI  BsiHKAI
 Bsp1286I    BsrDI     BtsI     DraI     EarI     KpnI     MmeI   MspA1I
     PsiI    PspXI     PstI    PvuII     SfcI     SmlI     StyI     TatI
     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     MfeI     MluI     MscI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI   PspOMI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI
     XmaI     XmnI     ZraI