(Linear) (Six-base) MAPSORT of: KIBB7394.seq  Check: 4030  from: 1  to: 612



KIBB7394.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:00  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     267     612 
   Size:        267     345
 
AfeI AGC'GCT

Cuts at:      0     100     612 
   Size:        100     512
 
ApoI r'AATT_y

Cuts at:      0     604     612 
   Size:        604       8
 
BanI G'GyrC_C

Cuts at:      0      74     431     449     612 
   Size:         74     357      18     163

  Fragments arranged by size: 

                 357     163      74      18
 
BanII G_rGCy'C

Cuts at:      0      58     180     389     612 
   Size:         58     122     209     223

  Fragments arranged by size: 

                 223     209     122      58
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     349     612 
   Size:        349     263
 
BlpI GC'TnA_GC

Cuts at:      0     491     612 
   Size:        491     121
 
Bme1580I G_kGCm'C

Cuts at:      0     373     612 
   Size:        373     239
 
BmgBI CAC'GTC

Cuts at:      0     519     612 
   Size:        519      93
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     362     586     612 
   Size:        362     224      26

  Fragments arranged by size: 

                 362     224      26
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     352     612 
   Size:        352     260
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     254     612 
   Size:        254     358
 
BseYI C'CCAG_C

Cuts at:      0     244     274     478     612 
   Size:        244      30     204     134

  Fragments arranged by size: 

                 244     204     134      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     240     612 
   Size:        240     372
 
BsiEI CG_ry'CG

Cuts at:      0     439     612 
   Size:        439     173
 
BsiHKAI G_wGCw'C

Cuts at:      0      58     140     612 
   Size:         58      82     472

  Fragments arranged by size: 

                 472      82      58
 
Bsp1286I G_dGCh'C

Cuts at:      0      58     140     180     373     389     612 
   Size:         58      82      40     193      16     223

  Fragments arranged by size: 

                 223     193      82      58      40      16
 
BsrGI T'GTAC_A

Cuts at:      0     227     612 
   Size:        227     385
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     581     612 
   Size:        581      31
 
EcoICRI GAG'CTC

Cuts at:      0      56     612 
   Size:         56     556
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     267     612 
   Size:        267     345
 
HaeII r_GCGC'y

Cuts at:      0     102     612 
   Size:        102     510
 
NdeI CA'TA_TG

Cuts at:      0     338     612 
   Size:        338     274
 
NspI r_CATG'y

Cuts at:      0     133     612 
   Size:        133     479
 
PasI CC'CwG_GG

Cuts at:      0      60     191     612 
   Size:         60     131     421

  Fragments arranged by size: 

                 421     131      60
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     172     304     612 
   Size:        172     132     308

  Fragments arranged by size: 

                 308     172     132
 
SacI G_AGCT'C

Cuts at:      0      58     612 
   Size:         58     554
 
SphI G_CATG'C

Cuts at:      0     133     612 
   Size:        133     479
 
StyI C'CwwG_G

Cuts at:      0     298     315     612 
   Size:        298      17     297

  Fragments arranged by size: 

                 298     297      17
 
TatI w'GTAC_w

Cuts at:      0     227     612 
   Size:        227     385
 
Tth111I GACn'n_nGTC

Cuts at:      0     440     612 
   Size:        440     172
 
XmnI GAAnn'nnTTC

Cuts at:      0     346     612 
   Size:        346     266
 

 Enzymes that do cut:

     AcuI     AfeI     ApoI     BanI    BanII     BbsI     BlpI Bme1580I
    BmgBI     BmrI     BsaI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
 Bsp1286I    BsrGI    BtgZI  EcoICRI  Eco57MI    HaeII     NdeI     NspI
     PasI    PflMI     SacI     SphI     StyI     TatI  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BmtI     BplI
     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SrfI     SspI     StuI     SwaI    TaqII     TstI     XbaI
     XcmI     XhoI     XmaI     ZraI