Restriction map for
pF1KB7392
(Linear) (Six-base) MAPSORT of: KIBB7392.seq Check: 9776 from: 1 to: 1020
KIBB7392.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AatII G_ACGT'C
Cuts at: 0 139 1020
Size: 139 881
ApoI r'AATT_y
Cuts at: 0 38 544 1020
Size: 38 506 476
Fragments arranged by size:
506 476 38
AvaI C'yCGr_G
Cuts at: 0 175 308 770 1020
Size: 175 133 462 250
Fragments arranged by size:
462 250 175 133
AvrII C'CTAG_G
Cuts at: 0 804 1020
Size: 804 216
BanI G'GyrC_C
Cuts at: 0 858 1020
Size: 858 162
BanII G_rGCy'C
Cuts at: 0 111 290 704 909 1020
Size: 111 179 414 205 111
Fragments arranged by size:
414 205 179 111 111
BbeI G_GCGC'C
Cuts at: 0 862 1020
Size: 862 158
BbsI GAAGACnn'nnnn_
Cuts at: 0 242 881 1020
Size: 242 639 139
Fragments arranged by size:
639 242 139
BbvCI CC'TCA_GC
Cuts at: 0 869 1020
Size: 869 151
BclI T'GATC_A
Cuts at: 0 95 1020
Size: 95 925
BmgBI CAC'GTC
Cuts at: 0 93 1020
Size: 93 927
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 955 1020
Size: 955 65
Bpu10I CC'TnA_GC
Cuts at: 0 869 1020
Size: 869 151
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 1020
Size: 283 737
BsaHI Gr'CG_yC
Cuts at: 0 136 859 1020
Size: 136 723 161
Fragments arranged by size:
723 161 136
BsaWI w'CCGG_w
Cuts at: 0 660 1020
Size: 660 360
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 953 983 1020
Size: 953 30 37
Fragments arranged by size:
953 37 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 208 217 220 223 226 298 1020
Size: 208 9 3 3 3 72 722
Fragments arranged by size:
722 208 72 9 3 3 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 797 1020
Size: 797 223
BsiHKAI G_wGCw'C
Cuts at: 0 290 1020
Size: 290 730
Bsp1286I G_dGCh'C
Cuts at: 0 111 290 704 909 1020
Size: 111 179 414 205 111
Fragments arranged by size:
414 205 179 111 111
BspEI T'CCGG_A
Cuts at: 0 660 1020
Size: 660 360
BsrBI CCG'CTC
Cuts at: 0 315 1020
Size: 315 705
BsrFI r'CCGG_y
Cuts at: 0 947 1020
Size: 947 73
BssSI C'ACGA_G
Cuts at: 0 280 1020
Size: 280 740
BstYI r'GATC_y
Cuts at: 0 808 1020
Size: 808 212
BtgI C'CryG_G
Cuts at: 0 595 1020
Size: 595 425
EaeI y'GGCC_r
Cuts at: 0 598 1020
Size: 598 422
EarI CTCTTCn'nnn_
Cuts at: 0 97 185 194 212 218 711 937 1020
Size: 97 88 9 18 6 493 226 83
Fragments arranged by size:
493 226 97 88 83 18 9 6
EcoICRI GAG'CTC
Cuts at: 0 288 1020
Size: 288 732
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 955 1020
Size: 955 65
EcoRI G'AATT_C
Cuts at: 0 38 1020
Size: 38 982
EcoRV GAT'ATC
Cuts at: 0 67 1020
Size: 67 953
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 58 1020
Size: 58 962
HaeII r_GCGC'y
Cuts at: 0 862 869 1020
Size: 862 7 151
Fragments arranged by size:
862 151 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 478 510 766 798 1000 1020
Size: 478 32 256 32 202 20
Fragments arranged by size:
478 256 202 32 32 20
KasI G'GCGC_C
Cuts at: 0 858 1020
Size: 858 162
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 582 1020
Size: 582 438
MslI CAynn'nnrTG
Cuts at: 0 421 1020
Size: 421 599
MspA1I CmG'CkG
Cuts at: 0 87 636 1020
Size: 87 549 384
Fragments arranged by size:
549 384 87
NarI GG'CG_CC
Cuts at: 0 859 1020
Size: 859 161
PflMI CCAn_nnn'nTGG
Cuts at: 0 22 246 1020
Size: 22 224 774
Fragments arranged by size:
774 224 22
SacI G_AGCT'C
Cuts at: 0 290 1020
Size: 290 730
SapI GCTCTTCn'nnn_
Cuts at: 0 937 1020
Size: 937 83
ScaI AGT'ACT
Cuts at: 0 615 1020
Size: 615 405
SfoI GGC'GCC
Cuts at: 0 860 1020
Size: 860 160
SmaI CCC'GGG
Cuts at: 0 310 1020
Size: 310 710
SmlI C'TyrA_G
Cuts at: 0 298 1020
Size: 298 722
SspI AAT'ATT
Cuts at: 0 465 1020
Size: 465 555
StuI AGG'CCT
Cuts at: 0 755 1020
Size: 755 265
StyI C'CwwG_G
Cuts at: 0 804 1020
Size: 804 216
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 831 1020
Size: 831 189
TatI w'GTAC_w
Cuts at: 0 613 1020
Size: 613 407
XbaI T'CTAG_A
Cuts at: 0 325 375 1020
Size: 325 50 645
Fragments arranged by size:
645 325 50
XmaI C'CCGG_G
Cuts at: 0 308 1020
Size: 308 712
ZraI GAC'GTC
Cuts at: 0 137 1020
Size: 137 883
Enzymes that do cut:
AatII ApoI AvaI AvrII BanI BanII BbeI BbsI
BbvCI BclI BmgBI BpmI Bpu10I BpuEI BsaHI BsaWI
BsaXI BseRI BsgI BsiHKAI Bsp1286I BspEI BsrBI BsrFI
BssSI BstYI BtgI EaeI EarI EcoICRI Eco57MI EcoRI
EcoRV FalI HaeII Hin4I KasI MmeI MslI MspA1I
NarI PflMI SacI SapI ScaI SfoI SmaI SmlI
SspI StuI StyI TaqII TatI XbaI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
AscI AseI AsiSI BaeI BamHI BcgI BciVI BfrBI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BsaI
BsaAI BsaBI BseYI BsiEI BsiWI BsmI BsmBI BspHI
BspMI BsrDI BsrGI BssHII BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoNI EcoO109I FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MscI NaeI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacII SalI SanDI SbfI SexAI SfcI SfiI
SgfI SgrAI SnaBI SpeI SphI SrfI SwaI TstI
Tth111I XcmI XhoI XmnI