(Linear) (Six-base) MAPSORT of: KIBB7391.seq  Check: 6292  from: 1  to: 693



KIBB7391.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:00  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     478     693 
   Size:        478     215
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     693 
   Size:         46     647
 
AflIII A'CryG_T

Cuts at:      0     280     693 
   Size:        280     413
 
AlwNI CAG_nnn'CTG

Cuts at:      0     354     693 
   Size:        354     339
 
AvrII C'CTAG_G

Cuts at:      0     229     693 
   Size:        229     464
 
BanI G'GyrC_C

Cuts at:      0     687     693 
   Size:        687       6
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     456     490     693 
   Size:        456      34     203

  Fragments arranged by size: 

                 456     203      34
 
BfrBI ATG'CAT

Cuts at:      0     421     693 
   Size:        421     272
 
Bme1580I G_kGCm'C

Cuts at:      0     692     693 
   Size:        692       1
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     590     693 
   Size:        590     103
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      53     693 
   Size:         53     640
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     415     693 
   Size:        415     278
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     153     288     693 
   Size:        153     135     405

  Fragments arranged by size: 

                 405     153     135
 
BsmI GAATG_Cn'

Cuts at:      0     100     693 
   Size:        100     593
 
Bsp1286I G_dGCh'C

Cuts at:      0     692     693 
   Size:        692       1
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     478     693 
   Size:        478     215
 
BsrFI r'CCGG_y

Cuts at:      0     454     693 
   Size:        454     239
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      37     693 
   Size:         37     656
 
BstYI r'GATC_y

Cuts at:      0     188     680     693 
   Size:        188     492      13

  Fragments arranged by size: 

                 492     188      13
 
Bsu36I CC'TnA_GG

Cuts at:      0     206     693 
   Size:        206     487
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     660     693 
   Size:        660      33
 
EaeI y'GGCC_r

Cuts at:      0     462     597     693 
   Size:        462     135      96

  Fragments arranged by size: 

                 462     135      96
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     590     693 
   Size:         46     544     103

  Fragments arranged by size: 

                 544     103      46
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     166     693 
   Size:        166     527
 
MspA1I CmG'CkG

Cuts at:      0     382     693 
   Size:        382     311
 
NsiI A_TGCA'T

Cuts at:      0     423     693 
   Size:        423     270
 
NspI r_CATG'y

Cuts at:      0      82     284     693 
   Size:         82     202     409

  Fragments arranged by size: 

                 409     202      82
 
PasI CC'CwG_GG

Cuts at:      0     432     693 
   Size:        432     261
 
PciI A'CATG_T

Cuts at:      0     280     693 
   Size:        280     413
 
PfoI T'CCnGG_A

Cuts at:      0      58     693 
   Size:         58     635
 
PstI C_TGCA'G

Cuts at:      0      72     451     693 
   Size:         72     379     242

  Fragments arranged by size: 

                 379     242      72
 
SfcI C'TryA_G

Cuts at:      0      68     447     693 
   Size:         68     379     246

  Fragments arranged by size: 

                 379     246      68
 
StyI C'CwwG_G

Cuts at:      0     229     693 
   Size:        229     464
 

 Enzymes that do cut:

     AarI     AcuI   AflIII    AlwNI    AvrII     BanI     BcgI    BfrBI
 Bme1580I     BpmI     BsaI    BseRI     BsgI     BsmI Bsp1286I    BspMI
    BsrFI   BstAPI    BstYI   Bsu36I     DrdI     EaeI  Eco57MI     MmeI
   MspA1I     NsiI     NspI     PasI     PciI     PfoI     PstI     SfcI
     StyI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI     BaeI    BamHI    BanII     BbeI     BbsI    BbvCI
    BciVI     BclI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI
     BplI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseYI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     EagI
     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     MslI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     PacI    PflMI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI