Restriction map for
pF1KB7389
(Linear) (Six-base) MAPSORT of: KIBB7389.seq Check: 8302 from: 1 to: 615
KIBB7389.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AccI GT'mk_AC
Cuts at: 0 488 615
Size: 488 127
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 541 615
Size: 541 74
AlwNI CAG_nnn'CTG
Cuts at: 0 435 615
Size: 435 180
AvrII C'CTAG_G
Cuts at: 0 609 615
Size: 609 6
BanI G'GyrC_C
Cuts at: 0 409 615
Size: 409 206
BanII G_rGCy'C
Cuts at: 0 248 406 615
Size: 248 158 209
Fragments arranged by size:
248 209 158
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 457 491 615
Size: 457 34 124
Fragments arranged by size:
457 124 34
Bme1580I G_kGCm'C
Cuts at: 0 414 615
Size: 414 201
BmgBI CAC'GTC
Cuts at: 0 113 615
Size: 113 502
BmrI ACTGGGnnnn_n'
Cuts at: 0 537 615
Size: 537 78
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 574 615
Size: 262 312 41
Fragments arranged by size:
312 262 41
BsaAI yAC'GTr
Cuts at: 0 572 615
Size: 572 43
BsaBI GATnn'nnATC
Cuts at: 0 337 615
Size: 337 278
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 527 615
Size: 527 88
BsiHKAI G_wGCw'C
Cuts at: 0 248 571 615
Size: 248 323 44
Fragments arranged by size:
323 248 44
Bsp1286I G_dGCh'C
Cuts at: 0 248 406 414 571 615
Size: 248 158 8 157 44
Fragments arranged by size:
248 158 157 44 8
BstEII G'GTnAC_C
Cuts at: 0 217 615
Size: 217 398
BstXI CCAn_nnnn'nTGG
Cuts at: 0 288 615
Size: 288 327
BstYI r'GATC_y
Cuts at: 0 59 615
Size: 59 556
BtsI GCAGTG_nn'
Cuts at: 0 317 615
Size: 317 298
DraI TTT'AAA
Cuts at: 0 137 615
Size: 137 478
EaeI y'GGCC_r
Cuts at: 0 255 615
Size: 255 360
EarI CTCTTCn'nnn_
Cuts at: 0 253 381 615
Size: 253 128 234
Fragments arranged by size:
253 234 128
EcoICRI GAG'CTC
Cuts at: 0 246 615
Size: 246 369
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 541 574 615
Size: 262 279 33 41
Fragments arranged by size:
279 262 41 33
EcoO109I rG'GnC_Cy
Cuts at: 0 225 596 615
Size: 225 371 19
Fragments arranged by size:
371 225 19
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 130 615
Size: 73 57 485
Fragments arranged by size:
485 73 57
MslI CAynn'nnrTG
Cuts at: 0 286 615
Size: 286 329
MspA1I CmG'CkG
Cuts at: 0 519 615
Size: 519 96
NspI r_CATG'y
Cuts at: 0 41 615
Size: 41 574
PmlI CAC'GTG
Cuts at: 0 572 615
Size: 572 43
PpuMI rG'GwC_Cy
Cuts at: 0 225 596 615
Size: 225 371 19
Fragments arranged by size:
371 225 19
PvuII CAG'CTG
Cuts at: 0 519 615
Size: 519 96
SacI G_AGCT'C
Cuts at: 0 248 615
Size: 248 367
SapI GCTCTTCn'nnn_
Cuts at: 0 253 615
Size: 253 362
SfcI C'TryA_G
Cuts at: 0 105 615
Size: 105 510
StyI C'CwwG_G
Cuts at: 0 609 615
Size: 609 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 77 370 615
Size: 77 293 245
Fragments arranged by size:
293 245 77
TatI w'GTAC_w
Cuts at: 0 299 615
Size: 299 316
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 73 105 615
Size: 73 32 510
Fragments arranged by size:
510 73 32
Enzymes that do cut:
AccI AcuI AlwNI AvrII BanI BanII BcgI Bme1580I
BmgBI BmrI BpmI BsaAI BsaBI BsgI BsiHKAI Bsp1286I
BstEII BstXI BstYI BtsI DraI EaeI EarI EcoICRI
Eco57MI EcoO109I MmeI MslI MspA1I NspI PmlI PpuMI
PvuII SacI SapI SfcI StyI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI BaeI BamHI BbeI BbsI BbvCI
BciVI BclI BfrBI BglI BglII BlpI BmtI BplI
Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstZ17I
Bsu36I BtgI BtgZI ClaI CspCI DraIII DrdI EagI
EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI