(Linear) (Six-base) MAPSORT of: KIBB7387.seq  Check: 2392  from: 1  to: 1290



KIBB7387.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:00  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     744    1080    1290 
   Size:        744     336     210

  Fragments arranged by size: 

                 744     336     210
 
AlwNI CAG_nnn'CTG

Cuts at:      0     374    1290 
   Size:        374     916
 
ApaLI G'TGCA_C

Cuts at:      0    1190    1290 
   Size:       1190     100
 
ApoI r'AATT_y

Cuts at:      0     511     557     582     878    1290 
   Size:        511      46      25     296     412

  Fragments arranged by size: 

                 511     412     296      46      25
 
AvaI C'yCGr_G

Cuts at:      0    1223    1290 
   Size:       1223      67
 
AvrII C'CTAG_G

Cuts at:      0     387    1290 
   Size:        387     903
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     160     193    1290 
   Size:        160      33    1097

  Fragments arranged by size: 

                1097     160      33
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1243    1277    1290 
   Size:       1243      34      13

  Fragments arranged by size: 

                1243      34      13
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     249     848    1290 
   Size:        249     599     442

  Fragments arranged by size: 

                 599     442     249
 
BclI T'GATC_A

Cuts at:      0     714    1290 
   Size:        714     576
 
BglII A'GATC_T

Cuts at:      0      40    1290 
   Size:         40    1250
 
Bme1580I G_kGCm'C

Cuts at:      0    1194    1290 
   Size:       1194      96
 
BmgBI CAC'GTC

Cuts at:      0     138    1290 
   Size:        138    1152
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     181     734    1290 
   Size:        181     553     556

  Fragments arranged by size: 

                 556     553     181
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     166     992    1290 
   Size:        166     826     298

  Fragments arranged by size: 

                 826     298     166
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     520    1290 
   Size:        520     770
 
BsaAI yAC'GTr

Cuts at:      0     648    1290 
   Size:        648     642
 
BsaBI GATnn'nnATC

Cuts at:      0     260     326     413    1290 
   Size:        260      66      87     877

  Fragments arranged by size: 

                 877     260      87      66
 
BsaWI w'CCGG_w

Cuts at:      0    1054    1290 
   Size:       1054     236
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      19     106    1290 
   Size:         19      87    1184

  Fragments arranged by size: 

                1184      87      19
 
BsiHKAI G_wGCw'C

Cuts at:      0    1194    1290 
   Size:       1194      96
 
BsmI GAATG_Cn'

Cuts at:      0     936    1290 
   Size:        936     354
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     128    1290 
   Size:        128    1162
 
Bsp1286I G_dGCh'C

Cuts at:      0    1194    1290 
   Size:       1194      96
 
BsrDI GCAATG_nn'

Cuts at:      0     915    1290 
   Size:        915     375
 
BstYI r'GATC_y

Cuts at:      0      40    1290 
   Size:         40    1250
 
BtgI C'CryG_G

Cuts at:      0      74    1290 
   Size:         74    1216
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     897     932    1290 
   Size:        897      35     358

  Fragments arranged by size: 

                 897     358      35
 
EaeI y'GGCC_r

Cuts at:      0      71    1290 
   Size:         71    1219
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     166     744     992    1080    1290 
   Size:        166     578     248      88     210

  Fragments arranged by size: 

                 578     248     210     166      88
 
EcoRI G'AATT_C

Cuts at:      0     557     582     878    1290 
   Size:        557      25     296     412

  Fragments arranged by size: 

                 557     412     296      25
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     272     304     618     650     843     875    1095    1127 
   Size:        272      32     314      32     193      32     220      32

Cuts at:   1127    1290 
   Size:        163

  Fragments arranged by size: 

                 314     272     220     193     163      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     299    1290 
   Size:        299     991
 
HindIII A'AGCT_T

Cuts at:      0    1007    1290 
   Size:       1007     283
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     191    1290 
   Size:        191    1099
 
MslI CAynn'nnrTG

Cuts at:      0     266    1290 
   Size:        266    1024
 
MspA1I CmG'CkG

Cuts at:      0     105    1290 
   Size:        105    1185
 
PstI C_TGCA'G

Cuts at:      0     732    1290 
   Size:        732     558
 
PvuII CAG'CTG

Cuts at:      0     105    1290 
   Size:        105    1185
 
SfcI C'TryA_G

Cuts at:      0     153     728    1290 
   Size:        153     575     562

  Fragments arranged by size: 

                 575     562     153
 
SmlI C'TyrA_G

Cuts at:      0     499    1290 
   Size:        499     791
 
SnaBI TAC'GTA

Cuts at:      0     648    1290 
   Size:        648     642
 
SspI AAT'ATT

Cuts at:      0     315    1290 
   Size:        315     975
 
StuI AGG'CCT

Cuts at:      0     757    1290 
   Size:        757     533
 
StyI C'CwwG_G

Cuts at:      0     387    1290 
   Size:        387     903
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     219    1290 
   Size:        219    1071
 
XmnI GAAnn'nnTTC

Cuts at:      0     561     757     925    1290 
   Size:        561     196     168     365

  Fragments arranged by size: 

                 561     365     196     168
 

 Enzymes that do cut:

     AcuI    AlwNI    ApaLI     ApoI     AvaI    AvrII     BaeI     BcgI
    BciVI     BclI    BglII Bme1580I    BmgBI     BmrI     BpmI    BpuEI
    BsaAI    BsaBI    BsaWI    BseRI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BsrDI    BstYI     BtgI    CspCI     EaeI  Eco57MI    EcoRI    Hin4I
   HincII  HindIII     MmeI     MslI   MspA1I     PstI    PvuII     SfcI
     SmlI    SnaBI     SspI     StuI     StyI    TaqII     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI     AscI     AseI
    AsiSI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI    BfrBI
     BglI     BlpI     BmtI     BplI   Bpu10I     BsaI    BsaHI    BsaXI
    BseYI     BsgI    BsiEI    BsiWI    BspEI    BspHI    BspMI    BsrBI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI     DraI   DraIII     DrdI
     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FalI
     FseI     FspI    FspAI    HaeII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SpeI     SphI     SrfI     SwaI     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI