Restriction map for
pF1KB7385
(Linear) (Six-base) MAPSORT of: KIBB7385.seq Check: 4529 from: 1 to: 951
KIBB7385.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AccI GT'mk_AC
Cuts at: 0 226 951
Size: 226 725
AflIII A'CryG_T
Cuts at: 0 75 571 951
Size: 75 496 380
Fragments arranged by size:
496 380 75
AleI CACnn'nnGTG
Cuts at: 0 743 951
Size: 743 208
BlpI GC'TnA_GC
Cuts at: 0 607 951
Size: 607 344
BmgBI CAC'GTC
Cuts at: 0 78 951
Size: 78 873
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 803 951
Size: 85 718 148
Fragments arranged by size:
718 148 85
BsaAI yAC'GTr
Cuts at: 0 375 951
Size: 375 576
BsaHI Gr'CG_yC
Cuts at: 0 290 951
Size: 290 661
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 442 806 841 951
Size: 442 364 35 110
Fragments arranged by size:
442 364 110 35
BseYI C'CCAG_C
Cuts at: 0 31 951
Size: 31 920
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 951
Size: 154 797
BsiHKAI G_wGCw'C
Cuts at: 0 413 551 951
Size: 413 138 400
Fragments arranged by size:
413 400 138
Bsp1286I G_dGCh'C
Cuts at: 0 413 551 951
Size: 413 138 400
Fragments arranged by size:
413 400 138
BsrDI GCAATG_nn'
Cuts at: 0 770 951
Size: 770 181
BstXI CCAn_nnnn'nTGG
Cuts at: 0 111 216 249 951
Size: 111 105 33 702
Fragments arranged by size:
702 111 105 33
BstYI r'GATC_y
Cuts at: 0 902 951
Size: 902 49
Bsu36I CC'TnA_GG
Cuts at: 0 701 951
Size: 701 250
BtgI C'CryG_G
Cuts at: 0 356 951
Size: 356 595
BtsI GCAGTG_nn'
Cuts at: 0 318 951
Size: 318 633
DrdI GACnn_nn'nnGTC
Cuts at: 0 586 951
Size: 586 365
EaeI y'GGCC_r
Cuts at: 0 377 836 951
Size: 377 459 115
Fragments arranged by size:
459 377 115
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 484 951
Size: 484 467
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 803 951
Size: 85 718 148
Fragments arranged by size:
718 148 85
MscI TGG'CCA
Cuts at: 0 379 838 951
Size: 379 459 113
Fragments arranged by size:
459 379 113
MslI CAynn'nnrTG
Cuts at: 0 743 951
Size: 743 208
NcoI C'CATG_G
Cuts at: 0 356 951
Size: 356 595
NspI r_CATG'y
Cuts at: 0 201 575 731 951
Size: 201 374 156 220
Fragments arranged by size:
374 220 201 156
PasI CC'CwG_GG
Cuts at: 0 58 720 951
Size: 58 662 231
Fragments arranged by size:
662 231 58
PciI A'CATG_T
Cuts at: 0 571 951
Size: 571 380
PfoI T'CCnGG_A
Cuts at: 0 632 951
Size: 632 319
PsiI TTA'TAA
Cuts at: 0 515 951
Size: 515 436
SexAI A'CCwGG_T
Cuts at: 0 110 215 951
Size: 110 105 736
Fragments arranged by size:
736 110 105
SfcI C'TryA_G
Cuts at: 0 557 951
Size: 557 394
StyI C'CwwG_G
Cuts at: 0 356 928 951
Size: 356 572 23
Fragments arranged by size:
572 356 23
TatI w'GTAC_w
Cuts at: 0 182 951
Size: 182 769
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 111 144 951
Size: 111 33 807
Fragments arranged by size:
807 111 33
Enzymes that do cut:
AccI AflIII AleI BlpI BmgBI BpmI BsaAI BsaHI
BseRI BseYI BsgI BsiHKAI Bsp1286I BsrDI BstXI BstYI
Bsu36I BtgI BtsI DrdI EaeI EciI Eco57MI MscI
MslI NcoI NspI PasI PciI PfoI PsiI SexAI
SfcI StyI TatI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII Bme1580I BmrI BmtI BplI Bpu10I BpuEI BsaI
BsaBI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII
EagI EarI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MspA1I NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PflMI
PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TstI Tth111I XbaI XhoI XmaI XmnI ZraI