(Linear) (Six-base) MAPSORT of: KIBB7380.seq  Check: 2448  from: 1  to: 1008



KIBB7380.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:00  ..

AflII C'TTAA_G

Cuts at:      0     571    1008 
   Size:        571     437
 
ApoI r'AATT_y

Cuts at:      0     358     539    1008 
   Size:        358     181     469

  Fragments arranged by size: 

                 469     358     181
 
BamHI G'GATC_C

Cuts at:      0     979    1008 
   Size:        979      29
 
BanII G_rGCy'C

Cuts at:      0     241     527     974    1008 
   Size:        241     286     447      34

  Fragments arranged by size: 

                 447     286     241      34
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     267    1008 
   Size:        267     741
 
BbvCI CC'TCA_GC

Cuts at:      0     137     991    1008 
   Size:        137     854      17

  Fragments arranged by size: 

                 854     137      17
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     370     404    1008 
   Size:        370      34     604

  Fragments arranged by size: 

                 604     370      34
 
BclI T'GATC_A

Cuts at:      0     693     779    1008 
   Size:        693      86     229

  Fragments arranged by size: 

                 693     229      86
 
BglI GCCn_nnn'nGGC

Cuts at:      0     227     236    1008 
   Size:        227       9     772

  Fragments arranged by size: 

                 772     227       9
 
BglII A'GATC_T

Cuts at:      0     116    1008 
   Size:        116     892
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     630    1008 
   Size:        630     378
 
Bpu10I CC'TnA_GC

Cuts at:      0     137     991    1008 
   Size:        137     854      17

  Fragments arranged by size: 

                 854     137      17
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     958    1008 
   Size:        958      50
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     908    1008 
   Size:        908     100
 
BsaWI w'CCGG_w

Cuts at:      0      45    1008 
   Size:         45     963
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     194    1008 
   Size:        194     814
 
Bsp1286I G_dGCh'C

Cuts at:      0     241     527     974    1008 
   Size:        241     286     447      34

  Fragments arranged by size: 

                 447     286     241      34
 
BspHI T'CATG_A

Cuts at:      0     183    1008 
   Size:        183     825
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     241    1008 
   Size:        241     767
 
BstYI r'GATC_y

Cuts at:      0     116     979    1008 
   Size:        116     863      29

  Fragments arranged by size: 

                 863     116      29
 
Bsu36I CC'TnA_GG

Cuts at:      0     167    1008 
   Size:        167     841
 
BtsI GCAGTG_nn'

Cuts at:      0     949    1008 
   Size:        949      59
 
EarI CTCTTCn'nnn_

Cuts at:      0     401     623    1008 
   Size:        401     222     385

  Fragments arranged by size: 

                 401     385     222
 
EcoO109I rG'GnC_Cy

Cuts at:      0     352    1008 
   Size:        352     656
 
EcoRV GAT'ATC

Cuts at:      0     622    1008 
   Size:        622     386
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     328     493     571    1008 
   Size:        328     165      78     437

  Fragments arranged by size: 

                 437     328     165      78
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     101     133    1008 
   Size:        101      32     875

  Fragments arranged by size: 

                 875     101      32
 
MspA1I CmG'CkG

Cuts at:      0     673    1008 
   Size:        673     335
 
PasI CC'CwG_GG

Cuts at:      0     234     918    1008 
   Size:        234     684      90

  Fragments arranged by size: 

                 684     234      90
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     642    1008 
   Size:        642     366
 
PpuMI rG'GwC_Cy

Cuts at:      0     352    1008 
   Size:        352     656
 
PvuII CAG'CTG

Cuts at:      0     673    1008 
   Size:        673     335
 
SapI GCTCTTCn'nnn_

Cuts at:      0     401    1008 
   Size:        401     607
 
SmlI C'TyrA_G

Cuts at:      0     571     973    1008 
   Size:        571     402      35

  Fragments arranged by size: 

                 571     402      35
 
TatI w'GTAC_w

Cuts at:      0     471    1008 
   Size:        471     537
 
XmnI GAAnn'nnTTC

Cuts at:      0     308     311     551    1008 
   Size:        308       3     240     457

  Fragments arranged by size: 

                 457     308     240       3
 

 Enzymes that do cut:

    AflII     ApoI    BamHI    BanII     BbsI    BbvCI     BcgI     BclI
     BglI    BglII     BmrI   Bpu10I    BpuEI     BsaI    BsaWI    BseRI
 Bsp1286I    BspHI    BstXI    BstYI   Bsu36I     BtsI     EarI EcoO109I
    EcoRV     FalI    Hin4I   MspA1I     PasI    PflMI    PpuMI    PvuII
     SapI     SmlI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI     BbeI
    BciVI    BfrBI     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI
    BsaAI    BsaBI    BsaHI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I     BtgI
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PciI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI