(Linear) (Six-base) MAPSORT of: KIBB7379.seq  Check: 8420  from: 1  to: 540



KIBB7379.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:00  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     296     335     374     413     452     491     530     540 
   Size:        296      39      39      39      39      39      39      10

  Fragments arranged by size: 

                 296      39      39      39      39      39      39      10
            
 
ApoI r'AATT_y

Cuts at:      0     189     540 
   Size:        189     351
 
AvaI C'yCGr_G

Cuts at:      0     218     540 
   Size:        218     322
 
BanI G'GyrC_C

Cuts at:      0      87     540 
   Size:         87     453
 
BanII G_rGCy'C

Cuts at:      0      46     540 
   Size:         46     494
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     305     344     383     422     461     500     540 
   Size:        305      39      39      39      39      39      40

  Fragments arranged by size: 

                 305      40      39      39      39      39      39
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     223     540 
   Size:        223     317
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     262     265     540 
   Size:        262       3     275

  Fragments arranged by size: 

                 275     262       3
 
Bsp1286I G_dGCh'C

Cuts at:      0      46     540 
   Size:         46     494
 
BstYI r'GATC_y

Cuts at:      0     280     319     358     397     436     475     514     540 
   Size:        280      39      39      39      39      39      39      26

  Fragments arranged by size: 

                 280      39      39      39      39      39      39      26
            
 
EarI CTCTTCn'nnn_

Cuts at:      0      28      34     540 
   Size:         28       6     506

  Fragments arranged by size: 

                 506      28       6
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     223     296     335     374     413     452     491     530 
   Size:        223      73      39      39      39      39      39      39

Cuts at:    530     540 
   Size:         10

  Fragments arranged by size: 

                 223      73      39      39      39      39      39      39
                  10
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     292     324     331     363     370     402     409     441 
   Size:        292      32       7      32       7      32       7      32

Cuts at:    441     487     519     540 
   Size:         46      32      21

  Fragments arranged by size: 

                 292      46      32      32      32      32      32      21
                   7       7       7
 
MspA1I CmG'CkG

Cuts at:      0     274     540 
   Size:        274     266
 
PvuII CAG'CTG

Cuts at:      0     274     540 
   Size:        274     266
 
SapI GCTCTTCn'nnn_

Cuts at:      0      34     540 
   Size:         34     506
 
SfcI C'TryA_G

Cuts at:      0     168     540 
   Size:        168     372
 
StuI AGG'CCT

Cuts at:      0     263     540 
   Size:        263     277
 

 Enzymes that do cut:

     AcuI     ApoI     AvaI     BanI    BanII     BbsI     BpmI    BseRI
 Bsp1286I    BstYI     EarI  Eco57MI    Hin4I   MspA1I    PvuII     SapI
     SfcI     StuI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI